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Milk allergen test plate and preparation method thereof

A milk allergy and detection board technology, which is applied to measurement devices, biological tests, material inspection products, etc., can solve problems such as inability to choose, and achieve the effects of saving medical costs, simplifying routine operation processes, and intuitive and reliable results.

Active Publication Date: 2014-08-13
江苏迈源生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The purpose of the present invention is to solve the problem that in the screening process of ingestible allergens, the selection cannot be made according to the prompts of the clinician, and the detection can only be carried out according to the fixed combination method provided by the commercial kit, and IgG antibodies cannot be detected, which is easy to miss diagnosis. As well as enzyme immunoblotting, complex operation process and long reaction time, etc., a rapid milk allergen detection plate and its preparation method are provided

Method used

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  • Milk allergen test plate and preparation method thereof
  • Milk allergen test plate and preparation method thereof

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Embodiment Construction

[0031] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0032] 1. Key raw materials

[0033] (1) Milk allergens: three pure allergens, casein, β-lactoglobulin, and α-lactalbumin, were all commercial reagents and were purchased from Sigma, USA.

[0034] (2) Nitrocellulose membrane (NC): commercial material, imported and repackaged, purchased from Beyotime Company;

[0035] (3) Anti-β-lactoglobulin antibody: purchased from Abcam Company.

[0036] ⑷ Colloidal gold particles: commercial reagent, purchased from Shanghai Haoran Biotechnology Co., Ltd., imported original, item number G1527

[0037] 2. Preparation of Milk Allergen

[0038] According to literature reports, milk mainly includes three allergen components of casein, β-lactoglobulin, and α-lactalbumin. Therefore, the present technology invention selects casein, β-lactoglobulin, and α-lactalbumin to be mixed in equal concentration ratios as the Know...

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Abstract

The invention discloses a milk allergen test plate, which belongs to the gold immunochromatography detection. According to the milk allergen test plate disclosed by the invention, two ends of a PVC (polrvinyl chloride) base plate are respectively provided with a to-be-tested sample zone and an absorption zone. Colloidal gold mark antigens prepared by respectively marking colloidal gold into casein, beta lactoglobulin and alpha lactalbumin and mixing are orderly arranged between the sample zone to be tested and the absorption zone, and a nitrocellulose membrane is respectively provided with a detection zone coated with mixed milk allergen and a quality control zone coated with anti-beta lactoglobulin antibodies. In detection, color lines are formed in the detection zone and the quality contol zone when an immune complex is formed by specific milk antibodies contained in samples. If the samples do not contain specific milk antibodies, the detection zone does not display color, and only one color line is formed in the quality control zone. The milk allergen test plate disclosed by the invention with the design has the advantages of strong pertinence to the allergen detection, simplicity in operation, low cost and high sensitivity and the like, and can prevent the phenomenon of missed diagnosis in the single antibody detection. The milk allergen test plate is applied for rapidly screening patient allergic to milk, and is especially suitable for being used by primary medical treatment units.

Description

technical field [0001] The invention relates to a double-antigen sandwich gold immunochromatography detection technology, in particular to a milk allergen detection plate and a preparation method thereof. Background technique [0002] Allergic diseases refer to the body's wrong immune response to external stimuli (food, drugs, pollen, etc.), leading to various clinical symptoms. The immune response is a complex reaction process of the body after the antigen activates the immune cells; the antigen (protein) that causes the allergic reaction is called an allergen. Food allergic diseases are allergic gastroenteritis or skin symptoms caused by eating certain foods, sometimes leading to a severe systemic reaction - anaphylactic shock. Milk is an important source of nutrition for infants and even adults, and patients who are allergic to milk will have gastrointestinal symptoms and affect the healthy development of children. Milk allergy accounts for a considerable proportion of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/558
Inventor 李会强常艳敏李廼昶任杰孙辉
Owner 江苏迈源生物科技有限公司
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