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A new method for purification of Lycium barbarum polysaccharide

A technology of Lycium barbarum polysaccharides and a new method, which is applied in the field of purification of Lycium barbarum polysaccharides and Lycium barbarum polysaccharides by macroporous adsorption resin-silica gel chromatography coupling method, can solve the problems of high process cost, separation, and poor application effect, and achieve anti-pollution Strong ability, obvious separation effect and fast exchange speed

Inactive Publication Date: 2016-04-13
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction process of Lycium barbarum polysaccharides in the above-mentioned patent documents is mainly refined through the steps of raw material pretreatment, ultrasonic-assisted extraction, and alcohol precipitation separation. Far inferior to the separation effect of macroporous adsorption resin coupled with silica gel chromatography

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Weigh 10 kg of Lycium barbarum fruit, crush it into a coarse powder, add 18 times the amount of 90% methanol to reflux and extract for 140 minutes, and filter; the filtrate is recovered from methanol, concentrated to 4 Baume, and concentrated with hydrochloric acid:water solution with a volume ratio of 1:1. The pH value of the liquid was adjusted to 2.8, left for 40 minutes, and filtered; the filtrate was placed on a HPD100 macroporous resin adsorption column, and the impurities were washed with water after adsorption, and then washed with 15% methanol, and then eluted with 70% methanol, polysaccharides The semi-finished product eluent, the polysaccharide semi-finished product eluent is recovered from methanol, concentrated and dried polysaccharide semi-finished product; the resulting polysaccharide semi-finished product is put on a silica gel chromatography column, and the mixture of acetone and methanol with acetone:methanol volume ratio of 2:1 washes impurities, and th...

Embodiment 2

[0022] Weigh 15 kg of Lycium barbarum fruit, crush it into coarse powder, add 20 times the amount of 95% methanol to reflux and extract for 160 minutes, and filter; The pH value of the solution was adjusted to 3, left for 40 minutes, and filtered; the filtrate was placed on a HPD400 macroporous resin adsorption column, and the impurities were washed with water after adsorption, and then washed with 10% methanol, and then eluted with 60% methanol, polysaccharides Semi-finished product eluent, polysaccharide semi-finished product eluent recovers methanol, concentrates and dries the polysaccharide semi-finished product; puts the obtained polysaccharide semi-finished product on a silica gel chromatography column, and washes impurities with a mixture of acetone and methanol with a volume ratio of acetone:methanol of 1:1, and then washes the impurities with Acetone:methanol volume ratio of 7:1 acetone and methanol mixture elution, polysaccharide eluent 3.5L; add 140g injection of act...

Embodiment 3

[0024] Weigh 20 kg of Lycium barbarum fruit, crush it into a coarse powder, add 15 times the amount of 87% methanol to reflux and extract for 120 minutes, and filter; The pH value of the solution was adjusted to 2.5, left for 40 minutes, and filtered; the filtrate was placed on an AB-8 macroporous resin adsorption column, and the impurities were washed with water after adsorption, and then washed with 5% methanol, and then eluted with 80% methanol. The polysaccharide semi-finished product eluent, the polysaccharide semi-finished product eluent is recovered from methanol, concentrated and dried polysaccharide semi-finished product; the gained polysaccharide semi-finished product is put on a silica gel chromatography column, and the mixture of acetone and methanol with acetone:methanol volume ratio of 3:1 washes impurities, Then use acetone:methanol volume ratio of 5:1 mixture of acetone and methanol to elute, polysaccharide eluent 5L; add 200g injection of activated carbon, stir...

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PUM

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Abstract

The invention discloses a novel purification method of lycium chinense polyhexose, which comprises the steps that lycium chinense fruits are smashed into crude powder, 15 times of 85% methanol is added for reflux extraction for 120min and filtered; methanol is recovered from a filtrate; the filtrate is concentrated to 3 Be; hydrochloric acid and water at a volume ratio of 3:1 are used to regulate a pH value of a concentrated solution to 2.5; the concentrated solution is placed for 20 min and filtered; a broad-spectrum macroporous resin adsorption column is placed in the filtrate for collection of an eluent; methanol is recovered; the eluent is concentrated and dried to form a polysaccharide semifinished product; a silica gel chromatographic column is placed in the polysaccharide semifinished product; acetone and carbinol at a volume ratio of 3:1 are used to wash impurities; acetone and methanol at a volume ratio of 7:1 are used for elution; an eluent is collected; the collected polysaccharide eluent is refined to form the lycium chinense polysaccharide finished product; and the content is over 70%. The novel purification method has the greatest characteristics that with the adoption of a macroporous adsorption resin-silica gel chromatography coupled method, the novel purification method is quick and convenient; sources of raw materials are abundant; the manufacturing cost is low; the separating effect is obvious; and the extraction purity is high.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for purifying wolfberry polysaccharide, in particular to a new method for purifying wolfberry polysaccharide by using a macroporous adsorption resin-silica gel chromatography coupling method. Background technique: [0002] Lycium chinense, scientific name: Lyciumchinense, alias Gouqizi, wolfberry red fruit, beet seed, wolfberry, dog milk, red green pepper, wolfberry, wolfberry fruit, ground bone, wolfberry eggplant, red earrings, blood wolfberry, wolfberry sprouts , Lycium barbarum, Lycium barbarum, Blood barbarum, Jin wolfberry are the fruits of Lycium barbarum, a plant of the genus Lycium barbarum in the family Solanaceae. Contains betaine (betane), atropine (aTCMLIBopine), gyoscyamine (gyoscyamine), wolfberry polysaccharides, and also contains zeaxanthin, physalis physene, cryptoxanthin (cryptosxanthin), scopolamine (scopo-letin), carrot Vitamin C, thiamine, r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 姚德坤姚德利张亚红姚航黄运生吴海龙
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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