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Method for determining potential toxicity influence of insecticidal compounds on Laodelphax striatellus

A measurement method, the technology of SBPH, is applied in the field of determination of the potential toxicity of insecticidal compounds to SBPH, and can solve the problem of no detection of planthopper insecticidal genes

Inactive Publication Date: 2014-06-04
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, the currently bred transgenic insect-resistant rice has good resistance to Lepidoptera pests, and no insecticidal gene with high resistance to planthoppers has been found so far.

Method used

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  • Method for determining potential toxicity influence of insecticidal compounds on Laodelphax striatellus
  • Method for determining potential toxicity influence of insecticidal compounds on Laodelphax striatellus
  • Method for determining potential toxicity influence of insecticidal compounds on Laodelphax striatellus

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Embodiment 2

[0036]The specific configuration methods of the artificial feed shown in Table 1-Table 4 are as follows: Amino acids, vitamins (except vitamin C) and trace metals (except ferric chloride hexahydrate) are respectively prepared as 2×, 10× and 100× Mother liquor, stored at -20°C. Add the amino acid mother liquor to the large beaker, then add the stock solutions of sucrose, potassium dihydrogen phosphate, magnesium chloride hexahydrate, and ferric chloride hexahydrate, and stir until completely dissolved. Then add vitamin and trace metal mother liquor thereto, and vitamin C solution (prepared separately as 10× mother liquor before use). Adjust the pH of the feed solution to 6.8 with 4% KOH solution, and then dilute to the desired concentration with distilled water. Finally, the solution was filtered and sterilized with a disposable Millipore microporous (0.45 μm) filter, and stored at -20°C for future use.

[0037] 3 Survival fitness of SBPH feeding on the artificial diet...

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Abstract

The invention provides a method for determining influence of insecticidal compounds on potential toxicity of Laodelphax striatellus, which includes: determining artificial feed effective to a determination system of toxicity on Laodelphax striatellus; developing a technique and a method for adding different organic or inorganic insecticidal compounds into the artificial feed; screening positive comparative compounds (protease inhibitor E-64 or inorganic compound, PA (potassium dihydrogen arsenate)) suitable for the determination system; providing suitable toxicity influence evaluation indexes for Laodelphax striatellus by experimental research; using E-64 and PA as modal compounds; verifying effectiveness and sensitiveness of the built method of toxicity determination for Laodelphax striatellus by dose-reaction bioassay; and finally building the systematic and sensitive method for determining potential toxicity influence of insecticidal compounds on Laodelphax striatellus. The method can be used for evaluating potential toxicity of exogenous insecticidal protein expressed in insect-resistant transgenic crops on Laodelphax striatellus and screening novel insecticidal protein genes resistant to Laodelphax striatellus, and can also be used for determining potential influence of different insecticides on Laodelphax striatellus.

Description

technical field [0001] The invention relates to a method for determining the potential toxicity of an insecticidal compound to the brown planthopper. Background technique [0002] Since the first commercial cultivation of transgenic Bt plants in the United States in 1996, the acreage of transgenic crops in the world has continued to grow rapidly. According to the data released by ISAAA, in 2010, the global planting area of ​​transgenic crops reached 148 million hectares (James, 2010), of which 58.6 million hectares of transgenic crops with insect resistance properties accounted for 37.5% of the total planting area. The planting of transgenic Bt insect-resistant plants can effectively reduce the use of broad-spectrum chemical pesticides, reduce the pollution of pesticides to the environment, and protect human health. [0003] The use of transgenic insect-resistant plants is a huge green revolution in crop pest control technology. However, like other pest control measures, w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50
Inventor 林克剑王增霞李云河刘泽文彭于发
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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