Device and method for biological sample purification and enrichment

A sample and injection port technology, applied in biochemical equipment and methods, biochemical cleaning devices, chemical instruments and methods, etc., to reduce the need for repeated sampling and to quickly diagnose information.

Active Publication Date: 2012-11-28
ICELLATE MEDICAL AB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, despite the available technology, there remains a need in the art for devices and methods that provide efficient and effective purification and enrichment of components in liquid biological samples.

Method used

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  • Device and method for biological sample purification and enrichment
  • Device and method for biological sample purification and enrichment
  • Device and method for biological sample purification and enrichment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1 Flush of bladder contaminated with red blood cells

[0075] Perform bladder flushes on living organisms to collect flushes that may contain suspended cells and contaminants. Preliminary microscopic analysis of stained droplets of the sample confirmed the presence of a large number of red blood cells and a small number of possible target cells, see image 3 . The remaining sample was added to the injection port of the device of the present invention and filtered.

[0076] The processing time is about 10 minutes. The device reaches a maximum pressure of 140mm H when it first draws the sample through the filter 2 O. Material captured in the first aspiration reservoir was placed on slides and stained. Purified and enriched target cells are easily visualized in their native state by microscopy, see Figure 4 .

[0077] For comparison, a second suction reservoir was used. The material captured in the second aspiration reservoir was placed on a slide and stai...

Embodiment 2

[0078] Example 2 Ascites fluid contaminated by epithelial cells

[0079] Ascites fluid samples were obtained. Stained droplets of this fluid, viewed under a microscope, show a large number of epithelial cells and a small number of possible target cells, see Figure 5 . The remaining 10ml of sample was loaded into a 200ml inlet tube and filtered using the device of the present invention.

[0080] The process ends in approximately 10 minutes, during which time a maximum pressure of 140 mm H is reached on the first draw of the sample through the filter 2 O. Material captured in the first aspiration reservoir was placed on slides and stained. Purified and enriched target cells are easily visualized in their native state by microscopy, see Figure 6 .

Embodiment 3

[0081] Example 3 Secondary purification and enrichment

[0082] Samples containing cells, including peripheral blood cells, are provided. The cells are chemically lysed according to known techniques. The cellular content is loaded into the inlet tube of the device according to the invention.

[0083] The first process is performed by using a filter whose pores are able to allow the free passage of most subcellular components but retain the largest: the nucleus. After this operation of the system, the nuclei are retained for subsequent use and the remaining components collected in the second suction reservoir are removed. The system is again ready for use and the components previously collected in the second aspiration reservoir are loaded into the injection port.

[0084] The second process is performed using a filter with pores that allow the free passage of most of the cellular components but retains the large mitochondria from this fraction. After this procedure, the nu...

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PUM

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Abstract

A device for sample purification and enrichment comprising a filtration unit having a filter and an agitator, wherein suction draws a sample across the filter and a back wash flushes materials collected in the filter. The amount of collected material in the filter is determined by a pressure measurer controlling the suction and back wash.

Description

technical field [0001] The invention relates to the field of purification of biological samples. More specifically, the present invention relates to devices and methods capable of isolating minute amounts of target cells from liquid samples containing various other cells and / or contaminants. Background technique [0002] Efficient and effective separation of various components in biological samples is necessary in various fields of research and medicine. Many liquid samples contain many types of cells and / or contaminants of little or no interest, and few cells of interest for further evaluation. Therefore, it would be desirable to be able to isolate minute amounts of target cells from a large number of normally occurring cells, such as blood cells or epithelial cells. [0003] Various techniques already exist in the art for meeting this need. For example, it is known to pass a liquid sample through a filter, thereby purifying certain components of the sample. US Patent 7...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/06B01D37/04C12M1/02C12N5/09B01D35/20
CPCC12N1/02C12M47/02B01D35/20B01D37/04C12M1/02C12M3/06C12M27/00C12N5/0081C12N5/0693
Inventor C·埃里克松J·卡斯特罗L·文马尔
Owner ICELLATE MEDICAL AB
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