Ziziphora clinopodioides Lam fingerprint and establishment method thereof

A technique for fingerprinting and establishing methods, which is applied in the field of drug analysis, can solve problems such as difficult to achieve effective quality control of lip vanilla, insufficient quality stability, etc., and achieve high sensitivity and stable quality

Inactive Publication Date: 2015-03-04
XINJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is not enough to check the stability of the quality only by measuring a certain active ingredient, and it is difficult to achieve effective control of the quality of lip vanilla

Method used

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  • Ziziphora clinopodioides Lam fingerprint and establishment method thereof
  • Ziziphora clinopodioides Lam fingerprint and establishment method thereof
  • Ziziphora clinopodioides Lam fingerprint and establishment method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Source of medicinal materials:

[0033] Sources and similarity of 10 samples of lip vanilla from different origins

[0034]

[0035] Lip vanilla was identified as Ziziphora clinopodioides Lam. by Li Yonghe, chief pharmacist of the Department of Traditional Chinese Medicine, College of Traditional Chinese Medicine, Xinjiang Medical University.

[0036] 1. Preparation of standard solution

[0037] The standard reference substances used in this method are diosmin, mongoside, pulegone

[0038] Weighed an appropriate amount of the standard substance and dissolved them in methanol. It was found that pulegone could be well dissolved in methanol, while diosmin and mongoside had very little solubility in methanol and were almost insoluble. So add co-solvent DMSO to help it dissolve. Standards were prepared in 25% v / v DMSO in methanol. The solutions with concentrations of 0.122 mg / ml, 0.276 mg / ml, and 0.063 mg / ml of diosmin, mongoside and pulegone, respectively, were us...

Embodiment 2

[0058] (1) Preparation of standard solution:

[0059] Weigh the standard products of diosmin, montanoside and pulegone respectively, dissolve them in a methanol solution containing 20% ​​v / v of dimethyl sulfoxide and settle to volume, and make diosmin, montanoside and pulegone Menthone concentrations of 0.122mg / ml, 0.276mg / ml, and 0.063mg / ml were used as standard solutions.

[0060] (2) Preparation of the test solution:

[0061] Accurately weigh 1.000g of lip vanilla coarse powder from different origins, put it into a 30mL conical flask with a stopper, use 8mL of 20% DMSO methanol as solvent, soak for 0.5h, ultrasonically extract for 30min, filter, wash with 20% DMSO methanol and dilute to 10mL Put it in a volumetric flask, shake well, pass through a 0.22μm microporous membrane, and obtain.

[0062] All the other operations are the same as in Example 1.

Embodiment 3

[0064] (1) Preparation of standard solution:

[0065] Weigh the standard products of diosmin, montanoside and pulegone respectively, dissolve them with 30% v / v methanol solution containing dimethyl sulfoxide and make the diosmin, montanoside and pulegone Menthone concentrations of 0.122mg / ml, 0.276mg / ml, and 0.063mg / ml were used as standard solutions.

[0066] (2) Preparation of the test solution:

[0067] Accurately weigh 1.000g of lip vanilla coarse powder from different origins, put it into a 30mL conical flask with a stopper, use 15mL of 30% DMSO methanol as solvent, soak for 1.5h, ultrasonically extract for 1h, filter, wash with 30% DMSO methanol and dilute to 10mL Put it in a volumetric flask, shake well, pass through a 0.22μm microporous membrane, and obtain.

[0068] All the other operations are the same as in Example 1.

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Abstract

The present invention provides a Ziziphora clinopodioides Lam fingerprint establishment method and a Ziziphora clinopodioides Lam fingerprint obtained thereby, including the following steps: (1) standard solution preparation: weighing standards, such as, diosmin, linarin and pulegone, dissolving the standards by using a methanol solution containing dimethyl sulfoxide and performing volume metering to obtain a standard solution; (2) test solution preparation: using the methanol solution containing dimethyl sulfoxide to immerse Ziziphora clinopodioides Lam powder, and performing ultrasonic extraction, filtration, volume metering and microfiltration membrane filtration to obtain a test solution; and (3) RRLC analysis: performing gradient elute with an XDB-C18 chromatographic column according to the following conditions to obtain a Ziziphora clinopodioides Lam HPLC fingerprint, wherein the column volume is 50 mm*4.6 mm*1.8 mm, flow rate is 0.9 ml / min, a detection wavelength is 270 nm, a column temperature is 30 DEG C, a sample volume is 6 ml and a mobile phase is an acetic acid solution-methanol. Based on existence and non-existence of the common peak in the fingerprint provided in the invention, the quality of Ziziphora clinopodioides Lam can be effectively monitored, stable, uniform and controllable quality being guaranteed.

Description

technical field [0001] The invention relates to a method for establishing a lip herb fingerprint spectrum and the fingerprint spectrum, belonging to the technical field of drug analysis. Background technique [0002] At present, fingerprinting has become an internationally recognized effective means to control the quality of traditional Chinese medicine or natural medicine, and fingerprinting technology has become a hot issue in the field of traditional Chinese medicine. Internationally, countries that use fingerprint quality control technology to control the quality of botanical drugs include South Korea, Japan, Germany, etc. For example, Germany uses fingerprint technology to control the quality of ginkgo preparations; the US FDA requires the quality control of botanical drugs to control fingerprints. testing standards. The use of fingerprints to formulate quality standards for injections has also been included in the Pharmacopoeia, but in my country's existing standards,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 田树革周晓英哈木拉提·吾甫尔于谦刘宏炳葛亮
Owner XINJIANG MEDICAL UNIV
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