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Human fibrinogen-like protein 2 prothrombinase immunogenicity peptide and use thereof
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A fibrin, immunogenic technology, applied in the direction of anti-enzyme immunoglobulin, peptide, antibody, etc.
Inactive Publication Date: 2013-09-18
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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[0005] So far, there are no reports on the glutamic acid-rich immunogenic peptide NPG-12 and its antibody against hfgl2 prothrombinase extracellular N-terminal 91 serine residue
Method used
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Embodiment 1
[0037] Synthesis of Example 1 Antigen
[0038] Using DNASTAR and HomoloGene software, according to the hydrophilicity, antigenicity, flexibility and exposure of hfgl2 prothrombinase extracellular N-terminal amino acid, using the Jameson-Wolf algorithm, through the computer antigen index prediction analysis, select the hydrophilicity, antigenicity NPG-12, a peptide with 12 consecutive amino acids with high index, good flexibility, rich in glutamic acid residues, exposed on the surface of the protein molecule, without homology within the species, and near the 91st serine residue The amino acid sequence is: Glu-Glu-Val-Phe-Lys-Glu-Val-Gln-Asn-Leu-Lys-Glu. According to its amino acid sequence, PSSM-8 automatic peptide synthesizer was used to synthesize the peptide by FOMC method. NPG-12-BSA complete antigen was synthesized by glutaraldehyde coupling method with BSA as carrier protein. After the antigen synthesis was completed, it was stored in a -70°C refrigerator for later use....
Embodiment 2A
[0039] Embodiment 2 Preparation of Anti-NPG-12 antibody
[0040] (1) Animal immunity
[0041] Anti-NPG-12 antibody was prepared by immunizing male New Zealand white rabbits (clean grade, weighing 2.5-3 kg) with the prepared antigen together with complete or incomplete Freund's adjuvant. Set up the immunization group and the pseudo-immunization group. The immunization group: take 500 μg of antigen per rabbit for the initial immunization, and 250 μg of antigen per rabbit for subsequent immunization, and add an equal volume of Freund’s adjuvant to both. Incomplete Freund's adjuvant, fully emulsified, injected subcutaneously on the back and hind legs of rabbits. Booster immunization every two weeks, a total of 5 times. Sham immunization group: Sham immunization with normal saline plus Freund's adjuvant, the dose and method were the same as the immunization group.
[0042] (2) Collection of serum samples
[0043] From the first immunization, 1 ml of blood was drawn from the vei...
Embodiment 3
[0046] Example 3 Establishment of cell model expressing hfgl2 prothrombinase
[0047] Human acute monocytic leukemia cell line THP-1 cells (purchased from American Standard Biological Collection) were stimulated with 20ng / ml IFN-γ for 12 hours, and the cells could express hfgl2 prothrombinase.
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Abstract
The invention provides a human fibrinogen-like protein 2 prothrombinase immunogenicity peptide and a use thereof. The human fibrinogen-like protein 2 prothrombinase immunogenicity peptide comprises 12 continuous glutamic acid-rich amino acids near by a serine residue located at an exocellular amino terminal locus 91 of human fibrinogen-like protein 2 prothrombinase. An amino acid sequence of the human fibrinogen-like protein 2 prothrombinase immunogenicity peptide is Glu-Glu-Val-Phe-Lys-Glu-Val-Gln-Asn-Leu-Lys-Glu. A monoclonal antibody of the human fibrinogen-like protein 2 prothrombinase immunogenicity peptide can be prepared by an immunological method and can be used as a novel and specific inhibitor of human fibrinogen-like protein 2 prothrombinase. The monoclonal antibody can be used for research of adjustment on blood coagulation promotion functions of human fibrinogen-like protein 2 prothrombinase. The monoclonal antibody prepared according to the human fibrinogen-like protein 2 prothrombinase immunogenicity peptide is beneficial for clinic intervention of disease associated with microcirculatory disturbance.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a human-derived fibrinogen-like protein 2 prothrombinase immunogenic peptide NPG-12 and an antibody prepared by using the peptide, and the antibody as human-derived fibrinogen Use of specific inhibitors of protein 2 prothrombinase. Background technique [0002] The activation of coagulation pathway mediated by human fibrinogen-like protein 2 prothrombinase (Human Fibrinogen-like Protein 2 Prothrombinase, fgl2 prothrombinase) plays an important role in many microcirculation disorders. Different from the traditional intrinsic and extrinsic coagulation pathways, it does not rely on factor Xa and directly catalyzes the conversion of prothrombin into active thrombin from "bypass", thereby rapidly initiating the coagulation reaction and leading to in situ thrombus formation in microvessels. A large number of studies have confirmed that in bacterial and viral infectious disease...
Claims
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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C07K16/40A61K39/395A61P7/02
Inventor 王朝晖刘坤李文珠苏冠华陈宇王敏王珏
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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