Separation method for dermatophagoides farinae allergy resistant active substance in propolis

A technology of active substance and separation method, applied in the field of deep utilization of propolis, can solve the problems of cumbersome methods, inability to use indoors for a long time, high toxicity of lindane and not easy to degrade, etc., and achieves the effect of an efficient method system

Inactive Publication Date: 2015-01-21
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] Reducing the number of dust mites can be achieved through physical and chemical methods: common physical methods such as reducing the relative humidity of the room, using dust cloth, replacing carpets, furniture and cleaning bedding in time, etc., but these methods are cumbersome and difficult to control ; As early as 1976, Wharton summed up the indoor activity determination of 30 kinds of chemical reagents on dust mites, only 1% lindane (lindane) had better acaricidal activity
Lindane has not been commercially used as an indoor acaricide due to its high toxicity and not easy to degrade
The commonly used acaricides currently on the market are still mainly focused on agricultural development, such as pyrethroids (Pyrethroids), 1% pirimiphos methyl, etc. Most of them are fumigants and cannot be used indoors for a long time; and most Chemical acaricides can only reduce the number of living organisms, but cannot effectively reduce the number of allergens such as dust mite excreta and residues, and can still cause allergic diseases
Therefore, there is still a big gap in the commercial application of chemical methods to control the number of dust mites.

Method used

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  • Separation method for dermatophagoides farinae allergy resistant active substance in propolis
  • Separation method for dermatophagoides farinae allergy resistant active substance in propolis
  • Separation method for dermatophagoides farinae allergy resistant active substance in propolis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] The establishment of embodiment 1 propolis extraction method system

[0070] 1 Materials and methods

[0071] 1.1 Materials

[0072] 1.1.1 Collection of materials

[0073] Since 2009, relying on Hubei Consinong Bee Products Co., Ltd., the experimenters have obtained propolis samples through direct contact with beekeepers and sampling themselves. The propolis is brown and agglomerated, and the bee species is Italian bee, and the environment where it is collected is mostly poplar and pine.

[0074] The collected samples were refrigerated at -20°C for several days, and when they were in a hard state, they were crushed into small pieces and stored in a 4°C refrigerator for later use.

[0075] Table 2-1 Characteristics of propolis samples from different regions in China

[0076]

[0077]

[0078] 1.1.2 Main instruments

[0079]

[0080] 1.1.3 Main reagents

[0081] Absolute ethanol, methanol, ethyl acetate, and chloroform, all of analytical grade, were purchas...

Embodiment 2

[0112] Example 2 The inhibitory activity of propolis extract to dust mite allergens

[0113] Currently, one of the most important allergens causing allergic diseases in the world is the dust mite (Dermatophagoides farinae). Dust mite, as the main inhalant allergen, can cause allergic diseases in all ages, especially in children. Therefore, how to effectively control dust mites has become a research hotspot of common concern in the medical and pesticide circles.

[0114] 1 Materials and methods

[0115] 1.1 Materials

[0116] 1.1.1 Insects tested

[0117] Dust mite (Dermatophagoides farinae), obtained from the Department of Phraseology and Institute of Tropical Medicine, College of Medicine, Yonsei University since 1999, was bred under laboratory conditions for multiple generations without any acaricide application.

[0118] 1.1.2 Main instruments

[0119] BIO-RAD No.422 electrophoresis tank, BIO-RAD No.041BR electrophoresis instrument, light incubator, high-speed centrifu...

Embodiment 3

[0155] Example 3 Separation and purification of propolis anti-dust mite allergen active substance

[0156] The diversity of biological activity of propolis has been accepted by more and more people, but due to the diversity of its origin and glue source plants, its biological activity is also different, and the material components that exert biological activity are not the same. At present, when researching various biological activities of propolis, it must be organically combined with the research of its active ingredients in order to be better applied to production practice.

[0157] 1 Materials and methods

[0158] 1.1 Materials

[0159] 1.1.1 Test samples

[0160] The 13 kinds of hair propolis samples were taken from the propolis paste obtained by the optimized process in the second chapter, numbered YP-1~YP-13 and placed in a 4°C refrigerator for use. According to the comparison of the inhibitory effect of each sample on the expression of the allergenic protein in the ...

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Abstract

The present invention provides a separation method for dermatophagoides farinae allergy resistant active substance in propolis. The method comprises steps of: first, extracting the propolis with an ethanol solvent; second, conducting solid-liquid separation and rotary evaporation to remove ethanol, so as to obtain an extraction cream of dermatophagoides farinae allergy resistant active substance in propolis. According to the method, the extraction cream is further separated by column chromatography and thin layer chromatography to obtain active components. The method also comprises chemical structure identification on the active components of kaempferol and / or chrysin. The present invention provides a method for separating natural active substances from propolis and novel application of the active substances in resistance of dermatophagoides farinae allergy. The invention not only provides an efficient method and system for deep development and utilization of propolis, but also provides a new way for prophylaxis and treatment of dermatophagoides farinae allergen induced diseases.

Description

technical field [0001] The invention relates to the deep utilization of propolis, more specifically, the invention relates to an extraction method of propolis active substances and the application field of propolis active substances in inhibiting dust mite allergens. Background technique [0002] Propolis English Propolis comes from ancient Greek, which is composed of "pro" (in front) and "lis" (castle), meaning that propolis has the effect of narrowing the entrance of the hive (Castaldo and Capasso 2002). Propolis is generally an opaque colloidal substance formed by worker bees collecting buds and twigs of plants, mixing them with gum, pollen, honey and other substances and sending them to the hive, and being processed and transformed by the tongue glands, wax glands and other glands of office bees. Most of them are brown, taupe, yellowish brown, gray green, dark green, and very few are dark black (Rak1996). Due to the small and humid internal space of the hive, it is cond...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/64A61P33/14A61K35/644
Inventor 王沫陶文琦安龙濬
Owner HUAZHONG AGRI UNIV
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