Micro-satellite DNA (Deoxyribose Nucleic Acid) method for identifying reproductive mode of termite nest group
A technology of microsatellites and microsatellite sites, which is applied in the field of microsatellite DNA to identify the reproductive mode of termite nests, can solve the problems of not knowing the biological characteristics, and achieve the effect of improving pertinence
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[0016] The invention consists of seven key steps including sample collection, genome DNA extraction, microsatellite site search, primer design and optimization, genotype determination, chi-square test and reproductive mode determination.
[0017] The present invention is described in detail below through specific examples.
[0018] Sample collection: collect termite samples from 10 nest colonies of S. blackbreast termites in Changsha, collect 25 worker ants for each nest colony, and the distance between two sampling points should be more than 20m. After sampling, put worker ants into sample tubes, soak them in 95% alcohol, label them, and store them in a -20°C refrigerator for later use. During the sampling process, the longitude, latitude and altitude of each sampling point were measured with a handheld GPS locator.
[0019] Genomic DNA extraction: extract the genomic DNA of all samples according to the method of the existing animal tissue genomic DNA extraction kit. Use ND...
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