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Combined live vaccine against porcine reproductive and respiratory syndrome, swine fever and pseudorabies, and preparation method thereof

A technology for respiratory syndrome and porcine pseudorabies, applied to medical preparations containing active ingredients, antiviral agents, pharmaceutical formulas, etc., can solve problems such as time-consuming and laborious, cumbersome immunization procedures, and reduced pig immunity

Active Publication Date: 2014-05-14
华威特(江苏)生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many types of single vaccines for pigs on the market, but they all have the disadvantages of cumbersome immunization procedures, high cost, time-consuming and laborious, etc., which directly affect the preventive effect of these three diseases
In addition, since PRRSV can reduce the immunity of pigs (eg, by destroying the normal function of alveolar macrophages) and cause immunosuppression (see, eg, Pang Haiyang et al., 3rd Swine Disease Control Academic Proceedings of the Symposium, pp. 262-265, 2008), so even if porcine reproductive and respiratory syndrome vaccine is co-immunized with other vaccines, it is difficult to achieve the same effect as single-vaccine immunization

Method used

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  • Combined live vaccine against porcine reproductive and respiratory syndrome, swine fever and pseudorabies, and preparation method thereof
  • Combined live vaccine against porcine reproductive and respiratory syndrome, swine fever and pseudorabies, and preparation method thereof
  • Combined live vaccine against porcine reproductive and respiratory syndrome, swine fever and pseudorabies, and preparation method thereof

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Effect test

preparation example Construction

[0065] In some embodiments, the preparation method may include: (1) subculture and culture the susceptible cell line; (2) the highly pathogenic porcine reproductive and respiratory syndrome TJM strain, the attenuated strain of classical swine fever rabbit The C strain and the pseudorabies Bartha K61 strain were inoculated with susceptible cell lines respectively, and were cultured with a maintenance solution to prepare production virus seeds; (3) the prepared production virus seeds were respectively inoculated on mediums full of 90-100% cells, and maintained with The highly pathogenic porcine reproductive and respiratory syndrome TJM strain virus antigen solution, the classical swine fever lathe attenuated strain cytotoxic antigen solution and the pseudorabies Bartha K61 strain virus antigen solution were obtained by liquid culture and proliferation; (4) the highly pathogenic pig breeding Mix it with respiratory syndrome TJM strain antigen solution, swine fever lathe attenuated...

Embodiment 1

[0083] Embodiment 1 uses cell line to produce the method for highly pathogenic porcine reproductive and respiratory syndrome, swine fever and pseudorabies triple live vaccine

[0084] (1) Subculture and cultivation of cells for seedling production:

[0085] The highly pathogenic PRRSV TJM strain vaccine was prepared with Marc-145 cell line. The Marc-145 cell line was digested and passaged by trypsin-EDTA cell dispersion, and passaged one to three. Add cell growth solution and continue culturing at 37°C. When a good monolayer is formed, it is used for continued passage or virus inoculation.

[0086] The BT cell line was used to prepare the C-strain vaccine of the attenuated rabbit strain of CSF. The BT cell line was digested and passaged with trypsin-EDTA cell dispersion, and passaged at a ratio of 1 to 5; cell growth medium was added to continue culture at 37°C, and when a good monolayer was formed, it was used for continued passage or virus inoculation.

[0087] The vaccin...

Embodiment 2 3

[0096] The immunosuppressive test of embodiment 2 triple live vaccines

[0097] The test used 46 healthy piglets aged 21-28 days, and the test was divided into 10 groups. Among the healthy piglets used, the antigens and antibodies of highly pathogenic porcine reproductive and respiratory syndrome, classical swine fever and pseudorabies were all negative. The first group uses 5 test animals, and the single vaccine of the highly pathogenic PRRSV live vaccine TJM strain is injected intramuscularly into the neck, and the dose of the inoculated virus seed is 10 5.0 TCID 50 / ml, 1ml / pig. The second group uses 5 test animals, and the single vaccine of the C strain of classical swine fever rabbitization attenuated cytotoxic vaccine is injected intramuscularly into the neck. 4.0 TCID 50 / ml), 1ml / pig. The third group used 5 experimental animals, and intramuscularly injected a single vaccine of the Bartha K61 strain of the pseudorabies virus vaccine into the neck, and the dose of t...

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Abstract

The invention provides a combined live vaccine for preventing porcine reproductive and respiratory syndrome, swine fever and pseudorabies, and a preparation method and application thereof. According to the invention, no immunosuppression occurs among three vaccine strains of the combined live vaccine; the combined live vaccine is identical with each single vaccine in the aspects of security, immunogenicity, immunity duration and immuno-protective effects, but in the aspect of convenience, the combined live vaccine is more convenient than each single vaccine since prevention of three diseases is realized through only one immunization; thus, work load of immunization and inoculation is mitigated, stress on swinery is reduced, and immunological paralysis and immunological failure caused by frequent immunization are avoided, thereby achieving the effect of preventing porcine reproductive and respiratory syndrome, swine fever and pseudorabies.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and more specifically relates to a triple vaccine for porcine reproductive and respiratory syndrome, swine fever and pseudorabies and a preparation method thereof. Background technique [0002] Porcine reproductive and respiratory syndrome is a highly contagious disease of pigs caused by porcine reproductive and respiratory syndrome virus (Porcine productive and respiratory syndrome virus, PRRSV). A highly contagious viral disease characterized by respiratory symptoms and high mortality in fetuses, stillbirths, weak piglets and piglets. At present, the disease is prevalent in all pig-raising countries in the world, and its prevalence and distribution in China are becoming more and more extensive, and the harm is also becoming more and more serious. [0003] Pseudorabies (PR) is an important infectious disease caused by pseudorabies virus (PRvirus, PRV) in various domestic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/245A61K39/187A61K39/12A61P31/14A61P31/22
Inventor 武华
Owner 华威特(江苏)生物制药有限公司
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