Compound bacterium agent capable of decomposing cotton stalks quickly and preparation method thereof

A compound bacterial agent and compound bacteria technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of weak decomposition ability, difficult cultivation, and long fermentation cycle of a single bacterial species

Inactive Publication Date: 2012-09-19
FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nowadays, a single strain of bacteria is mostly used for decomposition. The decomposition ability of a single strain is weak, the adaptabi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0038] 1. Preparation of cotton stalk compost.

[0039] (1) Crushing of cotton stalks: use a professional cotton stalk grinder to crush the natural air-dried cotton stalks into small pieces of about 1-1.5 cm;

[0040] (2) Preparation of livestock and poultry manure: dry pig manure, cow manure and chicken manure in a cool and ventilated place until the water content is 40-60%;

[0041] (3) The total compost is designed to be 200 kg, and the livestock and poultry manure and cotton stalks are evenly mixed, and the dry matter mixing ratio is pig manure: cow manure: chicken manure: cotton stalk=1:0.5: 0.5: 4; The initial moisture content is 50%, and the initial carbon-nitrogen ratio is adjusted to be 30;

[0042] (4) After composting starts, in the first 7-10 days, turn the pile 2-3 times a day; in the 10-20 day, turn the pile 1-2 times a day; in the 20-30 day, turn the pile once every 2-3 days; 31-45 days, every 4-6 days, turn once every 4-6 days, 46-60 days, turn once every 7-1...

example 2

[0060] 1. Preparation of rice straw compost. The mixing ratio of dry matter is pig manure: cow manure: chicken manure: rice straw = 1.5:1:1:4.5, adjust the initial moisture content of the pile to 60%, and the rest are the same as Example 1.

[0061] 2. Screening of complex strains of rapid decomposition of cotton stalks. Timely take 30 g of compost and add it to a 500 mL Erlenmeyer flask, and add 400 mL of sterilized normal saline.

[0062] Take the flask out of the shaker and let it stand for 30 min, take 20 mL of the upper liquid as the inoculation solution and inoculate it into a 500 mL flask containing 350 mL of modified PSC medium, and place it in a constant temperature incubator at 60 °C in the dark for static culture.

[0063] The culture medium is Mandels medium.

[0064] Mandels medium composition: peptone 2.5g, (NH 4 ) 2 SO 4 1.4g, MgSO 4 ·7H 2 O 0.3g, KH 2 PO 4 2g, FeSO 4 ·7H 2 O 5mg, MnSO 4 1.6mg, ZnCl 2 1.7 mg, CoCl 2 1.7mg, CaCO 3 2 g, 2 g dr...

example 3

[0073] 1. Preparation of wheat straw compost.

[0074] The total compost is designed to be 200 kg, and the livestock and poultry manure and wheat straw are mixed evenly, and the dry matter mixing ratio is pig manure: cow manure: chicken manure: cotton stalk=2: 1.5: 1.5: 6.

[0075] All the other are the same as example one.

[0076] 2. Screening of wheat straw rapid decomposition compound strains.

[0077]The culture medium is Clostridium proliferation medium: tryptone 10.0g, yeast extract 3g, NaCl 5.0g, cysteine ​​hydrochloride 0.5g, sodium acetate 3.0g, calcium carbonate 3g, dry cotton stalk 2.5g, distilled water 1 L, pH 7.0.

[0078] The subculture was repeated 60 times until the decomposition ability was stable.

[0079] The amount of bacterial solution sprayed for the fifth time was 10% of the weight of wheat straw powder.

[0080] All the other are the same as example one.

[0081] 3. Production of compound bacterial agent for rapid decomposition of cotton stalks. ...

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PUM

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Abstract

The invention relates to a compound bacterium agent capable of decomposing cotton stalks quickly and a preparation method thereof. The compound bacterium agent comprises the following components in percent by cell content: 10-20% of clostridium thermocellum, 5-15% of desulfotomaculum, 5-35% of thermophilic bacillus licheniformis, 5-20% of butyrivibrio fibrisolvens, 25-35% of bacillus subtilis and 10-20% of lactobacillus thermophilus. The preparation method comprises the following steps of: crushing air-dried cotton stalks and the manure of pigs, cows and chicken till the water content is 40-60%, mixed stacking, mixing, stacking, turning, measuring the indexes such as temperature and cotton-stalk decomposing amount; taking compost into a triangular flask in proper time, adding physiological saline or PBS (Phosphate Buffer Solution) in a swinging table for vibration, taking the obtained mixture, standing, inoculating the bacterium solution according to the mixing quantity, putting the mixture into a culture medium for constant-temperature dark and static culture; taking the bacteria with strong decomposition as strains for next subculture repeatedly, and carrying out subculturing circularly and repeatedly for 30-60 times to obtain the stable compound bacterium system; and adding yeast powder and the like, spraying bacterium solution, and drying for multiple times to obtain the compound bacterium agent. The compound bacterium agent can realize high-efficiency decomposition for cellulose and hemicelluloses in the cotton stalks, and is used for the purposes of energy conversion, feeding and the like of the cotton stalks.

Description

technical field [0001] The invention relates to a composite bacterial agent capable of rapidly decomposing cotton stalks and a preparation method thereof. Background technique [0002] After cotton is harvested, a large amount of by-products—cotton stalks—will remain. The main component of cotton stalk is natural lignocellulose (composed of cellulose, hemicellulose and lignin, etc.), which is a lignocellulosic biomass resource with development potential. Through biodegradation and transformation, biofuels (such as ethanol, Hydrogen and biogas, etc.), animal feed, organic fertilizer, and edible fungus cultivation materials, etc. [0003] Among crop straws, cotton straw is harder and harder to decompose than rice straw, wheat straw and corn straw. At present, due to the lack of microorganisms (or enzymes) capable of efficiently decomposing cotton stalks, their biotransformation (including energy, feed, etc.) efficiency is very low, and the biological utilization of cotton st...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/145C12R1/10C12R1/63C12R1/125C12R1/225C12R1/01
Inventor 郭鹏周明崔宗均杨德高虹程薇王少华史德芳李露薛淑静陈明利张金木叶丽秀王俊
Owner FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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