Chrysoperla sinica larvae artificial feed and determination method for toxic effects of insecticidal compounds on Chrysoperla sinica larvae

A technology of Chinese lacewings and artificial feed, applied in animal feed, animal feed, measuring devices, etc., to achieve high-sensitivity effects

Inactive Publication Date: 2014-04-16
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Application Information

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Problems solved by technology

However, as of now, there is no test method suitable for evaluating the direct toxicity of insecticidal compounds to lacewing larvae

Method used

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  • Chrysoperla sinica larvae artificial feed and determination method for toxic effects of insecticidal compounds on Chrysoperla sinica larvae
  • Chrysoperla sinica larvae artificial feed and determination method for toxic effects of insecticidal compounds on Chrysoperla sinica larvae
  • Chrysoperla sinica larvae artificial feed and determination method for toxic effects of insecticidal compounds on Chrysoperla sinica larvae

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Embodiment Construction

[0035] 1 Introduction

[0036] When evaluating the impact of insecticides and transgenic insect-resistant crops on non-target organisms, the primary work is to study the effects of exogenous insecticidal proteins expressed by insecticides or transgenic insect-resistant crops (hereinafter collectively referred to as insecticidal compounds) on selected non-target organisms. direct toxicity. Conducting such experimental work requires the development of a bioassay system in which insecticidal compounds can be fed directly to test organisms. The invention provides a high-efficiency artificial feed for lacewing larvae. Based on the artificial feed, a test model for evaluating the potential toxic effects of insecticidal compounds on lacewing larvae is established. The lacewing larvae artificial feed and toxicity determination model provided by the invention are described in detail below.

[0037] 2. Materials and methods

[0038] 2.1 Source of tested insects

[0039] Based on pre...

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Abstract

The invention relates to a Chrysoperla sinica larvae artificial feed and a determination method for toxic effects of insecticidal compounds on Chrysoperla sinica larvae. The method adopts an artificial feed mixed with an insecticidal compound to be detected to feed Chrysoperla sinica larvae, and the potential toxicity of insecticidal compound on the Chrysoperla sinica larvae is evaluated through the growth and development index of the Chrysoperla sinica larvae. The growth and development index of the Chrysoperla sinica larvae is one or more selected from survival rate, duration and weight. The method adopts an inorganic compound feed, namely potassium dihydrogen arsenate (36 micrograms / g) and / or an organic compound feed, namely snowflakes agglutinin (1wt%) as a positive control compound, and the pure feed is treated as a negative control. By using the Chrysoperla sinica larvae artificial feed provided by the invention, the survival rate, the weight and the growth duration of the Chrysoperla sinica all show a significant dose-effect relationship; and the determination method provided by the invention has high sensitivity, thus being suitable for evaluation of the potential toxicity of exogenous insecticidal proteins on Chrysoperla sinica.

Description

technical field [0001] The invention relates to a method for determining the toxicity of artificial feed for lacewing larvae and insecticidal compounds on lacewing larvae. Background technique [0002] Lacewings such as the common lacewing (Chrysoperla carnea Stephens) and the Chinese lacewing (Chrysoperla sinica Tjeder) have a very wide geographical distribution and are important predatory insect natural enemies on a variety of food and economic crops. In addition, lacewings are often used as indicator organisms for environmental risk assessment of insecticides and transgenic insect-resistant crops because they are easy to raise on a large scale under laboratory conditions and are inexpensive to operate. [0003] When evaluating the impact of insecticides and transgenic insect-resistant crops on non-target organisms, the primary work is to study the effects of exogenous insecticidal proteins expressed by insecticides or transgenic insect-resistant crops (hereinafter collect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/18A23K1/10A23K1/16A23K1/175G01N33/00
Inventor 李云河胡龙彭于发
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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