Bacillus pumilus and application thereof in prevention and control of comma bacillus in aquatic product

A Bacillus pumilus, Vibrio cholerae technology for bacteria, biological water/sewage treatment, microorganism-based methods, etc.

Inactive Publication Date: 2013-03-27
苏州市水生动物疫病预防控制中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a Bacillus pumilus AB3 (Bacillus pumilus AB3), which can effectively eliminate Vibrio cholerae in the aquaculture system with the effect of antagonistic bacteria, and solve the problem of Vibrio cholerae carried by aquatic products in the prior art. Epidemic prevention

Method used

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  • Bacillus pumilus and application thereof in prevention and control of comma bacillus in aquatic product
  • Bacillus pumilus and application thereof in prevention and control of comma bacillus in aquatic product
  • Bacillus pumilus and application thereof in prevention and control of comma bacillus in aquatic product

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Vibrio cholerae antagonistic bacteria search, screening, identification

[0021] (1) Search and screening of Vibrio cholerae antagonistic bacteria

[0022] A) raw materials

[0023] Vibrio cholerae: It was isolated from the water body of soft-shelled turtles in a greenhouse. It was identified as Vibrio cholerae, and its serotype was O139.

[0024] Fishery microecological preparations are selected from six categories of photosynthetic bacteria, bacillus, lactic acid bacteria, nitrifying bacteria, yeast fungi, and compound fungi, with a total of 28 varieties, purchased from fishery drugstores.

[0025] Medium reagents include: tryptone soybean agar medium, nutrient broth medium, purchased from reagent companies.

[0026] Preparation of tryptone soybean agar medium (TSA): Weigh 42g, dissolve it in a conical flask of 1000ml distilled water, stir and dissolve, sterilize with high pressure steam at 121°C for 15min, then pour it into a petri dish, store it at 4°C f...

Embodiment 2

[0041] Trial production of embodiment 2 antagonistic bacteria preparation (fermentation agent)

[0042] According to the biological characteristics of the best Vibrio cholerae antagonistic bacteria Bacillus pumilus AB3 obtained by screening, a suitable medium is screened out, and appropriate fermentation conditions are adopted to make the antagonistic bacteria into a fermentation broth, and an appropriate amount of stabilizer is added. Prepare a certain concentration of Vibrio cholerae antagonistic bacteria starter.

[0043] Concretely adopted fermentation conditions are as follows:

[0044] Fermentation strain: Vibrio cholerae antagonistic bacteria Bacillus pumilus AB3.

[0045] Fermentation medium formula: peptone 10-80 grams, sucrose 20-100 grams, yeast powder 1-10 grams, purified water 1000 ml.

[0046] Fermentation temperature: 28-30°C.

[0047] Fermentation period: 64 hours.

[0048] Rotational speed of fermentation shaker: 150-200r / min.

[0049] Stabilizer: Tween-205...

Embodiment 3

[0051] Embodiment 3 application simulation test

[0052] According to the investigation results of the actual conditions of soft-shelled turtle breeding in greenhouses (including the same stocking mode, feeding management, water temperature, pH, etc.), 5 water tanks were used in the laboratory (specifications: length × width × height = 0.5 m × 0.4 m × 0.3 m) to establish a group of test models similar to the actual soft-shelled turtle breeding conditions, add 40 liters of tap water to each water tank, and after 24 hours of aeration, put 8 soft-shelled turtle fry (average 60 g / head) into each of the five water tanks, The amount of soft-shelled turtle feed is 1% of its body weight every day, and it is fed twice in the morning and evening. Another water tank is not put into soft-shelled turtle and feed, only adds 40 liters of tap water, as blank control. During the entire modeling period, the water body was kept oxygenated, the water temperature was maintained at 27-28°C, and th...

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Abstract

The invention discloses bacillus pumilus AB3. The bacterial strain has a preservation name called bacillus pumilus AB3, of which the preservation number is CGMCC NO.6408, and has the capability of inhibiting the growth or the reproduction of comma bacillus. The bacteria which serve as the comma bacillus antagonistic bacteria can be prepared to antibacterial agents, and the antibacterial agents can be applied to aquaculture, can be used as beneficial microorganism preparations replacing chemical sanitizers, are used for improving the water quality of fishery industry and removing the comma bacillus in an aquatic product cultivation system so as to prevent the damage of the comma bacillus to aquaculture objects and promote the development of the aquaculture industry, and can avoid carrying the comma bacillus in the aquatic products to guarantee the human health safety.

Description

technical field [0001] The invention relates to a strain of Bacillus pumilus, its preparation and its application in the prevention and control of Vibrio cholerae in aquatic products, belongs to the field of fishery ecological safety and aquatic product hygiene and safety, and is used for improving fishery water quality and suppressing and killing cholera arcus in aquaculture systems bacteria. Background technique [0002] Vibrio cholerae (VC) is the pathogen that causes cholera, a severe infectious disease in humans. It is listed as a class A infectious disease in my country and is listed as a quarantine infectious disease internationally. The natural habitat of Vibrio cholerae is the aquatic ecosystem. It is native to the water environment and is a symbiotic body of aquatic animals and plankton. It uses the chitin of aquatic animals as the nutrient matrix. It has the characteristics of mesophilic, alkaliphilic and nutrient salt. It can produce toxins and cause cholera; it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07
Inventor 陈文怡张元柱
Owner 苏州市水生动物疫病预防控制中心
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