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A method for collecting and processing pine wood nematode sex attractant

A technology of pine wood nematode and processing method, applied in the directions of attracting pests, botanical equipment and methods, biocides, etc.

Active Publication Date: 2016-08-03
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For pine wood nematode, there are no relevant reports at home and abroad on the chemical components, chemical structure, chemical properties and release amount of the sex pheromone of pine wood nematode, and how it affects its mating behavior remains to be further studied

Method used

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  • A method for collecting and processing pine wood nematode sex attractant
  • A method for collecting and processing pine wood nematode sex attractant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] When the virgin female pine wood nematode has obvious courtship calling behavior, move it to an environment with a room temperature of 25°C, put the microextraction needle close to the vulva of the female worm that is calling for mate, and then push out the needle to make it as close to the female as possible. The sex pheromones released by the female glands were continuously collected for 2 hours. Immediately after taking it out, add 1 μL of C18, C20, C21 and C22 n-alkane mixture standard dropwise to the extraction head containing sex pheromone, and then perform thermal analysis and desorption in the GC injector for 3 minutes, and the temperature of the inlet of the chromatograph is 250 °C, and finally the obtained substance (pine xylophilus sex attractant) was dropped onto the lure made of absorbent cotton, each lure contained 600 μg of pine wood nematode sex attractant, placed in the place where the pine wood nematode was raised, and the experiment was repeated three ...

Embodiment 2

[0024] The pine wood nematode sex attractant prepared by the method of Example 1 is made into lures with absorbent cotton, each lure contains 600 μg of pine wood nematode sex attractant, and then the lures are placed in the pine forest test monitoring point of insect situation monitoring, each 3 repetitions of lures and 1 control without lures, there is at least 10m of separation between each treatment, and no drugs are applied during this period. The test was continuously monitored for 10 days. After the experiment, the number of trapped pine xylophilus was counted. The test results are shown in the table below.

[0025] Table 2 embodiment is to the field control result of pine xylophilus male adult

[0026]

[0027] The test results in Table 2 show that: the embodiment has good control results for the field calling and trapping of male adults of pine xylophilus, and the effect of the lure is stable and the duration of effect is long.

[0028] In Example 1: the collecti...

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Abstract

The invention discloses a method for collecting and processing a sex attractant of pine wood nematode, which belongs to the technical field of insect control. It is characterized in that it includes the following steps: 1) selecting virgin females under a microscope; 2) using solid-phase microextraction needles to continuously collect the sex pheromones released by the female glands for 2-4 hours, and immediately dropwise add n-alkane mixed standard 0.8 ‑1.2µL, then thermal analysis and desorption in the GC injector for 2‑5min, the temperature of the inlet of the chromatograph is 230‑260°C, prepare the obtained substance into a n-hexane solution, and store it in a refrigerator at ‑20°C. The method for collecting and processing the sex attractant of pine wood nematode above is safe, reliable, simple and easy to use, and has a good effect of calling and trapping male adults of pine wood nematode, and can interfere with the mating behavior of pine wood nematode. It is safe to use and does not cause any pollution to the environment.

Description

technical field [0001] The invention belongs to the technical field of insect control, in particular to a method for collecting and processing pine wood nematode sex attractant. Background technique [0002] Pine wood nematode is a worldwide quarantine disease, which occurs seriously in my country. The rapid reproduction of nematodes in the tree is an important reason for the serious occurrence of the disease, and the efficient and frequent mating behavior is the basis for the rapid reproduction of pine xylophilus. At present, although a large number of studies on pine xylophilus have been carried out at home and abroad, the research on its behavior is quite rare. Most of the research is only a simple description of its behavior, and there is no research on the law of behavior itself, related molecular genetics, etc. Carry out in-depth and systematic research on influencing factors such as mechanism and physical and chemical conditions. [0003] The infestation behavior of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A01M99/00A01N63/02A01P19/00
Inventor 胡加付郭恺黄小娟白丽群
Owner ZHEJIANG FORESTRY UNIVERSITY
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