High-performance liquid chromatography determination method of enramycin

Abstract

The invention provides a high-performance liquid chromatography determination method of enramycin, comprising the following steps of: preparing a buffering salt solution; processing a sample; processing a standard product; feeding the sample; collecting a map and recording a peak area; processing data; calculating area and the like. The high-performance liquid chromatography determination method of the enramycin has the advantages that: 1: the sample processing is simple; 2: a detection step is simple and has low requirements on staff; and the method mainly depends on instrument analysis, simplicity and easiness of learning and easiness of popularization; 3: a detection period is short: the time for feeding and injecting the sample is 30 minutes and a result can be represented in the day of detecting; and 4: the relative deviation of the detected result is small: the method requires that the relative deviation of results of two times of parallel determination on the same sample by the same operator is not more than 2%.

Description

technical field [0001] The invention relates to a high performance liquid chromatography determination method for enramycin in compound feed, concentrated feed, premixed feed and enramycin premix. Background technique [0002] Enramycin (enramycin) is fermented by actinomyces (Streptomyces fungicidicus). It is a polypeptide antibiotic combined with unsaturated fatty acids and more than a dozen amino acids. The main components are enramycin A and B, which are its hydrochloride form application. White or slightly yellowish white powder (the crude product is gray or taupe powder with special odor). Decompose at 234~238℃, easily soluble in dilute hydrochloric acid, slightly soluble in water, methanol, ethanol, insoluble in acetone. Enramycin has strong activity against Gram-positive bacteria, especially against harmful Clostridium in the intestine. It is not easy to produce drug resistance after long-term use, because it changes the bacterial population in the intestine, so i...

AI Technical Summary

Problems solved by technology

There are several deficiencies in this method in the detection process: 1, sample processing is loaded down with trivial details, this method first uses acidic methanol solution to extract enramycin, then the extract is adsorbed and eluted with macroporous resin; 2, detection operation The steps are complex and have high requirements for testing personnel. The testing steps of this method include preparing flat plates, placing Oxford cups, adding samples, and culturing. There are many key links in which testing personnel are required to have rich experience and skilled techniques; 3. Testing cycle Long, this method needs to be incubated at 36°C-37°C for 16h-18h, and it cannot be detected on the day of the test; 4. The relative deviation of the test results is large, and this method requires the same operator to perform two parallel measurements on the same sample at the same time. The relative deviation is not more than 15%.