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Liquid chromatogram tandem mass spectrum measuring method for creatinine in urine

A technology of tandem mass spectrometry and liquid chromatography, which is applied in the field of determination of creatinine in urine by liquid chromatography tandem mass spectrometry, achieving the effects of simple pretreatment, easy operation, good sensitivity and repeatability

Inactive Publication Date: 2013-03-20
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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Problems solved by technology

However, creatinine has a retention time of 0.59 min on a reversed-phase C18 column, making it difficult to separate from water-soluble interferences in urine

Method used

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  • Liquid chromatogram tandem mass spectrum measuring method for creatinine in urine
  • Liquid chromatogram tandem mass spectrum measuring method for creatinine in urine
  • Liquid chromatogram tandem mass spectrum measuring method for creatinine in urine

Examples

Experimental program
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Effect test

example 1

[0048] 3. Determination method: draw 1, 2, 5, 10, 20, 50, 200, 500 and 2000 ng / mL standard solutions and 5 μL of diluted and filtered samples, inject LC-MS / MS system for separation and analysis, and measure The amount of creatinine in the sample.

[0049] Example 2: As described in Example 1, 37 urine samples were taken. The creatinine content in urine was measured as shown in Table 3.

[0050] serial number content serial number content 1 13333.33 15 10087.72 2 12587.72 16 4008.77 3 7706.14 17 11008.77 4 9605.26 18 16008.77 5 5337.72 19 5342.11 6 4114.04 20 3114.04 7 4236.84 21 2605.26 8 13070.18 22 14429.82 9 5438.60 23 7605.26 10 2578.95 24 2048.25 11 12587.72 25 11052.63 12 8289.47 26 4013.16 13 10789.47 27 8087.72 14 13859.65 28 15000.00

example 2

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Abstract

The invention discloses a liquid chromatogram tandem mass spectrum measuring method for creatinine in urine. The method is characterized by comprising the steps that a sample is diluted, and the measurement is conducted by introducing a liquid chromatogram- electrospray ionization-tandem mass spectrometer, so that the content level of the creatinine in the urine can be detected rapidly and accurately. According to the measuring method, a perylene standard product serves as an internal standard quantitative analysis substance, so that errors caused during sample preprocessing are reduced; and the selectivity and the accuracy of the method are improved better through the tandem mass spectrum method. The chromatographic process is improved better and the chromatographic analysis time is reduced through a chromatographic column and the selection and optimization of the elution condition.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of physical and chemical testing of urine samples, and in particular relates to a method for measuring creatinine in urine by liquid chromatography tandem mass spectrometry. Background technique: [0003] Continuous and complete collection of 24-hour urine from volunteers during the experiment can provide more accurate toxicant exposure dose levels. The integrity of the urine sample is the first concern of the experiment organizer. Generally, the 24-hour clearance rate of creatinine is used to determine the integrity of the sample, or the creatinine ratio is used to correct the urine concentration. In recent years, creatinine has been widely used as a correction factor in the concentration correction of smoke-related biomarkers, including cotinine, NNAL, and mercaptouric acid. [0004] The detection methods of creatinine in urine mainly include Jaffe method, enzymatic spectrophotometry, HPLC me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 侯宏卫张小涛田永峰刘彤陈欢韩书磊胡清源
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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