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Fullerenol solid lipid nano-particles, preparation method thereof, and application thereof

A technology of solid lipid nanometer and fullerol, applied in the field of radiation protection, can solve the problem of no fullerene or its derivatives, etc., and achieve the effect of strong free radical scavenging ability, high stability and low toxicity effect.

Inactive Publication Date: 2012-06-13
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, there is no report on the application of fullerene or its derivatives as radiation protection drugs, nor the use of polyhydric fullerene C60(OH) X Carrying to the nucleus as a free radical scavenger report

Method used

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  • Fullerenol solid lipid nano-particles, preparation method thereof, and application thereof
  • Fullerenol solid lipid nano-particles, preparation method thereof, and application thereof
  • Fullerenol solid lipid nano-particles, preparation method thereof, and application thereof

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Embodiment 1

[0044] Cell culture: culture cells in RPMI-1640 medium (containing 10% inactivated fetal bovine serum and double antibody, working concentration: penicillin 100U / ml, streptomycin 0.1mg / ml), incubator CO 2 The concentration is 5%, the temperature is 37°C, the cells are subcultured once every 2-3 days, and they are used for experiments after three generations of resuscitation.

[0045] Preparation and characterization of water-soluble fullerenol: Weigh 100mg of C60, dissolve it in 50mL of benzene and stir overnight, add 2mL of 2mol / L NaOH solution, add 5 drops of 40% TBAH solution under magnetic stirring, add dropwise 0.5mL of 30% H 2 o 2 Solution, when the benzene liquid turns colorless and the water phase turns brown, the reaction is complete. Separate the organic phase and the aqueous phase with a separatory funnel, wash the separatory funnel twice with 2-3ml of water, and combine the aqueous phase, that is, the fullerenol solution. Add 20-30ml of methanol to make it preci...

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Abstract

The invention belongs to the field of radiation protection, and specifically relates to nucleus-targeting fullerenol solid lipid nano-particles with a radiation protection function. The fullerenol solid lipid nano-particles comprises: stearic acid, cholesterol, lecithin, corn oil, Tween-80, fullerenol powder, and diethylstilbestrol. According to the invention, stearic acid, cholesterol, lecithin, corn oil, fullerenol powder, and diethylstilbestrol are sufficiently dissolved in ethanol; the mixture is well mixed; ethanol is removed by rotary evaporation; the mixture is slowly dropped into an aqueous solution of Tween-80 with a same temperature; when dropping is finished, the mixture is stirred and well mixed, such that C60(OH)24-SLN-E primary emulsion is obtained; the C60(OH)24-SLN-E primary emulsion is subject to high-pressure homogenizing, and is cooled, such that a required product is obtained. The fullerenol solid lipid nano-particles provided by the invention can penetrate various biological barriers and nuclear membranes, and carry fullerenol into nuclei with high efficiency. Therefore, free radicals in the nuclei can be removed, such that the radiation protection function can be realized.

Description

technical field [0001] The invention belongs to the field of radiation protection, in particular to a nucleus-targeted fullerenol solid lipid nanoparticle with radiation protection function. Background technique [0002] Nuclear energy is widely used in the fields of national defense, industry, agriculture and medicine. While benefiting mankind, it also brings serious nuclear radiation crisis. In March 2011, a magnitude 9.0 earthquake in Japan caused a high-temperature nuclear fuel leak at the Fukushima nuclear power plant. The damage to the body caused by ionizing radiation and its protection have received great attention from governments and researchers. The radiation protection of radioisotopes in industrial, agricultural and other applications is very important, and the protection of normal tissues and organs in tumor radiation therapy is also very important. Ionizing radiation is the general term for all radiation that can cause material ionization. It acts on the body...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K33/00A61P39/00A61P39/06
Inventor 许玉杰刘敏杨百霞孙俊杰
Owner SUZHOU UNIV
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