Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Heat-resisting freeze-dried protective agent for live vaccine for chicken infectious bursal diseases and preparation method for heat-resisting freeze-dried protective agent

A heat-resistant freeze-drying and bursal disease technology, applied in antiviral agents, pharmaceutical formulas, viral antigen components, etc., can solve the problems of reducing production costs and achieve the effect of low virus loss rate and high aging resistance

Inactive Publication Date: 2013-07-03
QINGDAO AGRI UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The invention provides a heat-resistant freeze-drying protective agent for chicken infectious bursal disease live vaccine. Technical problems of long-term storage and transportation at 2°C to 8°C, and simplify the preparation method to reduce production costs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Preparation of Heat-resistant Freeze-dried Vaccine of Chicken Infectious Bursal Disease Live Vaccine

[0021] 1) Mix carboxymethyl cellulose sodium with water for injection, heat at 98-100°C for 10-20 minutes, cool to room temperature, and prepare a concentration of 6% (the concentration involved in the present invention is all unless otherwise specified. is the mass percent concentration) of liquid A.

[0022] 2) Mix xylooligosaccharides, sorbitol and water for injection to prepare liquid B with a concentration of 9% xylooligosaccharides.

[0023] 3) Mix polyvinylpyrrolidone, sodium glutamate, sodium selenite, and potassium chloride with water for injection, stir and mix well, and prepare liquid C with 6% polyvinylpyrrolidone and 2.4% sodium glutamate , Sodium selenite concentration 1.2%, potassium chloride 0.03%.

[0024] 4) Prepare liquid A, liquid B, and liquid C at a volume ratio of 1:1:1 to make a vaccine freeze-drying protective agent.

[0025] 5) Mix the vacc...

Embodiment ２

[0029] Preparation of Heat-resistant Freeze-dried Vaccine of Chicken Infectious Bursal Disease Live Vaccine

[0030] 1) Mix carboxymethylcellulose sodium with water for injection, heat at 98-100°C for 10-20 minutes, cool to room temperature, and prepare liquid A with a concentration of 9%.

[0031] 2) Mix xylooligosaccharides, sorbitol and water for injection to prepare liquid B, with a concentration of xylooligosaccharides of 12%.

[0032] 3) Mix polyvinylpyrrolidone, sodium glutamate, sodium selenite, and potassium chloride with water for injection, stir and mix well, and prepare liquid C with 6% polyvinylpyrrolidone and 2.4% sodium glutamate , Sodium selenite concentration 1.5%, potassium chloride 0.1%.

[0033] 4) Prepare liquid A, liquid B, and liquid C at a volume ratio of 1:1:1 to make a vaccine freeze-drying protective agent.

[0034] 5) Mix the vaccine lyoprotectant with the virus liquid of chicken infectious bursal disease live vaccine at a volume ratio of 4:1.

...

Embodiment ３

[0038] Preparation of Heat-resistant Freeze-dried Vaccine of Chicken Infectious Bursal Disease Live Vaccine

[0039] 1) Mix carboxymethylcellulose sodium with water for injection, heat at 98-100°C for 10-20 minutes, cool to room temperature, and prepare liquid A with a concentration of 9%.

[0040] 2) Mix xylooligosaccharides, sorbitol and water for injection to prepare liquid B, with a concentration of xylooligosaccharides of 18%.

[0041] 3) Mix polyvinylpyrrolidone, sodium glutamate, sodium selenite, potassium chloride with water for injection, stir and mix, and prepare liquid C, polyvinylpyrrolidone 6%, sodium glutamate concentration 1.8%, sub Sodium selenate concentration 1.2%, potassium chloride 0.15%.

[0042] 4) Prepare liquid A, liquid B, and liquid C at a volume ratio of 1:1:1 to make a vaccine freeze-drying protective agent.

[0043] 5) Mix the vaccine lyoprotectant with the virus liquid of chicken infectious bursal disease live vaccine at a volume ratio of 5:1. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a heat-resisting freeze-dried protective agent for a live vaccine for chicken infectious bursal diseases and a preparation method for the heat-resisting freeze-dried protective agent. The heat-resisting freeze-dried protective agent for the live vaccine for chicken infectious bursal diseases is prepared from the following components in percentage by weight: 2 to 3 percentof sodium carboxy methyl cellulose, 3 to 6 percent of xylo-oligosaccharide, 2 to 3 percent of polyvinylpyrrolidone, 0.5 to 0.8 percent of glutamine, 0.1 to 0.5 percent of sodium selenite, 0.01 to 0.05 percent of potassium chloride and the balance of water for injection. By the heat-resisting freeze-dried protective agent, the technical problem that the live vaccine for chicken infectious bursal diseases cannot be stored and transported for long time at the temperature of between 2 and 8 DEG C is solved, production process is simplified, and production cost is reduced.

Description

Technical field [0001] The invention involves a chicken infectious method, the heating and freeze -resistant protective agent, and its preparation methods, which belong to the field of animal vaccine production. Background technique [0002] Frozen drying technology is an important application technology of biology. It is commonly used in scientific research and production of biological products. It maintains the biological characteristics of bacteria and poisonous species, stabilizes the biological activity of biological raw materials and semi -finished products, and extends the validity period of the finished product.The frozen -dried protective agent is a key technology in the production of live vaccines, which has a great impact on the preservation, transportation and use of vaccines. [0003] Foreign vaccine frozen and dry products are stored at 2 to 8 ° C for more than 15 months, but the protective agent formula is strictly confidential. In my country's animal vaccine, livi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/38A61K47/26A61K47/04A61K39/12A61P31/14
Inventor 郝智慧曲宝涵苑国春丁兆鹏杨芬芳
Owner QINGDAO AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com