Diglucoside, and preparation method and application thereof
A technology of disglycoside and extract, which is applied in the field of disoside and its preparation and use, and can solve problems such as no disoside and the like.
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Embodiment 1
[0030] (1) Take the serum powder, extract twice with 95% ethanol at 85°C under reflux, and recover the ethanol to obtain the extract;
[0031] (2) After suspending the obtained extract by adding an equal volume of distilled water, extract three times with petroleum ether, diethyl ether, ethyl acetate, and n-butanol, which are equal to the volume of the suspension, respectively, take the n-butanol part, and recover n-butanol under reduced pressure. butanol extract;
[0032] (3) Take the extract obtained in step (2) and add an equal volume of distilled water, then apply D101 macroporous adsorption resin, and perform gradient elution with ethanol with a concentration of 0%, 30%, 60%, and 90% for 3 times the column volume, respectively. , recovering 60% of the site extract, and then through silica gel short column chromatography, respectively, with different ratios of chloroform-methanol gradient elution, chloroform: methanol (15:1) to obtain the compound.
Embodiment 2
[0034] (1) Take the coarse powder of serum, extract it three times with 65% ethanol at 85°C, and recover the ethanol to obtain the extract;
[0035] (2) Suspend the obtained extract by adding an equal volume of distilled water, extract twice with petroleum ether, diethyl ether, ethyl acetate, and n-butanol, respectively, take the fraction of n-butanol, and recover under reduced pressure n-butanol extract;
[0036] (3) Take the extract obtained in step (2) and add an equal volume of distilled water, then apply D101 macroporous adsorption resin, and use 3.5 times the column volume of ethanol with a concentration of 0%, 30%, 60%, and 90% for gradient elution , recovering 60% of the site extract, and then through silica gel short column chromatography, respectively, with different ratios of chloroform-methanol gradient elution, chloroform: methanol (15:1) to obtain the compound.
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