Extraction method of aucubin and application of aucubin

A technology of aucubin and psyllium extract, which can be applied to chemical instruments and methods, medical preparations containing active ingredients, and pharmaceutical formulas, etc., can solve the problem of cognitive response and processing ability decline, information recognition and processing. , integration and other process obstacles, learning and memory impairment and other problems

Inactive Publication Date: 2013-02-06
薛宏宇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have confirmed that the reduction of cerebral blood flow can hinder the brain's understanding, processing, and integration of inform

Method used

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  • Extraction method of aucubin and application of aucubin
  • Extraction method of aucubin and application of aucubin
  • Extraction method of aucubin and application of aucubin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Separation and purification of aucubin

[0017] ①Psyllium 5.0kg, pulverized by a pulverizer, soaked overnight with 10 times the volume fraction of 70% ethanol, then ultrasonically extracted 3 times, 30min each time, combined the extracts, recovered ethanol in a low-temperature vacuum, concentrated on a water bath to 1000mL, after centrifugation, take about 800mL of the supernatant, inject it into the pretreated AB28 macroporous adsorption resin, wash away sugar and other impurities with distilled water, elute with 10% ethanol solution, 250mL is a fraction collected and eluted Liquid, thin-layer chromatography detection, Ehrlich reagent is chromogen, chloroform-methanol is developer, collects and merges the cut that contains target point;

[0018] ②The above-mentioned fractions were concentrated under reduced pressure, and the concentrate was subjected to silica gel column chromatography, using chloroform-methanol as the eluent, detected by thin-layer chromato...

Embodiment 2

[0019] Example 2: Structural Identification of Monomers

[0020] White amorphous powder, soluble in methanol and ethanol. The molecular weight is 346.33, and the molecular formula is C 15 h 22 o 9 . The NMR spectrum data are as follows:

[0021] 1 H-NMR (CD 3 OD) δppm: 5.16 ( 1 H,dd,J=6.25Hz,C 1 -H),6.25( 1 H,dd,J=6.1Hz,C 3 -H),4.69( 1 H,d,J=6.9Hz,C 4 -H),2.54( 1 H,m,C 5 -H),4.88( 1 H,d,J=7.8Hz,C 6 -H),5.48( 1 H,br,C 7 -H),2.94( 1 H,br,C 9 -H),4.34( 1 H,d,J=15.3Hz,C 10 -H),4.61( 1 H,d,J=14.9Hz,C 10 -H),4.64( 1 H,d,J=7.1Hz,C 1 '-H),3.65( 1 H,dd,J=12.1Hz,C 6 '-H), 3.87( 1 H,dd,J=11.9Hz,C 6 '-H). 13 C-NMR (CD 3 OD) δppm: 97.72 (C-1), 141.69 (C-3), 106.32 (C-4), 45.38 (C-5), 82.83 (C-6), 130.76 (C-7), 149.0 (C- 8),48.14(C-9),61.42(C-10),98.81(C-1'),75.02(C-2'),77.90(C-3'),71.57(C-4'),76.32 (C-5'), 63.01 (C-6').

[0022] As mentioned above, the compound is β-D-glucopyranoside, ie aucubin.

Embodiment 3

[0023] Example 3: Effects of Single Administration of Monomer on Blood Glucose in Normal SD Rats

[0024] 56 normal rats were taken and randomly divided into 7 groups, 8 in each group. Normal saline, insulin, and different doses of aucubin (administrated by intraperitoneal injection) were injected intraperitoneally. After 15 days of continuous administration, they were fasted for 4 hours, and blood was collected from the tail to detect the blood sugar level. The results are shown in Table 2. Aucubin at different doses has no effect on the blood sugar level of rats.

[0025] Table 1 Effect of one administration on blood sugar content in normal rats (n=8)

[0026]

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PUM

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Abstract

The invention relates to an extraction method of aucubin and an application of the aucubin. The method comprises the simple steps of crushing plantain, soaking in alcohol overnight, conducting ultrasonic extraction, merging, recovering, concentrating and centrifuging an extracting solution, obtaining supernate, adopting macroporous resin and eluting an alcohol solution, conducting thin-layer chromatography detection, collecting and concentrating fraction at a target point, conducting silicagel column chromatography on a concentrate, and then collecting and concentrating the fraction at the target point, repeating the silicagel column chromatography to obtain macrocrystalline, purifying a crude product through high performance liquid chromatography, collecting target components, freezing and drying, and finally obtaining white amorphous powder. The substance extracted from the plantain by the method is the aucubin. The aucubin can be used for treating and preventing diabetes mellitus and concurrent encephalopathy of the diabetes mellitus, the occurrence and development mechanisms of the diabetes mellitus and complications of the diabetes mellitus are further deeply studied, and new-generation drugs for resisting the diabetes mellitus and the complications of the diabetes mellitus can be developed.

Description

technical field [0001] The present invention relates to a method for extracting aucubin and the application of aucubin. Background technique [0002] Diabetes mellitus (DM) is a chronic metabolic disease, clinically manifested as "three more and one less" symptoms of polydipsia, polyphagia, polyuria and weight loss. It is a direct or indirect insulin secretion or insufficient action, and chronic hyperglycemia and metabolic disorders of carbohydrates, lipids and proteins. The direct consequence of diabetes metabolic disorder is to produce various complications. Diabetes is extremely harmful to people's health and can damage various systems of the body, such as: cardiovascular and cerebrovascular, kidney, eyes, nervous system, etc. Diabetic Neuropathy (DN) is a common complication in diabetic patients. Some data show that the incidence of DN is as high as 60% to 90%, and it can even affect all diabetic patients. Insufficient insulin secretion leads to hyperglycemia. Long-t...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08A61K31/7048A61P3/10A61P25/28
Inventor 不公告发明人
Owner 薛宏宇
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