Phosphate solubilizing bacterium, as well as preparation method and application thereof

A technology of phosphorus-dissolving bacteria and seeds, applied in the field of microorganisms, can solve the problems of soil microbial imbalance, increasing utilization rate, and decreasing organic matter content.

Active Publication Date: 2012-08-01
MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The development of modern agriculture is accompanied by the extensive use of chemical fertilizers. While increasing production and income, it also leads to soil compaction, a decrease in organic matter content, an imbalance of microorganisms in the soil, soil acidification, and an increase in heavy metal content. At the same time, the loss of fertilizers will also cause water pollution.
At present, there is no literature report and application of phosphate-dissolving bacteria that can be used in agricultural microbial agent series fertilizers. Agricultural microbial agent series fertilizers can not only effectively improve soil quality, but also enhance crop resistance and increase crop utilization of fertilizers. rate, reduce environmental pollution, and adjust the microbial balance in the soil

Method used

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  • Phosphate solubilizing bacterium, as well as preparation method and application thereof
  • Phosphate solubilizing bacterium, as well as preparation method and application thereof
  • Phosphate solubilizing bacterium, as well as preparation method and application thereof

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Embodiment Construction

[0067] The phosphate-dissolving bacteria of the present invention are prepared by the following method:

[0068] 1) Cultivation of fermented seeds: Select the pure and excellent target bacteria cultivated in the laboratory to inoculate the seed tank, and carry out shaking culture or stirring culture for 4-5 days after inoculation, and the concentration of the bacteria is determined to reach 10 10 Above; the medium is glucose 5-15g, ammonium sulfate 0.2g (or 0.8g), calcium phosphate 6g (or 12g), sodium chloride 0.1g (or 0.5g), potassium chloride 0.1g (or 0.5g) , Magnesium sulfate 0..1 g (or 0.5g), ferrous sulfate 0.01 g (or 0.05g), manganese sulfate 0.01 g (or 0.05g), distilled water 1000ml, pH7.0 (or 7.5);

[0069] 2) Sterilization of the fermenter and medium: first sterilize the fermenter, add the medium after cooling, and then sterilize the medium. The medium is the same as the above-mentioned seed medium, steam sterilized, the pressure is controlled at 0.1-0.15Mpa, the tem...

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Abstract

The invention provides phosphate solubilizing bacterium, as well as a preparation method and application thereof. The phosphate solubilizing bacterium is Aspergillusforetidus with the preservation number of CGMCC (China General Microbiology Culture Center) No.5857. The phosphate solubilizing bacterium is used for producing a microbial fertilizer, and culturing tobacco leaves, and releases nutrition which is hardly absorbed in the soil through direct actions of solubilizing phosphor, dissolving potassium and other functional microbes, so that the soil quality is improved, the reproduction of beneficial microorganisms in the soil is promoted, the number of beneficial microorganisms in the soil is increased, the nutrient balance in the soil is regulated, balanced absorption of tobacco plants to mineral nutrition is improved, the tobacco leaf quality and fertilizer utilization are improved, and environmental pollution is reduced.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a phosphate-dissolving bacterium and its preparation method and application. Background technique [0002] The development of modern agriculture is accompanied by the extensive use of chemical fertilizers. While increasing production and income, it also leads to soil compaction, a decrease in organic matter content, an imbalance of microorganisms in the soil, soil acidification, and an increase in heavy metal content. At the same time, the loss of fertilizers can also cause water pollution. At present, there is no literature report and application of phosphate-dissolving bacteria that can be used in agricultural microbial agent series fertilizers. Agricultural microbial agent series fertilizers can not only effectively improve soil quality, but also enhance crop resistance and increase crop utilization of fertilizers. rate, reduce environmental pollution, and adjust the mi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05F11/08C12R1/66
Inventor 杨发祥尚海丽张琪
Owner MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
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