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Newcastle disease and avian infectious bronchitis bigeminal live vaccine heat-resisting freeze-drying protective agent and preparation method

A technology for chicken contagiousness and bronchitis, which is used in antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problems of long-term storage and transportation, etc., and achieves high aging resistance and low virus loss rate. Effect

Inactive Publication Date: 2013-06-05
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The invention provides a heat-resistant freeze-drying protectant for chicken Newcastle disease and chicken infectious bronchitis dual live vaccine. The only technical problem is that the vaccine cannot be stored and transported at 2°C to 8°C for a long time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation of heat-resistant and freeze-dried vaccine of chicken Newcastle disease and chicken infectious bronchitis dual live vaccine

[0020] 1) Mix sodium carboxymethylcellulose with water for injection, heat at 98-100°C for 10-20 minutes, cool to room temperature, and prepare a concentration of 3% (the concentration involved in the present invention is all unless otherwise specified. is the mass percent concentration) of liquid A.

[0021] 2) Mix xylo-oligosaccharide, sorbitol and water for injection to prepare liquid B, the concentration of xylo-oligosaccharide is 8%, and the concentration of sorbitol is 3%.

[0022] 3) Mix sodium glutamate, sodium selenite, and water for injection, stir and mix well, and prepare liquid C with a concentration of sodium glutamate of 1.5% and a concentration of sodium selenite of 1.5%.

[0023] 4) Prepare liquid A, liquid B, and liquid C at a volume ratio of 1:1:1 to make a vaccine freeze-drying protective agent.

[0024] 5) Mix t...

Embodiment ２

[0028] Preparation of heat-resistant and freeze-dried vaccine of chicken Newcastle disease and chicken infectious bronchitis dual live vaccine

[0029] 1) Mix carboxymethylcellulose sodium with water for injection, heat at 98-100°C for 10-20 minutes, cool to room temperature, and prepare liquid A with a concentration of 3%.

[0030] 2) Mix xylo-oligosaccharides, sorbitol and water for injection to prepare liquid B, the concentration of xylo-oligosaccharides is 6%, and the concentration of sorbitol is 3%.

[0031] 3) Mix sodium glutamate, sodium selenite, and water for injection, stir and mix well, and prepare liquid C with a concentration of sodium glutamate of 1.5% and a concentration of sodium selenite of 0.3%.

[0032] 4) Prepare liquid A, liquid B, and liquid C at a volume ratio of 1:1:1 to make a vaccine freeze-drying protective agent.

[0033] 5) Mix the lyoprotectant of the vaccine with the virus solution of the Newcastle disease and infectious bronchitis dual live vac...

Embodiment ３

[0037] Preparation of heat-resistant and freeze-dried vaccine of chicken Newcastle disease and chicken infectious bronchitis dual live vaccine

[0038] 1) Mix carboxymethylcellulose sodium with water for injection, heat at 98-100°C for 10-20 minutes, cool to room temperature, and prepare liquid A with a concentration of 6%.

[0039] 2) Mix xylo-oligosaccharides, sorbitol and water for injection to prepare liquid B, the concentration of xylo-oligosaccharides is 10%, and the concentration of sorbitol is 6%.

[0040] 3) Mix sodium glutamate, sodium selenite, and water for injection, stir and mix well, and prepare liquid C with a concentration of sodium glutamate of 1.5% and a concentration of sodium selenite of 0.3%.

[0041] 4) Prepare liquid A, liquid B, and liquid C at a volume ratio of 1:1:1 to make a vaccine freeze-drying protective agent.

[0042] 5) Mix the vaccine lyoprotectant with the virus solution of the Newcastle disease and infectious bronchitis dual live vaccine a...

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Abstract

The invention relates to a newcastle disease and avian infectious bronchitis bigeminal live vaccine heat-resisting freeze-drying protective agent and a preparation method. The newcastle disease and avian infectious bronchitis bigeminal live vaccine heat-resisting freeze-drying protective agent provided by the invention comprises the following components: 1-2% of sodium carboxymethylcellulose, 2-8% of xylo-oligosaccharide, 1-3% of sorbitol, 0.5-0.8% of sodium glutamate, 0.1-0.5% of sodium selenite and the balance of water for injection. The heat-resisting freeze-drying protective agent provided by the invention solves the problem that the newcastle disease and avian infectious bronchitis bigeminal live vaccine can not be stored for a long time and transported at 2-8 DEG C.

Description

technical field [0001] The invention relates to a heat-resistant freeze-drying protectant for chicken Newcastle disease and chicken infectious bronchitis dual live vaccine, and a preparation method thereof, belonging to the field of animal vaccine production. Background technique [0002] Freeze-drying technology is an important application technology in biology. It is often used in the scientific research and production of biological products to maintain the biological characteristics of bacteria and viruses, stabilize the biological activity of biological raw materials and semi-finished products, and extend the validity period of finished products. Lyoprotectant is a key technology in the production of live vaccines, which has a great impact on the storage, transportation and use of vaccines. [0003] The freeze-dried products of foreign live vaccines have a shelf life of more than 15 months at 2-8°C, but the formula of the protective agent is strictly confidential. Among ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/38A61K39/295A61P31/14A61K39/17A61K39/215
Inventor 郝智慧曲宝涵苑国春丁兆鹏杨芬芳
Owner QINGDAO AGRI UNIV
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