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Composite active matters of mask and preparation method of composite active matters

An active substance and mask technology, applied in the field of daily necessities, can solve problems such as facial skin infection, inflammatory response, physical damage to epidermal structure and even gland structure, and achieve the effect of easy operation.

Active Publication Date: 2013-01-30
延荣(海南)企业管理中心(有限合伙)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the facial mask preparation process mainly has the following problems: 1) Infection of the facial skin: daily care operations such as removing "dead skin" and squeezing "acne" of the facial skin of the human body may lead to changes in the structure of the epidermis and even the structure of the glands to varying degrees. physical injury, and lead to varying degrees of inflammatory response
2) Absorption of facial skin: facial skin has a certain absorption function, but it is limited to small molecular substances, and has no absorption effect on large molecular weight protein substances.
The two-way feedback mechanism of the secretion types and the secretion amount of these substances and the concentration control, at present, people have not fully grasped
However, there is currently no research on the preparation of active ingredients in facial masks from cell culture residues.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 facial mask preparation

[0029] 1. Collection of residual liquid of cell culture medium

[0030] During the primary culture of rat mesenchymal stem cells, the non-microbial-contaminated culture solution replaced by fresh complete medium was collected, and the cell fragments were removed by aseptic operation, and placed in a sterilized container at low temperature ( 1-4°C) sealed and stored for later use.

[0031] Difilin Acquired

[0032] The fifth instar of silkworm is the period when the posterior silk gland cells synthesize and secrete a large amount of silk fibroin, the main component of silkworm silk. Dissect the rear silk glands of the 5th instar silkworm, put them into distilled water, and treat them on a shaking table at 4°C for 72 hours, and then sterilize the silk fibroin solution with moist heat. After testing, the silk fibroin obtained by the method does not contain sericin.

[0033] Degradation of trifilin

[0034] Weigh 2g of silk fibroin ...

Embodiment 2

[0043] Embodiment 2 facial mask preparation

[0044] 1. Collection of residual liquid of cell culture medium

[0045] Collect the non-microbial-contaminated culture solution that was replaced when the fresh complete medium was replaced during the primary culture of rat fibroblasts, remove cell debris by aseptic operation, place it in a sterilized container, and store it at low temperature (1- 4°C) sealed and stored for later use.

[0046] Difilin Acquired

[0047] The posterior silk glands of the 5th instar silkworm were dissected, put into distilled water, treated on a shaking table at 4°C for 72 hours, and the silk fibroin solution was sterilized by moist heat.

[0048] Degradation of trifilin

[0049] Weigh 2g of silk fibroin derived from silkworm posterior silk gland that has been sterilized by moist heat, place it in 50mL of the above-mentioned cell culture liquid, and incubate at 37°C for 24 hours to obtain the silk fibroin degradation solution. During or after incuba...

Embodiment 3

[0058] The preparation of embodiment 3 facial mask composite active matter

[0059] Collect rat and rabbit epithelial cells, neuroectodermal cells, hematopoietic cells, stem cells, and gonadal cells in the primary culture process when replacing fresh complete medium without microbial contamination of the culture medium, aseptically remove the cells The fragments are placed in a sterilized container, sealed and stored at low temperature (1-4°C) for future use.

[0060] And prepare facial mask active material according to the method of embodiment 1 or 2 respectively.

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Abstract

The invention relates to a mask process of daily necessities, in particular to an application of residual primary culture fluids of mammalian cells in the preparation of active matters of a mask. According to the invention, the residual cell culture fluids are sources of growth factor type matters at home and abroad, and are inexpensive and high in quality, renewable, safe and reliable; silk fibroins can be effectively degraded at the normal temperature by enzymes contained in the residual cell culture fluids, so that small molecular silk fibroins are obtained, and the operation is simple and safe; then sericin in the silk fibroins has immunogenicity so that anaphylaxis may be caused, and the posterior silk gland-derived silk fibroins are completely free of sericin so that the possibility of anaphylaxis is greatly reduced; and the film-forming property of a silk mask is the difficulty of the preparation, a silk I conformation of protein molecules is gradually transformed to silk II in the process of repeatedly stirring and drying the silk fibroins at a high temperature, so that the silk fibroins are stable in structure and have excellent mechanical properties.

Description

technical field [0001] The invention belongs to the field of daily necessities, in particular to a preparation process of a facial mask. Background technique [0002] Mask is one of the most commonly used cosmetics in people's daily life. It is the most guaranteed function of current facial mask products to replenish moisture to human facial skin. Yet the problem that people expect to solve more is that facial mask can promote the metabolism of human face skin, plays cosmetic effect. For this reason, need to develop a kind of facial mask with skin nutrition, wherein main active substance is the key whether facial mask can reach expected effect. [0003] At present, the facial mask preparation process mainly has the following problems: 1) Infection of the facial skin: daily care operations such as removing "dead skin" and squeezing "acne" of the facial skin of the human body may lead to changes in the structure of the epidermis and even the structure of the glands to varyin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61K8/64A61Q19/00
Inventor 周婵王永亮曾秀马群忠
Owner 延荣(海南)企业管理中心(有限合伙)
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