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Preparation method of ofloxacin and 17beta-estradiol double-template molecularly-imprinted composite microsphere

A technology of ofloxacin and molecular imprinting, applied in chemical instruments and methods, and other chemical processes, can solve the problems of cumbersome detection, missed detection in the detection process, singleness, etc., and achieve the effect of mild reaction conditions and good recognition performance

Inactive Publication Date: 2012-10-10
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the currently synthesized molecularly imprinted polymers (MIP) use a single template molecule. When multiple drugs remain, it can only detect one of them, and cannot detect other coexisting drugs at the same time. Missed or cumbersome detection problems
There are also people who use multiple drugs of the same type as templates, or use two isomers as templat

Method used

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  • Preparation method of ofloxacin and 17beta-estradiol double-template molecularly-imprinted composite microsphere
  • Preparation method of ofloxacin and 17beta-estradiol double-template molecularly-imprinted composite microsphere
  • Preparation method of ofloxacin and 17beta-estradiol double-template molecularly-imprinted composite microsphere

Examples

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Example Embodiment

[0029] Example 1

[0030] The preparation method of ofloxacin and 17β-estradiol double-template molecularly imprinted composite microspheres in this embodiment includes the following steps:

[0031] Step 1: Synthesis of monodisperse polystyrene dispersion

[0032] Add 10.908g styrene, 0.218g azobisisobutyronitrile, 1.528g polyvinylpyrrolidone k-30 (Jiaozuo Zhongwei Chemical Co., Ltd.) with a number average molecular weight of 40,000 into a 250mL single-necked flask containing 96g of absolute ethanol, The mass ratio of styrene to azobisisobutyronitrile, polyvinylpyrrolidone, and anhydrous ethanol is 1:0.02:0.14:8.8. Use an ultrasonic cleaner for ultrasonic dispersion at a power of 80W for 20 minutes, and nitrogen gas for 15 minutes to remove oxygen. Stir, react at 70°C for 24 hours, centrifuge and wash with absolute ethanol to prepare polystyrene; 0.2g polystyrene is ultrasonically dispersed in 10mL of 0.2% sodium lauryl sulfate under 80W power In an aqueous solution, a monodisperse...

Example Embodiment

[0043] Example 2

[0044] Taking the preparation of ofloxacin and 17β-estradiol double template molecularly imprinted composite microspheres as an example, the preparation method includes the following steps:

[0045] Step 1: Synthesis of monodisperse polystyrene dispersion

[0046] Put 10.908g styrene, 0.11 azobisisobutyronitrile, 1.09g polyvinylpyrrolidone k-30 with an average molecular weight of 40,000 into a 250mL single-necked flask containing 82g absolute ethanol, styrene and azobisisobutyronitrile, The mass ratio of polyvinylpyrrolidone to anhydrous ethanol is 1:0.01:0.1:7.5, ultrasonically dispersed with an ultrasonic cleaner at a power of 80W for 20 minutes, and nitrogen is blown for 15 minutes to remove oxygen. The other operations are the same as in Example 1. Monodisperse polystyrene dispersion;

[0047] Step 2: Same as Example 1, to prepare monodisperse crosslinked glycidyl methacrylate microspheres.

[0048] Step 3: Same as Example 1, to obtain monodisperse porous crossl...

Example Embodiment

[0055] Example 3

[0056] The preparation method of ofloxacin and 17β-estradiol double-template molecularly imprinted composite microspheres in this embodiment includes the following steps:

[0057] Step 1: Synthesis of monodisperse polystyrene dispersion

[0058] Put 10.908g of styrene, 0.4363g of azobisisobutyronitrile, and 3.2724g of k-30 polyvinylpyrrolidone with an average molecular weight of 40,000 into a 250mL single-necked flask containing 98g of absolute ethanol, styrene and azobisisobutyronitrile , The mass ratio of polyvinylpyrrolidone and absolute ethanol is 1:0.04:0.3:9, and the other operations are the same as in Example 1, to obtain a monodisperse polystyrene dispersion;

[0059] Step 2: Same as Example 1, to prepare monodisperse crosslinked glycidyl methacrylate microspheres.

[0060] Step 3: Same as Example 1, to obtain monodisperse porous crosslinked glycidyl methacrylate microspheres.

[0061] Step 4: Monodisperse crosslinked glycidyl methacrylate microspheres are bon...

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Abstract

The invention relates to a preparation method of ofloxacin and 17beta-estradiol double-template molecularly-imprinted composite microspheres, which comprises the following steps of: preparing monodisperse cross-linked epoxypropyl methacrylate microspheres with vinyl bonded on surfaces by using 1, 4-dioxane as a solvent, and preparing the ofloxacin and 17beta-estradiol double-template molecularly-imprinted composite microspheres by using two antibiotics ofloxacin and 17beta-estradiol as templates and by adopting a surface-imprinting technology. The preparation method disclosed by the inventionhas mild reaction conditions and can be used for preparing the double-template molecularly-imprinted composite microspheres which have better identifying performance for the ofloxacin and the 17beta-estradiol, can be rapidly adsorbed and desorbed and have good stability, so that the simultaneous purification and enrichment of two or more trace components are realized, and references are provided for the simultaneous and rapid detection of multiple medicaments in foods.

Description

technical field [0001] The invention belongs to the technical field of synthesis of molecularly imprinted composite microspheres, in particular to a preparation method of ofloxacin and 17-estradiol dual-template molecularly imprinted composite microspheres. Background technique [0002] Antibiotics have important applications in medicine, but their metabolites are closely related to certain human diseases. They can enter the environment through the metabolism of living organisms, or enter the environment as parent compounds for the synthesis of other new antibiotics along with industrial waste. The health and ecological living environment of human beings and other creatures will cause direct or indirect harm. Sanderson once disclosed in the risk assessment of drugs, according to the degree of harm to human health and the environment, they are ranked in order: antibiotics > estrogen > cardiovascular drugs > antineoplastic drugs. Ofloxacin is the third generation of ...

Claims

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Application Information

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IPC IPC(8): C08J9/36C08J9/26C08J7/16C08J7/12C08L51/00C08F257/02C08F222/14C08F220/32C08F212/08B01J20/26B01J20/285B01J20/30
Inventor 张静马晋琴王玲玲田永乐王艳丽
Owner SHAANXI NORMAL UNIV
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