Detection reagent, reagent strip and kit for semiquantitative detection of clenobuterol hydrochloride through colloidal gold fusion latex method, and preparation methods thereof
A technology for semi-quantitative detection of clenbuterol hydrochloride, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of uneven color development, low reagent cost, slow color development, etc. Accurate effect
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Embodiment 1
[0065] A kind of detection reagent of the semi-quantitative detection clenbuterol hydrochloride of colloidal finance and latex method, the preparation method of described reagent comprises the following steps:
[0066] (1) Preparation of colloidal gold
[0067] Take 100mL of chloroauric acid aqueous solution with a mass fraction of 0.01%, heat it to boiling, add 2mL of trisodium citrate with a mass fraction of 1% under stirring, continue to boil for 15 min, and dilute to 100mL with distilled water after cooling;
[0068] (2) Preparation and purification of colloidal gold markers
[0069] Take 100 mL of the colloidal gold prepared in the above step (1) and adjust the pH to 8.0 with 0.1 mol / L potassium carbonate solution; add 1.5 mg of anti-clenbuterol monoclonal antibody while stirring, stir for 20 min, then add 25 mg dropwise mg / ml polyethylene glycol 20000 (PEG20000) 2ml, stirred for 15min; centrifuged at 20000r / min for 15min, discarded the supernatant, added 10ml of pH7.4 P...
Embodiment 2
[0079] A clenbuterol hydrochloride detection reagent strip, the reagent strip comprises the nitrocellulose membrane detection reagent prepared in the above embodiment 1, and the preparation method of the reagent strip is as follows:
[0080] (1) Paste the gel nitrocellulose membrane detection reagent prepared by the method in Example 1 above in the middle of the supporting plastic plate;
[0081] (2) Paste an absorbent pad (whatman company) above the position where the nitrocellulose membrane is fixed on the supporting plastic plate, and the absorbent pad covers the edge of the nitrocellulose membrane by 0.5-1.5 mm;
[0082] (3) Paste the glass fiber sample pad marked with colloidal gold-antibody conjugate and the polyester film marked with latex-antibody conjugate prepared in step (4) of Example 1 above under the nitrocellulose membrane; Paste the glass fiber sample pad below the polyester film to cover the polyester film by 0.5-1.5 mm;
[0083] (4) After flattening the prod...
Embodiment 3
[0086] Such as Figure 1-5 As shown, a clenbuterol hydrochloride detection kit includes a test card 1 and a test strip; the test strip is divided into a handle area 9, an observation area 10, a marking area and a sample adding area 15 from top to bottom;
[0087]The bottom plate 8 of the observation area 10 is provided with a nitrocellulose membrane, and one end of the nitrocellulose membrane near the handle area is provided with a control line 104 coated with a goat anti-mouse antibody, and the other end is provided with a first CL-BSA conjugate in turn. A detection line 101 , a second detection line 102 , and a third detection line 103 .
[0088] The test card 1 includes a card cover 11 and a box-shaped card body 12. One end of the card cover 11 is provided with a funnel-shaped sample injection hole 2 for dripping test samples, and the middle part of the card cover 11 is provided with a hole for observing the test results. The strip-shaped observation hole 3, the side corre...
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