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Strain for producing ornithine and method for biologically synthesizing ornithine with same

A technology of ornithine and bacterial strains, which is applied in the fields of biosynthesis of ornithine, food, biological preparation of ornithine, cosmetics, and medicine to achieve the effect of high-efficiency production

Inactive Publication Date: 2012-05-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The synthesis method is the preparation method of early research on ornithine. The process is complicated and the cost is high. Both D-ornithine and L-ornithine are available, which are not easy to separate, so they have been eliminated.
The enzymatic method is to generate L-ornithine from arginine under the action of arginase. The product of this method is high in purity, but the price is expensive, and arginine and arginase are not easy to obtain, so it is limited to laboratory preparation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The fermentation culture of the bacterial strain No. SK23.002 of embodiment 1

[0027] Use strain SK23.002, in a medium containing 0.5% of L-Arg, 1% of glucose, 0.5% of yeast extract, 0.5% of peptone, 0.1% of inorganic nitrogen source, and with or without a small amount of inorganic salt, etc., to adjust the pH to 6.0 , 37°C, cultured at 190 rpm for 22 hours, refrigerated and centrifuged to obtain resting cells with arginase activity.

Embodiment 2

[0028] Example 2 Transformation reaction

[0029] Using the cells obtained in Example 1, in 100mM acetate buffer solution (HAc-NaAc, pH 6.0) containing arginine mass concentration of 50g / L and resting cell mass of 0.1-10g / L, at 37°C The conversion reaction takes 5-40h to synthesize ornithine.

Embodiment 3

[0030] Embodiment 3 Determination of ornithine output

[0031] The reaction solution carried out in Example 2 was inactivated (TCA precipitation), diluted, and quantified by Agilent liquid chromatography for arginine and ornithine, respectively. The analysis conditions are as follows: instrument model: Agilent 1100, chromatographic column: (250*4.6) mm 5 μm ODS HYPERSIL, column temperature: 40°C.

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Abstract

The invention provides a strain for producing ornithine and a method for biologically synthesizing ornithine with the same, belonging to the food biotechnology field. The strain and the method have the following beneficial effects: the invention relates to rummeliibacillus pycnus (SK23-002) screened from the soil; the collection number of rummeliibacillus pycnus is CCTCC NO:M2011466; the sporeformer is taken as the fermentation strain, glucose is taken as the carbon source and yeast cream and peptone are taken as nitrogen sources to form a fermentation medium to ferment ornithine or a thalluscontaining arginase, and the thallus is added to 5-20% of arginine solution to synthesize ornithine through transformation; through detection, the transformation rate can be more than 80%; the fermentation process can be independent of arginine induction; ornithine produced by the method is safe and reliable, is a functional product with great market potential and is widely applied to such industries as food, cosmetics, medicines and the like; and the method can be used for efficiently producing ornithine and is suitable for large scale production.

Description

technical field [0001] The invention relates to a new microbial strain capable of producing ornithine and a method for cultivating and fermenting it to produce ornithine or bacteria containing arginase, and using the bacteria to biologically prepare ornithine, which belongs to food biology In the field of technology, it is widely used in food, cosmetics, medicine and other industries. More specifically the present invention relates to soil-derived Rummeliibacillus pycnus (SK23.002), the deposit number is CCTCC NO: M 2011466, and the strain is used to biosynthesize ornithine (ornithine). Background technique [0002] In recent years, it has been found that ornithine can stimulate the pituitary gland to secrete growth hormone, promote protein synthesis and metabolism of sugar and fat (basic metabolism), and ingesting ornithine in conjunction with exercise can reduce body fat accumulation, enhance muscle and physical strength, and play a role in weight loss In addition, orn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P13/10C12R1/01
Inventor 张涛江波王莹缪铭沐万孟
Owner JIANGNAN UNIV
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