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Kit for separating human umbilical cord blood stem cells and its using method

A technology of human umbilical cord blood and stem cells, which is applied in the field of human umbilical cord blood stem cell treatment solution, can solve the problems of restricting the application of umbilical cord blood stem cells, low purity of umbilical cord blood stem cells, and complicated separation steps, so as to solve the bottleneck of stem cells, less impurities, and production low cost effect

Inactive Publication Date: 2012-06-13
葛龙海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0021] Although human umbilical cord blood stem cells have a wide range of clinical application prospects, the existing separation methods have various shortcomings, such as complicated separation steps, long time-consuming, high separation cost, low purity and poor activity of the prepared umbilical cord blood stem cells, etc., which limit application of umbilical cord blood stem cells

Method used

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  • Kit for separating human umbilical cord blood stem cells and its using method
  • Kit for separating human umbilical cord blood stem cells and its using method

Examples

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Embodiment 1

[0050] A kit for isolating human umbilical cord blood stem cells, the kit consists of liquid A, liquid B and liquid C,

[0051] Solution A: 0.2mmol / L puerarin, 0.3μmol / L phenylethyl 4-hydroxycinnamate, 5mmol / L PBS, pH7.4

[0052] B liquid: mass / volume percentage concentration is 8% hydroxyethyl starch solution

[0053] Liquid C: a dextran-diatrizoate solution with a density of 1.077 g / mL.

Embodiment 2

[0055] A kit for isolating human umbilical cord blood stem cells, the kit consists of liquid A, liquid B and liquid C,

[0056] Solution A: 0.5mmol / L puerarin, 1.0μmol / L 4-hydroxyphenylethyl cinnamate, 20mmol / L PBS, pH7.4

[0057] Liquid B: a methylcellulose solution with a mass / volume concentration of 0.5%

[0058] Liquid C: Percoll with a density of 1.076 g / mL.

Embodiment 3

[0060] A kit for isolating human umbilical cord blood stem cells, the kit consists of liquid A, liquid B and liquid C,

[0061] Solution A: 0.3mmol / L puerarin, 0.5μmol / L phenethyl 4-hydroxycinnamate, 10mmol / L PBS, pH7.4

[0062] Liquid B: 10% hydroxyethyl starch solution in mass / volume percentage concentration

[0063] Liquid C: a dextran-diatrizoate solution with a density of 1.077 g / mL.

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Abstract

The invention relates to a kit for separating human umbilical cord blood stem cells and its using method. The kit is composed of a solution A, a solution B and a solution C, wherein, the solution A is added with appropriate amounts of puerarin and 4-hydroxycinnamic acid phenethyl ester. The invention also relates to a human umbilical cord blood stem cell treatment liquid, which contains the human umbilical cord blood stem cells prepared by the kit of the invention. The kit provided in the invention can rapidly and efficiently separate stem cells from umbilical cord blood, and is in favor of popularizing stem cell treatment technologies.

Description

technical field [0001] The invention relates to a kit for isolating human umbilical cord blood stem cells and a using method thereof. The present invention also relates to a treatment liquid for human umbilical cord blood stem cells, which comprises human umbilical cord blood stem cells prepared by using the kit of the present invention. Background technique [0002] Stem cells are primitive cells with the potential of self-replication and multi-directional differentiation. They are the origin cells of the body and the primitive cells that form various tissues and organs of the human body. Under certain conditions, it can differentiate into a variety of functional cells or tissues and organs, which are called "universal cells" in the medical field. [0003] Stem cells are especially important for early human development, and multipotent stem cells are also found in children and adults. Take for example one of the stem cells we are most familiar with, hematopoietic stem cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0789A61K35/44A61K35/14A61P27/02A61P25/02A61P9/10A61P25/00A61P25/28A61P21/00A61P3/10A61P19/08A61K35/51
Inventor 葛龙海
Owner 葛龙海
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