Method for detecting fatty acid content by gas chromatography-mass spectrometry (GC-MS)

A gas chromatography and fatty acid technology, applied in the field of rapid detection of fatty acid content in animal samples, can solve the problems of cumbersome steps, large sample volume, difficult separation, etc., and achieve the effects of simplifying operation steps, reducing sample consumption and improving work efficiency.

Active Publication Date: 2012-10-24
SHAANXI UNIV OF SCI & TECH
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Problems solved by technology

[0007] The determination of free fatty acids in plasma is to first combine fatty acids with Cu 2+ function, after extraction with chloroform, copper reagent was used to determine Cu 2+ concentration, and then get the content of free fatty acid, this method is not only cumbersome, but also can only measure the total amount of fatty acid
Although gas

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  • Method for detecting fatty acid content by gas chromatography-mass spectrometry (GC-MS)
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  • Method for detecting fatty acid content by gas chromatography-mass spectrometry (GC-MS)

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[0027] The present invention will be further explained below with reference to the accompanying drawings, taking the detection of fatty acids in Kunming mouse liver as an example, but the scope of protection of the present invention is not limited to this experiment.

[0028] The present invention provides a method for detecting the content of trace animal fatty acids based on step-by-step one-pot extraction and gas chromatography-mass spectrometry. The specific steps are as follows:

[0029] 1. Sample preparation

[0030] Store the obtained mouse liver tissue in freezing buffer; accurately weigh 10.00mg (±0.02mg) of liver tissue, cut it to about 1mm, and then place the sample in a 5mL screw-top glass bottle. In the testing experiment, the screw-top glass bottle can replace the traditional heating and reflux device. It has the following characteristics: small volume, small sample amount (1-10mg), and good sealing effect (with screw-top bottle cap and pressure resistance 0.2-0.3Mpa)....

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Abstract

The invention provides a method for detecting fatty acid content in animal sample by gas chromatography-mass spectrometry (GC-MS), to solve the problems of complicated sample treatment steps and high cost in conventional methods in practical production and scientific research. The method comprises the steps of treating animal tissue samples; extracting, saponifying and methylating fatty acids; determining GC-MS conditions for fatty acid detection; analyzing data and identifying sample. The method has the advantages of simple operation, low detection limit, good reproducibility, and visual andreliable experiment data; is especially suitable for trace sample detection, can compare fatty acid compositions and contents among different samples, has wider application, and provides a new thought and method for rapid detection of fatty acid composition and content in animal sample for scientific research, clinical application, and food quality and safety monitoring.

Description

technical field [0001] The invention relates to a method for rapidly detecting fatty acid content in animal samples based on gas chromatography-mass spectrometry (GC-MS method). Background technique [0002] Fatty acids are the basic substances that make up human fat and lipids, and are important components of cell membrane phospholipids, which directly regulate the composition of cell membranes, proteins and intramembrane receptors. Fatty acids can be divided into saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFA) according to the degree of saturation. [0003] Short-chain SFA can regulate intestinal motility, promote colon cell proliferation, increase circulating blood flow at intestinal mucosal injury, and promote the recovery of intestinal mucosal structure and function; in addition, short-chain saturated fatty acids can treat diversion colitis , distal ulcerative colitis and radiation colitis. In addition, excessive...

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Application Information

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IPC IPC(8): G01N30/02
Inventor 龚频陈福欣
Owner SHAANXI UNIV OF SCI & TECH
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