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Use of geranylgeranylacetone for preventing and treating renal injuries caused by morphine

A technology for teprenone and kidney damage, applied in the field of medicine, to achieve high safety, reduce economic burden, and significant curative effect

Inactive Publication Date: 2014-05-07
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whether teprenone can be used to prevent and treat kidney injury caused by morphine has not been reported

Method used

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  • Use of geranylgeranylacetone for preventing and treating renal injuries caused by morphine
  • Use of geranylgeranylacetone for preventing and treating renal injuries caused by morphine
  • Use of geranylgeranylacetone for preventing and treating renal injuries caused by morphine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Teprenone inhibits morphine-induced caspase-9 activation in the kidney

[0024] The mice were divided into 4 groups (6 in each group): control group (saline), morphine group (Mor), GGA and morphine group (GGA+Mor), GGA group. From day 1 to day 7, the Saline group and Mor group were given normal saline in advance by gavage, GGA+Mor group and GGA group were given GGA (800 mg / kg) by gavage in advance, and the Saline group and GGA group were given intraperitoneal injection 2 hours later. Saline, Mor group and GGA+Mor group were intraperitoneally injected with morphine (the daily doses were 10, 20, 40, 60, 80, 100 and 100 mg / kg). Two hours after the last morphine injection, the kidney tissues of the mice were separated, the protein was extracted, and the expression of pro-caspase-9 in the kidney cells was detected by Western blotting. figure 1 The results showed that pre-perfusion of GGA can inhibit the decrease of pro-caspase-9 caused by morphine, which indicates t...

Embodiment 2

[0025] Example 2: Teprenone inhibits morphine-induced caspase-3 activation in the kidney

[0026] The mice were divided into 4 groups (6 in each group): control group (saline), morphine group (Mor), GGA and morphine group (GGA+Mor), and GGA group. From day 1 to day 7, the Saline group and Mor group were given normal saline in advance by gavage, GGA+Mor group and GGA group were given GGA (800 mg / kg) by gavage in advance, and the Saline group and GGA group were given intraperitoneal injection 2 hours later. Saline, Mor group and GGA+Mor group were injected intraperitoneally with morphine (the daily doses were 10, 20, 40, 60, 80, 100 and 100 mg / kg). Two hours after the last morphine injection, the kidney tissues of the mice were separated, the protein was extracted, and the expression of caspase-3 in kidney cells was detected by Western blotting. figure 2 The results show that pre-perfusion of GGA can inhibit the increase of active caspase-3 caused by morphine, which indicates tha...

Embodiment 3

[0027] Example 3: Teprenone inhibits the increase in malondialdehyde (MDA) caused by morphine in the kidney

[0028] The mice were divided into 4 groups (6 in each group): control group (saline), morphine group (Mor), GGA and morphine group (GGA+Mor), and GGA group. From day 1 to day 7, the Saline group and Mor group were given normal saline in advance by gavage, GGA+Mor group and GGA group were given GGA (800 mg / kg) by gavage in advance, 2 hours later, the Saline group and GGA group were injected intraperitoneally Physiological saline, Mor group and GGA+Mor group were intraperitoneally injected with morphine (daily injection dose: 10, 20, 40, 60, 80, 100 and 100 mg / kg). Two hours after the last morphine injection, the mouse kidney tissue was separated, prepared into a 10% homogenate with phosphate buffer in an ice bath, and centrifuged in a high-speed refrigerated centrifuge for 10 minutes (4 ℃, 4000 rpm), Take the supernatant and use the malondialdehyde (MDA) ELISA kit to de...

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Abstract

The invention relates to the novel use of geranylgeranylacetone for preventing and treating renal injuries caused by morphine. The geranylgeranylacetone can be used for protecting renal cells from the poison of the morphine, suppressing cell apoptosis caused by the morphine, weakening lipid peroxidation damage caused by the morphine, and preventing and treating the renal injuries caused by the morphine.

Description

technical field [0001] The invention relates to a new application of teprenone, in particular to the application of teprenone in the preparation of prevention and / or treatment of kidney damage caused by morphine, and belongs to the technical field of medicine. Background technique [0002] The abuse of opioids has become a serious social problem at home and abroad. Morphine, a powerful analgesic commonly used to treat acute and chronic pain, is a type of opioid. However, long-term use of morphine can lead to cell death and apoptosis in multiple systems and organs, and its clinical application is greatly limited (Nestler EJ, Aghajanian GK. Molecular and cellular basis of addiction. Science. 1997; 278: 58-63. Yin D, Mufson RA, Wang R, Shi Y. Fas-mediated cell death promoted by opioids. Nature. 1999; 397-218. Hu S, Sheng WS, Lokensgard JR, Peterson PK. Morphine induces apoptosis of human microglia and neurons. Neuropharmacology. 2002;42:829–836.). Among them, the damage of m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/121A61P1/16
Inventor 白洁罗富成邓娟
Owner KUNMING UNIV OF SCI & TECH
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