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Fluorescent chemical sensor for detecting [H plus] and preparation method and usage thereof

A chemical sensor and fluorescence technology, which is applied in chemical instruments and methods, chemiluminescence/bioluminescence, and analysis by making materials undergo chemical reactions, to achieve the effects of simple configuration, fast fluorescence response speed, and large amplitude

Active Publication Date: 2013-09-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Sensitive carriers commonly used in pH fluorescent sensors are mainly fluoresceins [1-3] (aminofluorescein [1] Anal. Chem. , 1982, 54(20): 821-823, fluorescein isothiocyanate [2] Anal. Chem. Acta. , 1998, 367: 159-165, Acrylamidofluorescein [3] Anal. ., 1986, 58(3): 1427-1430), rhodamines [4] (Tetramethylrhodamine used by McNamara et al. Anal. Chem. , 2001, 73(4): 3240-3246), and some metal complexes [5-6] (such as ruthenium complexes [5] Anal. Chem. , 1998, 70(2): 3892-3897 and europium complexes [6] Sens. Actuators B , 2001, 74(2): 200-206 are also used as fluorescent carriers for pH sensors, but changes in pH may cause complex decomposition

Method used

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  • Fluorescent chemical sensor for detecting [H plus] and preparation method and usage thereof
  • Fluorescent chemical sensor for detecting [H plus] and preparation method and usage thereof
  • Fluorescent chemical sensor for detecting [H plus] and preparation method and usage thereof

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preparation example Construction

[0025] Detection [H + ] The preparation and use method of the fluorescent chemical sensor is: weigh 10,11,12,13-tetraphenylphenanthro[9',10':4,5]imidazol[1,2-a]pyridine or 9, 11,12,14-Tetraphenyldibenzo[f,h]imidazo[1,2-b]isoquinoline, prepared in organic solvent to 1×10 -5 mol / l standard detection solution, i.e. fluorescent chemical sensor, when the solution to be tested is added to the standard detection solution, the [H + ]value.

[0026] The organic solvent is: one or more of cyclohexane, tetrahydrofuran, 1,4-dioxane, methylene chloride, acetonitrile, toluene, dimethylformamide or dimethylacetamide.

[0027] The solution to be tested is the acidic food solution of acid sewage, egg yolk, cheese, tuna, flounder, cheese or soybean milk in chemical plants.

[0028] The present invention will be further described below in conjunction with embodiment

[0029] (1) Configuration of standard detection solution for 10,11,12,13-tetraphenylphenanthrene[9',10':4,5]imidazo[1,2-a]pyr...

Embodiment 1

[0036] Use a pipette to draw a concentration of 1×10 -5 2.5 ml standard detection solution of 10,11,12,13-tetraphenylphenanthrene[9',10':4,5]imidazo[1,2-a]pyridine in mol / l was placed in a cuvette, Determination of 10,11,12,13-tetraphenylphenanthrene[9',10':4,5]imidazo[1,2-a]pyridine in the absence of external [H + ] in the presence of UV and fluorescence spectra, record the output data.

Embodiment 2

[0038] Pipette the concentration of 1 × 10 -5 mol / l 10,11,12,13-tetraphenylphenanthrene[9',10':4,5]imidazo[1,2-a]pyridine standard detection solution 2.5 ml in two cuvettes , and then use a pipette gun to draw 51 microliters of [H + ]=1×10 -1Add mol / l acidic sewage or egg yolk solution into two cuvettes respectively, vibrate to mix them evenly, at this time the [H of the mixed solution + ]=2.0×10 -3 mol / l, respectively measure the ultraviolet spectrum and fluorescence of 10,11,12,13-tetraphenylphenanthro[9',10':4,5]imidazo[1,2-a]pyridine at this hydrogen ion concentration Spectra, record data.

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Abstract

The invention discloses a fluorescent chemical sensor for detecting [H plus] and a preparation method and usage thereof. The fluorescent pH sensor adopts 10,11,12,13-tetraphenylphenanthro[9',10':4,5]imidazole[1,2-a]pyridine or 9, 11,12,14-tetraphenyldibenzo[f,h]imidazole[1,2-b]isoquinoline as a fluorescent material, which is prepared into 1 multiplied by 10 to 5mol / l of standard testing solution, and when acidic solution is added into the standard testing solution, an ultraviolet spectrum can detect the increase of the intensity of an ultraviolet absorption curve and a fluorescence spectrum can detect the decrease of the intensity of a fluorescence emission curve and the response of emission peak blue shift. The invention is characterized in that: the standard testing solution is easy to prepare, consumption is little, the speed of fluorescence response is high, the amplitude is high, and common metal ions cannot interfere with the fluorescent chemical sensor. The fluorescent chemical sensor can be used for testing the acidic sewage of chemical plants and acid food such as yolks, cream, tunas, flatfishes, cheese or soybean milk.

Description

technical field [0001] The present invention relates to a detection [H + ] Fluorescent chemical sensors and methods for their preparation and use. Background technique [0002] Acid-base measurement is closely related to modern industry, agriculture, medicine, bioengineering, environment and scientific research, so pH sensors have attracted much attention. Traditional pH chemical sensors are mainly various glass electrodes. With the development of modern science and technology, especially the rapid development of modern life science, environmental science and high-tech fields, some difficulties often arise when using traditional glass electrodes for pH measurement. [0003] Changes in the fluorescence or light absorption properties of some organic compounds with pH can be used to indicate changes in acidity and alkalinity in the target medium. This pH measurement method based on optical signal changes can make up for the above-mentioned shortcomings of glass electrodes, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06G01N21/76
Inventor 李继超李熙晖吕萍王彦广
Owner ZHEJIANG UNIV
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