Application of chrysin in preparation of medicaments for treating autoimmune and inflammatory diseases

An autoimmune, chrysin technology, applied in allergic diseases, bone diseases, sensory diseases, etc., can solve problems such as antigen signal stimulation, and achieve the effects of reducing infiltration, reducing spinal cord demyelination, and inhibiting T cell proliferation.

Inactive Publication Date: 2012-11-28
TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the pathological mechanism of EAE / MS is not fully understood, but it is generally believed that EAE is an inflammatory disease of the central nervous system caused by autoimmune inflammation mediated by helper T cells (TH1 and TH17). Directed differentiation of T cells depends on the stimulation of different antigenic signals derived from dendritic cells

Method used

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  • Application of chrysin in preparation of medicaments for treating autoimmune and inflammatory diseases
  • Application of chrysin in preparation of medicaments for treating autoimmune and inflammatory diseases
  • Application of chrysin in preparation of medicaments for treating autoimmune and inflammatory diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1.1 Isolation of human peripheral blood mononuclear cells

[0028] (1) Go to the blood station to get fresh blood (not more than 8 hours). Approximately 40 mL.

[0029] (2) Dilute with 3 times the volume of PBS (the more diluted blood samples, the better the purification of monocytes), a total of 160 mL. Take 15 mL of lymphocyte separation medium Ficoll-Paque and add it to a 50 mL centrifuge tube. The centrifuge tube is tilted at 45o, absorb 35 mL of diluted blood, and superimpose on the separation liquid along the tube wall at 1 cm above the separation liquid surface. Do not damage the separation interface. . (Divide into 4 tubes, first add 35 mL of blood to each tube of 15 mL of Ficoll-Paque, and then average the remaining blood)

[0030] (3) Centrifuge at 400g at room temperature for 30 minutes, decelerate slowly, and do not set the brake gear.

[0031] (4) Take out the centrifuge tube smoothly, the bottom layer is red blood cells and granulocytes, the middle lay...

Embodiment 2

[0092] Mix 50g of chrysin and 280g of starch evenly, make granules with starch slurry (take 220g of starch and make starch slurry with water), sieve, dry, and pack into capsules.

Embodiment 3

[0094] Mix 80g of chrysin and 340g of starch evenly, use starch slurry (take 210g of starch to make starch slurry with water), make granules, sieve, dry, add 6‰ magnesium stearate, mix well, press into tablets, and coat with film. .

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PUM

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Abstract

The invention discloses application of chrysin in preparation of medicaments for treating autoimmune diseases. Experimental results show that the chrysin can inhibit differentiation of human immune dendritic cells (DC) and maturity of LPS (lipopolysaccharide)-activated human immune DC, can inhibit the antigen uptake capabilities of the immature DC and the antigen presenting capabilities of the mature DC and can inhibit T cell proliferation reactions stimulated by the DC. In particular, the chrysin can reduce the average score, the highest score and the total score of clinical scores of experimental autoimmune encephalomyelitis of mice and can relieve myelitis cell infiltration and demyelination in spinal cords. Thus, the medicament inhibiting immune systems is expected to become the medicament for treating such autoimmune inflammatory diseases as multiple sclerosis, neuromyelitis optica and acute disseminated encephalomyelitis clinically.

Description

technical field [0001] The invention belongs to the technical field of application of traditional Chinese medicines, and relates to the application of Chrysin, a monomer of traditional Chinese medicines, in the preparation of medicines for treating autoimmune diseases and nervous system inflammatory diseases. Background technique [0002] Flavonoids are a class of important natural organic compounds produced in the process of plant metabolism, which are of great significance to the treatment and prevention of human tumors, aging, cardiovascular and other diseases. Especially in the past 30 years, flavonoids have been the focus of research, development and utilization at home and abroad, and they are the source of finding promising lead compounds and biologically active ingredients. Chrysin (Chrysin) namely 5,7. Dihydroxyflavone, a natural flavonoid compound, widely exists in a variety of plants, propolis and honey, and is the main active ingredient of propolis. Studies hav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61P27/02A61P13/12A61K31/7048A61P1/16A61P29/00A61P19/02A61P25/28A61P21/04A61P37/02
Inventor 张荣信葛禛禛张凯薛振毅
Owner TIANJIN MEDICAL UNIV
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