Analyzing and detecting method for rhizoma corydalis water-soluble non-alkaloid compound

Abstract

The invention relates to the fields of analytical chemistry and medicinal chemistry, and discloses an analyzing and detecting method for rhizoma corydalis water-soluble non-alkaloid compound. The method is characterized by including steps of firstly, taking extract of the rhizoma corydalis water-soluble non-alkaloid compound, and filtering after high speed centrifugation; secondly, drying supernatant obtained from the first step and performing trimethyl silylation derivatization treatment to the dried supernatant; thirdly, analyzing derivatization product obtained in the second step using gas chromatography-mass spectrometry. By the method, component and content of the rhizoma corydalis water-soluble non-alkaloid compound can be detected simply, fast and accurately, significance is brought to fully understanding of chemical component of traditional rhizoma corydalis and quality control of the rhizoma corydalis water-soluble extract, research data are provided for further development of the rhizoma corydalis, research and production are safer, and effective novel rhizoma corydalis with controllable quality can be available.

Description

technical field [0001] The invention relates to the fields of analytical chemistry and medicinal chemistry, in particular to a method for analyzing and detecting water-soluble non-alkaloid compounds of Corydalis Corydalis. Background technique [0002] Corydalis yanhusuo (Corydalis yanhusuo W.T.Wang), a traditional Chinese medicine, is the dry tuber of Corydalis yanhusuo W.T.Wang in the family Papaveraceae. It is mainly used for chest, hypochondrium, epigastric and abdominal pain, amenorrhea, dysmenorrhea, postpartum stasis, tumbling, swelling and pain, etc. [0003] In the screening study of the pharmacodynamic part of Corydalis Corydalis, it was found that the 80% ethanol extract of Corydalis Corydalis passed through the DA201 macroporous resin column, and the enriched pure water eluted part showed a good anti-myocardial ischemia effect in the pharmacodynamic test (large dose, 350mg / kg). Therefore, this part has certain research value, and relevant patents have been auth...

AI Technical Summary

Problems solved by technology

But it also has huge disadvantages: due to the large number of components in natural products, the existence of various large and small molecular compounds, monosaccharide isomers and special monosaccharides of different size rings, resulting in more chromatographic peaks than the number of component monosaccharides, As a result, the qualitative and quantitative analysis of the mixture is complicated, and the analysis of the active ingredients in natural products cannot be effectively completed. Therefore, this method has not been practically applied in the analysis of natural products at present.

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