A sea cucumber polypeptide with the activity of inhibiting the proliferation of macrophages and its application

A technology of sea cucumber polypeptides and macrophages, which is applied in the field of biomedicine and can solve the problems of few reports on its effects

Active Publication Date: 2022-07-15
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the inventors have found that up to now, there are few reports on the effect of sea cucumber polypeptides on macrophages

Method used

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  • A sea cucumber polypeptide with the activity of inhibiting the proliferation of macrophages and its application
  • A sea cucumber polypeptide with the activity of inhibiting the proliferation of macrophages and its application
  • A sea cucumber polypeptide with the activity of inhibiting the proliferation of macrophages and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0122] Example 1 P1 (polypeptide sequence 5'-3' is WDGVGVSGR, SEQ ID NO.1) Study results of sea cucumber synthetic peptide on macrophage proliferation rate

[0123] The effect of P1 on the proliferation activity of macrophages was detected by MTT method. The results of five parallel experiments showed that P1 had obvious biological activity. The value-added rates were 23.13%, 39.52%, and 57.39%, respectively. In the t-test test, P<0.001, there is a very significant difference. This indicates that P1 has a significant inhibitory effect on RAW264.7 macrophages.

experiment example 1

[0124] Experimental Example 1 The research results of P2 (polypeptide sequence 5'-3' is WVDGVGSGR, SEQ ID NO. 2) participating in the synthetic peptide on the proliferation rate of macrophages

[0125] The effect of P2 on the proliferation activity of macrophages was detected by MTT assay. The experimental results are as image 3 As shown, there was no significant difference by t-test, indicating that P2 has no biological activity on macrophages.

experiment example 2

[0126] Experimental Example 2 P3 (polypeptide sequence 5'-3' is WDVGVGSGR, SEQ ID NO.3) Study results of sea cucumber synthetic peptide on cell proliferation rate

[0127] The effect of P3 on the proliferation activity of macrophages was detected by MTT assay. The experimental results are as Figure 4 As shown, there was no significant difference by t-test, and P3 had no biological activity on macrophages.

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Abstract

The invention provides a sea cucumber polypeptide with the activity of inhibiting the proliferation of macrophages and its application, belonging to the technical field of biomedicine. Tests show that the artificially synthesized sea cucumber polypeptide of the present invention has an obvious inhibitory effect on the proliferation of macrophages, and presents an obvious dose-effect relationship. Cell proliferation-related diseases, and can also be used as a macrophage proliferation inhibitor to inhibit the in vitro culture of macrophages, thereby providing materials for macrophages and related scientific research. Therefore, it has a good prospect of practical application.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a sea cucumber polypeptide with the activity of inhibiting the proliferation of macrophages and its application. Background technique [0002] The information disclosed in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] Sea cucumbers, belonging to the class of Holothuroidea, are marine echinoderms that live from the sea to 8,000 meters and have a history of more than 600 million years. Sea cucumber is as famous as ginseng, bird's nest and shark's fin, and is one of the eight treasures in the world. Sea cucumber is not only a precious food, but also a precious medicinal material. According to the "Compendium of Materia Medica Su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C12N15/12A61K38/08A61P35/00A61P9/00A61P9/10A61P13/12
CPCC07K7/06A61P35/00A61P9/00A61P9/10A61P13/12A61K38/00
Inventor 宋淑亮吉爱国
Owner SHANDONG UNIV
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