Pressurized thin-layer chromatography development chamber

Abstract

The invention discloses a pressurized thin-layer chromatography development chamber which comprises a pressure bearing ring, wherein a top plate and a bottom plate are arranged in the pressure bearing ring, and the top plate is fixed at the top of the pressure bearing ring; at least two guide posts are also arranged in the pressure bearing ring; the bottom plate is penetrated in the guide posts and slides up and down, and the bottom surface of the bottom plate is located at the upper end of an ultrathin hydraulic cylinder, while the lower end of the ultrathin hydraulic cylinder is supported at the bottom of the pressure bearing ring; and the top plate is provided with a developing solvent inlet and a developing solvent outlet. According to the invention, the pressure applied to a pressurized thin-layer chromatography prefabricated plate (also known as a flat plate column) is uniform and stable, so that the developing process of a test sample on the pressurized thin-layer chromatography prefabricated plate can be guaranteed to be smoothly and reliably carried out; and the ultrathin hydraulic cylinder is used for providing pressure for the development chamber, so that the structure is greatly simplified, and the volume and the weight of the device are reduced. The pressurized thin-layer chromatography development chamber can be used for separating and preparing samples, and not only can be used for off-line detection, but also can be used for finishing on-line detection.

Description

technical field [0001] The invention relates to a plane liquid chromatographic analysis technique under pressurized conditions, also known as pressurized thin-layer chromatography. The pressurized development chamber has the function of open offline operation of planar chromatography; at the same time, it has the function of closed online operation of column chromatography. Background technique [0002] Traditional thin-layer chromatography is a commonly used method in planar chromatography. The adsorbent (stationary phase) is coated into a uniform thin layer on a carrier such as glass, plastic or aluminum foil, and after consolidation, it becomes a prefabricated thin layer chromatography. plate (thin-layer plate); place the sample to be measured on one end of the thin-layer plate, and put it into the developing chamber (glass cylinder), and the developing agent (mobile phase) at the bottom borrows the capillary action inside the adsorbent material, from the spotting point ...

AI Technical Summary

Problems solved by technology

[0010] However, the existing pressurized expansion chambers have defects such as complex structure, high cost, bulky, too heavy, etc.

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