The invention relates to a sucrose-free drink with effects of reducing lipid, losing weight, eliminating meat, clearing heat, invigorating the spleen, removing dampness and lowering blood glucose as well as preparation method of the sucrose-free drink, belongs to the field of beverage production, and provides the sucrose-free drink with effects of reducing lipid, losing weight, eliminating meat, clearing heat, invigorating spleen, removing dampness and lowering blood glucose. The sucrose-free drink with effects of reducing lipid, losing weight, eliminating meat, clearing heat, invigorating spleen, removing dampness and lowering blood glucose is prepared from the following components in parts by mass: 8-30 parts of hawthorn, 1-20 parts of fructus momordicae, 1-15 parts of mulberry leaves, 1-8 parts of lotus leaves, 1-15 parts of rice beans, 1-5 parts of herba portulacae, 1-7 parts of radix glycyrrhizae, 1-15 parts of fried white hyacinth beans, 1-5 parts of honeysuckle flpwers, 1-7 parts of selfheal, 1-10 parts of poria cocos, 1-3 parts of boat-fruited sterculia seeds, 1-9 parts of herba lophatheri, 1-5 parts of chrysanthemum flowers, 1-3 parts of dandelion, 1-3 parts of vermilion,1-6 parts of leaves of paniculate microcos, 1-3 parts of semen cassia and a proper amount of water. The drink has the advantages of reducing lipid, losing weight, eliminating meat, clearing heat and toxic materials, invigorating the spleen, removing dampness, promoting digestion to eliminate stagnation, cooling blood, promoting salivation, moistening intestines, moistening lung, reducing blood glucose, decreasing internal heat and the like; and raw materials are convenient to obtain and low in cost, and production is convenient.