Application of yew in preparation of drug for eczema treatment, and composition
A technology of yew and composition, applied in the field of yew seaweed mud composition
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Embodiment 1
[0015] Embodiment 1: the preparation of yew seaweed mud
[0016] Preparation of sodium alginate gel: Polymerization was carried out using a three-way reaction system of sodium alginate-potato starch-acrylic acid. Among them, sodium alginate / water (w / w)=1 / 10 in sodium alginate solution, starch / water (w / w)=20 / 1 in starch solution, and sodium alginate / starch (w / w) in ternary reaction system w)=1 / 4, the neutralization rate of acrylic acid is 75%, and the dosage is 4 times (w / w) of sodium alginate. After starch gelatinization at 80°C for 0.5 hours, mix with sodium alginate solution and acrylic acid, and add initiator, the amount of initiator (ammonium persulfate) is 1.5% (w / w) of sodium alginate, crosslinking agent is not added, After reacting at 55°C for 2.5 hours, it was dried at 110°C to obtain a sodium alginate gel.
[0017] Add 10 times the mass of Chinese yew to 60% (v / v) ethanol and impregnate for 24 hours, then carry out percolation extraction at a flow rate of 1 mL / min. ...
Embodiment 2
[0019] Embodiment 2: the in vitro antibacterial test of yew seaweed mud
[0020] Objective: To observe the antibacterial effect of yew seaweed mud external preparation on Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli in vitro.
[0021] Materials and Methods:
[0022] 1.1 Experimental materials
[0023] 1.1.1 Drugs
[0024] The yew seaweed mud prepared according to the method of Example 1 is provided by Zhejiang Academy of Traditional Chinese Medicine, batch number: 20110726, 1g of yew seaweed mud contains 0.0476g of yew extract powder.
[0025] 1.1.2 Strains
[0026] The tested strains (Staphylococcus aureus ATCC25922, Staphylococcus epidermidis ATCC12228, Escherichia coli ATCC25923) were provided by the Microbiology Laboratory of Zhejiang Provincial Tongde Hospital.
[0027] 1.1.3 Main instruments and reagents
[0028] Biological safety cabinet, 4°C refrigerator, 37°C constant temperature incubator, high-pressure steam sterilizer, electronic bala...
Embodiment 3
[0045] Example 3: Research on the effect of yew seaweed mud external preparation on eczema
[0046] 1. Test material:
[0047] Prednisone tablets: Zhejiang Xianju Pharmaceutical Co., Ltd., batch number (110310);
[0048] Medication for the high-dose group of yew seaweed mud: mix the yew extract prepared by the method in Example 1 with sodium alginate gel at a mass ratio of 1:10;
[0049]Taxus seaweed mud low-dose group medication: mix the yew extract prepared by the method in Example 1 with sodium alginate gel at a mass ratio of 1:20;
[0050] 2. Experimental method
[0051] 48 male ICR mice were divided into 5 groups on average, 12 in each group, marked as model group, positive group, low-dose yew seaweed mud group, high-dose yew seaweed mud group and control group (yew alone) .
[0052] The positive group was given prednisone (according to the weight of each mouse at 10mg / g) every day, the model group was given an equal volume of distilled water, and the yew seaweed mud ...
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