Detection reagent, reagent strip and kit for semiquantitative detection of clenobuterol hydrochloride through colloidal gold fusion latex method, and preparation methods thereof

A technology for semi-quantitative detection of clenbuterol hydrochloride, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of uneven color development, low reagent cost, slow color development, etc. Accurate effect

Active Publication Date: 2012-06-27
爱若维生物科技(苏州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above problems, the present invention has designed a colloidal gold fusion latex method semi-quantitative detection of clenbuterol hydrochloride detection reagents, reagent strips and test kits and preparation methods thereof, the detection reagents overcome the colloidal gold competitive inhibition of immunochromatography The method detects the defects of slow color development and uneven color development of critical samples. At the same time, the reagent prepared by this method is low in cost, can be used for multi-level semi-quantitative detection of clenbuterol hydrochloride content, and is easy to use and easy to operate.

Method used

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  • Detection reagent, reagent strip and kit for semiquantitative detection of clenobuterol hydrochloride through colloidal gold fusion latex method, and preparation methods thereof
  • Detection reagent, reagent strip and kit for semiquantitative detection of clenobuterol hydrochloride through colloidal gold fusion latex method, and preparation methods thereof
  • Detection reagent, reagent strip and kit for semiquantitative detection of clenobuterol hydrochloride through colloidal gold fusion latex method, and preparation methods thereof

Examples

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Effect test

Embodiment 1

[0065] A kind of detection reagent of the semi-quantitative detection clenbuterol hydrochloride of colloidal finance and latex method, the preparation method of described reagent comprises the following steps:

[0066] (1) Preparation of colloidal gold

[0067] Take 100mL of chloroauric acid aqueous solution with a mass fraction of 0.01%, heat it to boiling, add 2mL of trisodium citrate with a mass fraction of 1% under stirring, continue to boil for 15 min, and dilute to 100mL with distilled water after cooling;

[0068] (2) Preparation and purification of colloidal gold markers

[0069] Take 100 mL of the colloidal gold prepared in the above step (1) and adjust the pH to 8.0 with 0.1 mol / L potassium carbonate solution; add 1.5 mg of anti-clenbuterol monoclonal antibody while stirring, stir for 20 min, then add 25 mg dropwise mg / ml polyethylene glycol 20000 (PEG20000) 2ml, stirred for 15min; centrifuged at 20000r / min for 15min, discarded the supernatant, added 10ml of pH7.4 P...

Embodiment 2

[0079] A clenbuterol hydrochloride detection reagent strip, the reagent strip comprises the nitrocellulose membrane detection reagent prepared in the above embodiment 1, and the preparation method of the reagent strip is as follows:

[0080] (1) Paste the gel nitrocellulose membrane detection reagent prepared by the method in Example 1 above in the middle of the supporting plastic plate;

[0081] (2) Paste an absorbent pad (whatman company) above the position where the nitrocellulose membrane is fixed on the supporting plastic plate, and the absorbent pad covers the edge of the nitrocellulose membrane by 0.5-1.5 mm;

[0082] (3) Paste the glass fiber sample pad marked with colloidal gold-antibody conjugate and the polyester film marked with latex-antibody conjugate prepared in step (4) of Example 1 above under the nitrocellulose membrane; Paste the glass fiber sample pad below the polyester film to cover the polyester film by 0.5-1.5 mm;

[0083] (4) After flattening the prod...

Embodiment 3

[0086] Such as Figure 1-5 As shown, a clenbuterol hydrochloride detection kit includes a test card 1 and a test strip; the test strip is divided into a handle area 9, an observation area 10, a marking area and a sample adding area 15 from top to bottom;

[0087]The bottom plate 8 of the observation area 10 is provided with a nitrocellulose membrane, and one end of the nitrocellulose membrane near the handle area is provided with a control line 104 coated with a goat anti-mouse antibody, and the other end is provided with a first CL-BSA conjugate in turn. A detection line 101 , a second detection line 102 , and a third detection line 103 .

[0088] The test card 1 includes a card cover 11 and a box-shaped card body 12. One end of the card cover 11 is provided with a funnel-shaped sample injection hole 2 for dripping test samples, and the middle part of the card cover 11 is provided with a hole for observing the test results. The strip-shaped observation hole 3, the side corre...

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Abstract

The invention discloses a detection reagent, a reagent strip and a kit for the semiquantitative detection of clenobuterol hydrochloride through a colloidal gold fusion latex method, and preparation methods thereof. Colloidal gold preparation, colloidal gold marker preparation and purification, latex particle marking, colloidal gold and latex maker immobilization, cellulose nitrate membrane coating, reagent strip preparation, and detection kit preparation are comprised in the invention. According to the invention, the cost is low, the accuracy is high, the stability is strong, the color development is rapid, uniform and stable, the use is convenient, the operation is simple, and the organic unification of a complex principle and the simple operation can be really achieved; and the kit has the characteristics of convenient preservation and long validity period.

Description

technical field [0001] The invention relates to a detection reagent of clenbuterol hydrochloride, specifically a detection reagent, a reagent strip, a test kit and a preparation method thereof for the semi-quantitative detection of clenbuterol hydrochloride by the colloidal financial latex method, belonging to the clenbuterol hydrochloride Rentero detection reagent field. Background technique [0002] Clenbuterol hydrochloride (Clenbuterol, CL), also known as clenbuterol, the molecular formula is C 12 h 18 C 12 N 2O ·HCl, the drug names are crotonin, ammonia, etc., are white or off-white crystalline powder, odorless, bitter, soluble in water, methanol, ethanol, slightly soluble in acetone, chloroform, insoluble in ether and Benzene, chemically stable; it is a synthetic β-adrenergic receptor agonist, which has the effect of dilating the bronchi, and is often used to prevent and treat lung diseases such as asthma and emphysema. When its application dose reaches 5-10 times...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 杨致亭王增祺付伟光王好玉单永德
Owner 爱若维生物科技(苏州)有限公司
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