Gating fluorescence service life imaging device based on light delay

Abstract

The invention relates to a fluorescence service life imaging device based on light delay. The fluorescence service life imaging device comprises a laser, a sample table, a beam splitter, a first coupling lens, a reflector, a second coupling lens, a first optical fiber imaging beam, a second optical fiber imaging beam, a narrow band filter, an image reinforced charge coupled device (CCD) and a computer, wherein the sample table is positioned on a light outlet light path of the laser, the beam splitter is positioned on the light outlet light path of the sample table, the first coupling lens is positioned on a reflecting light path of the beam splitter, the reflector is positioned on a transmitting light path of the beam splitter, the second coupling lens is positioned on a reflecting light path of the reflector, the first optical fiber imaging beam is positioned on a light path of the first coupling lens, the second optical fiber imaging beam is positioned on a light path of the second coupling lens, the narrow band filter is positioned on light paths of the first optical fiber imaging beam and the second optical fiber imaging beam, the image reinforced CCD is positioned at one side of the narrow band filter far away from the first optical imaging beam, and the input end of the computer is connected with the output end of the image reinforced CCD. The fluorescence service life imaging device can respectively obtain transient state or steady state fluorescence service life images of organism auto-fluorescence substances or exogenous fluorescence substances.

Description

technical field [0001] The invention belongs to the field of fluorescence lifetime imaging, in particular to a gated fluorescence lifetime imaging device based on light delay. Background technique [0002] Many substances in living organisms have autofluorescence, such as certain types of amino acids, NADPH, flavin and melanin, etc., and their lifetimes are mostly on the order of nanoseconds. Exogenous fluorescence uses fluorophores to combine with specific target molecules to achieve labeling of target molecules: the main fluorophores can be divided into the following categories: organic dyes, fluorescent proteins, quantum dots and long-lived rare earth complexes. Organic dyes such as Cy 5 , the Rhodamin series have corresponding dyes from ultraviolet to infrared bands, and their fluorescence lifetimes range from ns to tens of ns. Commonly used fluorescent proteins such as ECPF and mRFP1 have an excitation wavelength of 400-500 nm and a fluorescence lifetime of several ns...

AI Technical Summary

Problems solved by technology

Although the electrical delay device is flexible, it requires high delay accuracy, which requires high hardware performance and increases the complexity of the system.

[0005] The above patents and literatures have proposed various imaging methods for steady-state fluorescence lifetime acquisition, but none of them has solved the problem of transient fluorescence imaging.

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