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Method for studying metabolic difference of transgenic rice and non-transgenic rice

A transgenic rice, non-transgenic technology, applied in the field of analytical chemistry, can solve the problems of experimental data deviation from the true value, affect the credibility of the final result, and mass spectrometry response signal deviation, etc., to save identification time, fast scanning speed, and avoid human error Effect

Inactive Publication Date: 2014-08-13
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
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Problems solved by technology

Since the pretreatment process is carried out in multiple steps, it is easy to introduce errors in the sample preparation process; if the number of samples is relatively large, the mass spectrometry will run for a long time, and due to the attenuation effect of the electrospray ion source itself, the mass spectrometry response signal of the sample will have a certain deviation
This will cause the experimental data to deviate from the true value and affect the credibility of the final result

Method used

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  • Method for studying metabolic difference of transgenic rice and non-transgenic rice
  • Method for studying metabolic difference of transgenic rice and non-transgenic rice
  • Method for studying metabolic difference of transgenic rice and non-transgenic rice

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Experimental program
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Embodiment 1

[0023] 1. Rice sample collection

[0024] The transgenic rice sample is a Marker-free bivalent insect-resistant strain material; including 3 strains, namely N6080, N6049, and N6130, which are excellent strains transformed by Minghui 86 as a recipient, after continuous multi-generation selfing and field selection Tie. Both the genetically modified rice and the non-transgenic rice Minghui 86 are planted in Hainan, sowed and harvested at the same time. Rice samples were collected and stored in a -80°C refrigerator.

[0025] 2. Analysis method

[0026] 2.1 Pretreatment of rice seed samples

[0027] The rice seed samples were taken out from the -80 °C refrigerator and waited for their temperature to return to room temperature in a desiccator. Each rice seed is dehulled, ground in a mortar, and passed through a 60-mesh sieve to obtain a powder with uniform particle size. Divide the rice powder into plastic tubes and set aside.

[0028] Weigh 5 parts of N6080 or N6049 (0.1500 g...

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Abstract

The invention discloses a method for rice metabolic profile analysis and metabolic difference research, which uses liquid chromatography-mass spectrometry to analyze rice seed extracts to obtain rice metabolic profile, and then uses multivariate statistical methods to study transgenic rice and non-transgenic rice. The overall difference in the metabolic profile data of transgenic rice, and the discovery of compounds that contribute significantly to the differences in metabolic phenotypes, provide a basis for the unexpected effects and safety evaluation of transgenic rice. The analysis method of the invention is simple, fast and has good repeatability, and is suitable for batch analysis of actual samples.

Description

technical field [0001] The invention relates to the field of analytical chemistry, and is a method for studying metabolic differences between transgenic and non-transgenic rice based on rice metabolic profile of metabolomics technology. Background technique [0002] The rapid development of transgenic technology has sparked debates on the impact of transgenic plants on human health and the ecological environment. At present, people's concerns about the safety of genetically modified plants are mainly reflected in the following aspects: (1) How toxic the foreign gene is; (2) Whether it is allergic; (3) Whether it produces toxic substances; (4) Whether it affects the nutritional value. Influence. In order to ensure the safety of genetically modified plants, many countries including the United States, the European Union, Japan and my country, as well as some international organizations such as FAO and WHO have formulated a series of management measures for the safety of geneti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 许国旺常玉玮赵春霞路鑫周佳赵燕妮
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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