Determination method for the toxic effects of gastric toxic insecticides or transgenic insecticidal proteins on the mole beetle

A technology of Coccinellidae and insecticidal protein, which is applied in the field of determination of the toxicity of stomach-toxic insecticides or transgenic insecticidal proteins on Coccinella chrysanthemum, and achieves the effect of simple and feasible method.

Active Publication Date: 2015-08-19
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, there is currently no relatively complete technical system that can be used to evaluate the direct toxicity of insecticides or transgenic insecticidal proteins on M.

Method used

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  • Determination method for the toxic effects of gastric toxic insecticides or transgenic insecticidal proteins on the mole beetle
  • Determination method for the toxic effects of gastric toxic insecticides or transgenic insecticidal proteins on the mole beetle
  • Determination method for the toxic effects of gastric toxic insecticides or transgenic insecticidal proteins on the mole beetle

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Embodiment Construction

[0018] The invention provides a method for simply and sensitively detecting the direct toxic effects of stomach poisoning insecticides or transgenic insecticidal proteins on the mosaic ladybird. In this method, insecticides or transgenic insecticidal proteins are uniformly mixed into the established pollen-based diet and directly fed to the ladybug, and the diet mixed with protease inhibitor E-64 (225 μg E-64 / g pollen) was used as positive control, and the feed without any insecticidal compound was used as negative control. The effectiveness of insecticidal compounds was evaluated by comparing the survival rate, developmental duration, body weight and egg production of ladybugs in the treatment group and the control group. Potentially toxic. This method was used to measure the biological response of the chelonian ladybug to different concentrations of E-64, and the different life parameters of the chelonian ladybug showed a significant dose-effect relationship with the change...

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Abstract

The invention provides a method for detecting toxicity influence of stomach toxicity pesticides or transgene insecticidal protein to Propylaea japonica. The method is characterized in that the pesticides or transgene insecticidal protein can be uniformly mixed in a feed which takes pollen as a base to directly feed in Propylaea japonica, taking a feed (225mumg E-64 / g of pollen) mixed with a protease inhibitor E-64 as a positive control, taking the feed without mix of any insecticidal compound as a negative control, and evaluating the latent toxicity of the insecticidal compound by comparing the important life index of Propylaea japonica such as survival rate, growth stage, body weight and oviposition amount of a processing group and a control group. According to the invention, the method is employed to detect the biology reaction of Propylaea japonica to E-64 with different concentration, different life parameters of Propylaea japonica display obvious a dosage-effect relation with the changer of E-64 concentration, so that validity and sensitivity of the method can be proved.

Description

technical field [0001] The invention relates to a method for determining the toxic effects of gastric poisonous insecticides or transgenic insecticidal proteins on the mosaic lady beetle. Background technique [0002] Predatory ladybugs (Coleoptera: Coccinellidae) are common and important natural enemy insects in the field ecosystems of various crops such as cotton, corn, rice, vegetables, etc., and play an important role in crop pest control. Therefore, in the study of the environmental safety impact of pesticides and transgenic plants, this kind of natural enemy insects has always been concerned. Especially in recent years, the widespread planting of transgenic insect-resistant crops, which may have negative effects on natural enemy insects, has aroused widespread concern from relevant scientists. The potential impact of transgenic insect-resistant crops such as Bt crops on ladybug natural enemies has also become an important content of environmental safety assessment of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/00A23K1/18A23K1/14A23K1/175
Inventor 李云河张小洁彭于发
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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