Efficient culture method of tree type honeysuckle
A cultivation method and technology of honeysuckle, applied in the fields of botanical equipment and methods, horticulture, application, etc., can solve the problems of high flowering frequency, low flower yield, uneven flowering, etc., and achieve scientific and reasonable fertilizer ratio, technical system system, Reduce the effect of large leaves and flowers
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Embodiment 1
[0022] Embodiment 1 cultivation test
[0023] 1. Variety selection: Select Mengjin No. 1 suitable for planting in northern Jiangsu for cultivation experiments.
[0024] 2. Site selection and land preparation: The base of Hongshiya Ecological Agriculture Development Co., Ltd. in Donghai County, Lianyungang City, Jiangsu Province was selected. The local meteorological conditions: it is a warm temperate humid monsoon climate, with sufficient sunshine, rain and heat in the same season, four distinct seasons, and a frost-free period of 225 days throughout the year. The annual average sunshine is 2394 hours, and the annual average precipitation is 913 mm. Suitable for honeysuckle planting, choose sandy loam soil with thick humus layer, loose soil, and convenient irrigation and drainage to establish plantations. Plow 40cm deep, finely rake and flatten, and make furrows.
[0025] 3. Apply basal fertilizer: Dig a pit with a depth of 30cm according to the row spacing of 2×2m, and appl...
Embodiment 2
[0032] The mensuration of chlorogenic acid content in the honeysuckle of embodiment 2
[0033] Accurately weigh 0.5 g of Mengjin No. 1 honeysuckle sample (through No. 4 sieve), put it in a stoppered Erlenmeyer flask, accurately add 50 ml of 50% methanol, weigh it, ultrasonically treat it for 30 minutes, let it cool, and weigh it again. Use 50% methanol to make up for the lost weight, shake well, filter, accurately measure 5ml of the filtrate, put it in a 25ml brown measuring bottle, add 50% methanol to the mark, and set aside.
[0034] Accurately weigh 0.0041 g of the chlorogenic acid reference substance into a 100 ml brown volumetric flask, add 50% methanol to dissolve to constant volume, and obtain a reference substance solution with a concentration of 41 μg / ml.
[0035] Accurately draw 5 μl each of the reference substance solution and the test solution, inject it into the liquid chromatograph, and measure it. The chromatographic conditions are: determined by methodology re...
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