Phosphate solubilizing bacterium, as well as preparation method and application thereof
A technology of phosphorus-dissolving bacteria and seeds, applied in the field of microorganisms, can solve the problems of soil microbial imbalance, increasing utilization rate, and decreasing organic matter content.
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[0067] The phosphate-dissolving bacteria of the present invention are prepared by the following method:
[0068] 1) Cultivation of fermented seeds: Select the pure and excellent target bacteria cultivated in the laboratory to inoculate the seed tank, and carry out shaking culture or stirring culture for 4-5 days after inoculation, and the concentration of the bacteria is determined to reach 10 10 Above; the medium is glucose 5-15g, ammonium sulfate 0.2g (or 0.8g), calcium phosphate 6g (or 12g), sodium chloride 0.1g (or 0.5g), potassium chloride 0.1g (or 0.5g) , Magnesium sulfate 0..1 g (or 0.5g), ferrous sulfate 0.01 g (or 0.05g), manganese sulfate 0.01 g (or 0.05g), distilled water 1000ml, pH7.0 (or 7.5);
[0069] 2) Sterilization of the fermenter and medium: first sterilize the fermenter, add the medium after cooling, and then sterilize the medium. The medium is the same as the above-mentioned seed medium, steam sterilized, the pressure is controlled at 0.1-0.15Mpa, the tem...
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