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Electrochemical sensor based on molecular engram and preparation method and application thereof

A molecular imprinting and electrochemical technology, applied in the field of its preparation and electrochemical sensor, can solve the problems such as interference with dopamine determination, and achieve high sensitivity and good selectivity

Active Publication Date: 2013-07-17
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, AA often interferes with the determination of dopamine in traditional methods

Method used

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  • Electrochemical sensor based on molecular engram and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0033] (1) Synthesis of sulfonated graphene

[0034] 37.5 mg of graphite oxide was dispersed in 37.5 g of water. Then a brown graphene oxide suspension was obtained after ultrasonication for 1 h.

[0035] Pre-reduction: first use 5wt% sodium carbonate solution to adjust the pH of the above-mentioned graphene oxide suspension to 9, mix 300mg NaBH 4 Dissolve in 7.5g water, and then add NaBH 4 The solution was added to the graphene oxide suspension. The mixture was stirred continuously at 80 °C for 1 h. The reduction process was carried out under nitrogen atmosphere. The suspension turned from coffee color to black. Aggregation was observed after the reduction reaction, and after centrifugation and washing with water for 2 to 3 times, the partially reduced graphene oxide was dispersed in 37.5 g of water by ultrasound to obtain a suspension;

[0036] Sulfonation: 23mg p-aminobenzenesulfonic acid and 9mg sodium nitrite in 5g water and 0.25g 1mol L -1React in HCl, form diazon...

Embodiment 2

[0049] (1) Synthesis of sulfonated graphene

[0050] 37.5 mg of graphite oxide was dispersed in 37.5 g of water. Then a brown graphene oxide suspension was obtained after ultrasonication for 1 h.

[0051] Pre-reduction: first adjust the pH of the above-mentioned graphene oxide suspension to 10 with 5wt% sodium carbonate, mix 300mg NaBH 4 Dissolve in 7.5g water, and then add NaBH 4 The solution was added to the above graphene oxide suspension. The mixture was stirred continuously at 80 °C for 1 h. The reduction process was carried out under nitrogen atmosphere. The suspension turned from coffee color to black. Aggregation was observed after the reduction reaction, and after centrifugation and washing with water for 2 to 3 times, the partially reduced graphene oxide was dispersed in 37.5 g of water by ultrasound to obtain a suspension;

[0052] Sulfonation: 23mg p-aminobenzenesulfonic acid and 9mg sodium nitrite in 5g water and 0.25g 1mol L -1 React in HCl to form diazoni...

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Abstract

The invention relates to an electrochemical sensor based on a molecular engram and a preparation method and an application thereof. The electrochemical sensor based on the molecular engram comprises a working electrode, a reference electrode and a counter electrode, wherein the base electrode of the working electrode is a gold electrode; sulfonated graphene is modified on the surface of the gold electrode; after airing, polymerization is performed in an acid buffer solution containing o-phenylenediamine and dopamine till the oxidation peak current of the o-phenylenediamine falls to zero, and then dopamine is eluted in a sulfuric acid medium; and the reference electrode and the counter electrode are a saturated calomel electrode and a platinum wire electrode respectively. Under an optimal condition, a molecular engram sensor with molecule identifying performance is prepared by an appropriate eluting method, so that rapid analysis and detection of the dopamine are realized. The electrochemical sensor based on the molecular engram has the advantages of quick response, high selectivity, high sensitivity and the like on the molecular engram, the aim of identifying molecules is fulfilled, and detection of the dopamine in the presence of high-concentration ascorbic acid is realized.

Description

technical field [0001] The present invention relates to an electrochemical sensor, its preparation method and application, specifically, the present invention relates to an electrochemical sensor based on molecular imprinting, its preparation method and application. Background technique [0002] Dopamine (DA), as an important neurotransmitter, has the function of regulating physical activity, mental activity, endocrine and cardiovascular activity. Low levels of dopamine can cause diseases and disorders such as schizophrenia, Huntington's disease, and Parkinson's disease. Therefore, it is very necessary to establish a method for sensitive, accurate and rapid quantitative detection of dopamine. Normally, dopamine and ascorbic acid (AA) coexist in body fluids such as extracellular fluid of the central nervous system and serum. The oxidation potential of ascorbic acid is very similar to that of dopamine. Therefore, AA often interferes with the determination of dopamine in tra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30
Inventor 吴丹魏琴李贺杜斌张勇马洪敏李燕辛强
Owner UNIV OF JINAN
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