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Active extract containing isocarthamidin 7-O-beta-D-glucuronidase and use thereof

A technology of glucuronide and isosaffron, applied in the application of cerebrovascular diseases, in the field of heart treatment, can solve the problem of the absence of isosaffin 7-O-β-D-glucuronide, the absence of And other issues

Inactive Publication Date: 2012-06-13
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are research reports that the plant extracts of safflower, Scutellaria barbata, and Scutellaria baicalensis containing isoflavin have cardiovascular and cerebrovascular activities, but as a monomer to study the activity of cardiovascular and cerebrovascular aspects, there is no evidence of any effect. related reports
[0006] At the same time, so far, there is still no method for extracting and preparing isoflavin 7-O-β-D-glucuronide with simple process, low cost and suitable for industrialization and the use of the extract in treating Related reports on clinical application in cardiovascular disease and cerebrovascular disease

Method used

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  • Active extract containing isocarthamidin 7-O-beta-D-glucuronidase and use thereof
  • Active extract containing isocarthamidin 7-O-beta-D-glucuronidase and use thereof
  • Active extract containing isocarthamidin 7-O-beta-D-glucuronidase and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 《Example 1》Macroporous resin prepares isosaffin 7-O-β-D-glucuronide

[0019] Add the stems and leaves of Scutellaria baicalensis into 70% ethanol aqueous solution with 8 times the amount, soak for 2 hours, heat to reflux state, and reflux for 1 hour, filter; add 6 times the amount of 70% ethanol aqueous solution to the filter residue, and heat to reflux State and reflux for 1 hour, filter; repeat this process 1 time. Combine the filtrates, concentrate under reduced pressure to recover ethanol, and obtain isosaffin 7-O-β-D-glucuronide extract.

[0020] Put 30g of isosaffin 7-O-β-D-glucuronide dry extract in 100ml of distilled water, heat to 80°C and keep stirring to make isosaffin 7-O-β-D-glucuronide The acid glycosides are completely dissolved, and the yellow-brown clear solution is obtained by suction filtration while it is hot. Let it stand still and cool to 20-40°C. Add the solution to the head end of the medium-polarity macroporous adsorption resin (DM-301), and el...

Embodiment 2

[0021] "Example 2" Polyamide Refined Isofoscin 7-O-β-D-Glucuronide

[0022] Add scutellaria baicalensis stems and leaves to 10 times the amount of water, soak for 1 hour, heat to reflux state, and reflux for 1 hour, filter; add 5 times the amount of water to the filter residue, heat to reflux state, reflux for 1 hour, and filter; repeat this process 1 Second-rate. The filtrates were combined and concentrated under reduced pressure to obtain the extract of isosaffin 7-O-β-D-glucuronide.

[0023] Put 30g of isosaffin 7-O-β-D-glucuronide dry extract in 100ml of distilled water, heat to 80°C and keep stirring to make isosaffin 7-O-β-D-glucuronide Glycosides were completely dissolved, filtered while hot to obtain a brown clear solution, let stand, cooled to 20-40°C, added the solution to the head of the polyamide column, eluted with distilled water to remove part of the water-soluble impurities and pigments, and used 20 -40% ethanol aqueous solution for elution, thin-layer detect...

Embodiment 3

[0024] "Example 3" Preparation of Isosafflorin 7-O-β-D-glucuronide active extract tablet

[0025] Described tablet is prepared according to following formula (all contents used are weight percent):

[0026]

[0027] Mix the isosafflorin 7-O-β-D-glucuronide extract, microcrystalline cellulose, and part of carboxymethyl starch sodium, add PVP-k30 ethanol solution to make a soft material, and pass through a 20-mesh sieve Granules, air-dried at 60°C, granulated, added sodium carboxymethyl starch and magnesium stearate, mixed evenly, and compressed into tablets.

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Abstract

The invention relates to a preparation method and use of an active extract containing an isocarthamidin 7-O-beta-D-glucuronidase. The active extract is extracted from scutellaria plants and further separated and purified by polyamide or macroporous resin; and experimental researches indicate the active extract containing the isocarthamidin 7-O-beta-D-glucuronidase and a medical composition thereof have the functions of preventing and treating cardiovascular and cerebrovascular diseases and are expected to be developed into effective medicines for use in clinic.

Description

Technical field: [0001] The invention relates to an active extract obtained from plants such as Scutellaria baicalensis, a preparation method thereof and an application in treating heart and cerebrovascular diseases. Background technique: [0002] Cardiovascular and cerebrovascular diseases are collectively referred to as cardiovascular diseases and cerebrovascular diseases, and generally refer to ischemia or hemorrhage in the heart, brain and systemic tissues caused by hyperlipidemia, blood viscosity, atherosclerosis, high blood pressure, etc. Generic name for disease. Cardiovascular and cerebrovascular diseases seriously endanger human health, especially the common diseases of middle-aged and elderly people over 50 years old. The number of people who die from cardiovascular and cerebrovascular diseases in the world is as high as 15 million every year, ranking first among various causes of death. Atherosclerosis (AS) is one of the main causes of inducing and promoting the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08A61K31/7048A61P9/10A61P9/14
Inventor 何春年肖培根彭勇许利嘉刘海波肖伟
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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