Porcine cells medium
A medium and basic medium technology, applied in the field of cell biology, can solve the problems of lack of medium, the influence of the number of cell passages, and the limited speed of cell proliferation in the cell growth state.
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Embodiment 1
[0074] Embodiment 1: the formula of porcine cell culture medium A, B, C
[0075] 1) The formula of porcine cell culture medium A:
[0076] Pig cell culture medium A consists of artificial medium 1 (the components are shown in Table 4), 5% (v / v) fetal pig serum and factor combination 1 (the components are shown in Table 5).
[0077] Table 4 artificial medium 1 formula:
[0078] name
Content (mg / L)
Calcium Chloride Anhydrous
200.00
L-serine
42.00
L-tryptophan
16.00
L-threonine
95.00
L-tyrosine sodium salt
104.00
Ferric nitrate
0.10
potassium chloride
400.00
L-valine
94.00
L-arginine hydrochloride
84.00
L-Glutamine
584.00
L-cystine hydrochloride
63.00
L-leucine
95.00
L-isoleucine
93.00
L-Histidine Hydrochloride
32.00
L-Lysine Hydrochloride
126.00
L-methionine
25....
Embodiment 2
[0087] Embodiment 2: the formula of porcine cell culture medium D, E, F, G
[0088] 1) The formula of pig cell culture medium D:
[0089] Pig cell culture medium D consists of artificial medium 2 (the components are shown in Table 6), 25% (v / v) fetal pig serum and factor combination 2 (the components are shown in Table 7).
[0090] Table 6 artificial medium 2 formula:
[0091] Compound name
Content (mg / L)
Calcium Chloride Anhydrous
240.00
L-serine
55.00
L-tryptophan
20.00
L-threonine
110.00
[0092] L-tyrosine sodium salt
123.00
Ferric nitrate
0.40
potassium chloride
600.00
L-valine
112.00
L-arginine hydrochloride
98.00
L-Glutamine
628.00
L-cystine hydrochloride
72.00
L-leucine
110.00
L-isoleucine
105.00
L-Histidine Hydrochloride
52.00
L-Lysine Hydrochloride
156....
Embodiment 3
[0101] Example 3: Preparation of primary porcine fibroblasts
[0102] Primary porcine fibroblasts can be prepared by traditional enzymatic digestion, the steps are as follows:
[0103] 1) Quickly scrub the pig fetuses that were killed (within 10 hours after being killed) three times with 70% alcohol, and put them into PBS+double antibody solution;
[0104] 2) Rinse the pig fetus with PBS in the ultra-clean workbench, and remove the head, limbs and internal organs of the pig fetus;
[0105] 3) Mince the pig fetal tissue treated in step 2) in a 60mm diameter plate, transfer the tissue fragments to a 50mL Erlenmeyer flask, and further chop into 1mm 3 Small fragments, take a small amount of tissue fragments and spread them on a 100mm Petri dish, add 10mL of culture medium (DMEM+15%FBS+double antibody), put in CO 2 Cultivate in an incubator (tissue block attachment method); add 3-5mL 0.25% trypsin digestion solution preheated at 39°C to the rest of the tissue fragments, put in CO...
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