Urease-producing microorganisms and method for solidifying heavy metals in foundation using same

A technology for microorganisms and urease production, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of producing toxic sludge or other wastes, incomplete removal, high cost, etc., and achieves low cost and effect. Good, short time effect

Active Publication Date: 2014-04-02
TSINGHUA UNIV
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Problems solved by technology

[0003] Traditional heavy metal treatment methods mainly include: chemical precipitation method, ion exchange method, evaporation concentration method, electrolysis method, activated carbon and silica gel adsorption method and membrane separation method, etc., but these methods have incomplete removal, high cost, generation of toxic sludge or Disadvantages such as other waste

Method used

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  • Urease-producing microorganisms and method for solidifying heavy metals in foundation using same
  • Urease-producing microorganisms and method for solidifying heavy metals in foundation using same
  • Urease-producing microorganisms and method for solidifying heavy metals in foundation using same

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Embodiment

[0032] Isolation and Screening of Strains Producing Urease with the Ability to Fix Heavy Metals

[0033] 1. Sample collection

[0034] Sporosarcina pasteurii comes from the American type culture collection (American type culture collection), numbered ATCC11859, and Terrabacter tumescens comes from the General Microbiology Center of China Microbial Culture Collection Management Committee, numbered AS.1.2690.

[0035] 2. Isolation and screening of strains producing urease

[0036] The urease-producing strain was isolated from the flower garden of Tsinghua University to collect soil samples.

[0037] Bacteria have urea decomposing enzyme, which can decompose urea to produce a large amount of ammonia, making the culture medium alkaline and red. This experiment takes advantage of this feature. The test sample is first enriched and cultured at 37°C and 5M high-concentration urea for 24 hours to kill various microbial vegetative cells that cannot tolerate and use high-concentration urea, and ...

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Abstract

Urease-producing microorganisms of Sporosarcina antarctica UR53, Sporosarcina koreensis UR47, Sporosarcina sp. UR31 and Bacillus lentus UR41 were preserved in China General Microbiological Culture Collection Center (CGMCC) on March 16, 2012 with the preservation numbers of CGMCC NO.5916, CGMCC NO.5915, CGMCC NO.5913 and CGMCC NO.5914, respectively. The method for solidifying heavy metals in the foundation by using the urease-producing microorganisms comprises the steps of: carrying out fermentation culture on the four microorganisms and microorganisms of Sporosarcina pasteuril and Terrabacter tumescens in a fermentation culture medium to obtain microorganism liquid, adding reaction liquid urea to the microorganism liquid, and then adding to the solution containing heavy metals to form a mixed solution, so that microorganism-heavy metal flocculates are formed in the mixed solution and then water-insoluble heavy metal carbonates are further formed. The method for solidifying the heavy metals in the foundation by using the microorganisms has the advantages of short solidifying time, good effect, low cost and no secondary pollution to environment.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms and their solidification of heavy metals, and specifically relates to a microorganism producing urease and a method for solidifying heavy metals in a foundation. Background technique [0002] Heavy metal pollution means that due to human production and activities, the content of heavy metals in the environment is significantly higher than its background value. Due to the long-term retention and non-degradability of heavy metal ions, it has caused great damage to the ecological environment. With the large-scale urban expansion and construction, many buildings were built near abandoned factories and garbage dumps, and heavy metal pollution in the foundations was serious. Heavy metal elements can eventually enter the organism through the food chain, destroy the normal metabolism of the organism, and seriously endanger human health. It has become an environmental problem that cannot be ignored. [00...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01C12R1/07
Inventor 程晓辉李萌郭红仙
Owner TSINGHUA UNIV
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