Supercharge Your Innovation With Domain-Expert AI Agents!

Monoclonal anti-soybean lipoxygenase Lox2 antibody hybridoma cell strain and antibody and application thereof

A hybridoma cell line, monoclonal antibody technology, applied in biochemical equipment and methods, anti-enzyme immunoglobulin, microorganism-based methods, etc., can solve problems such as sensitivity and specificity limitations

Active Publication Date: 2013-04-17
河北省农林科学院粮油作物研究所
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since it is determined by the principle of color reaction of lipoxygenase coupled redox indicator, its sensitivity and specificity are limited to a certain extent.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Monoclonal anti-soybean lipoxygenase Lox2 antibody hybridoma cell strain and antibody and application thereof
  • Monoclonal anti-soybean lipoxygenase Lox2 antibody hybridoma cell strain and antibody and application thereof
  • Monoclonal anti-soybean lipoxygenase Lox2 antibody hybridoma cell strain and antibody and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1: Establishment of a hybridoma cell line that secretes monoclonal antibodies against soybean lipoxygenase isozyme

[0075] 1.1 Experimental materials:

[0076] Soybean lipoxygenase all-deficient material "Zhongte No. 1" was purchased from the Institute of Crop Science, Chinese Academy of Agricultural Sciences;

[0077] The preservation numbers of the National Resource Bank of Century near-isogenic materials in the United States are: Lox-1: WDD01483, Lox-3: WDD01485;

[0078] Soybean variety "Wuxing 2" lacking Lox-2 enzyme, registration number: National Approval Bean 2004005;

[0079] BALB / C mice and mouse SP2 / 0 cells were purchased from the Experimental Animal Center of the Academy of Military Medical Sciences (Beijing);

[0080] New Zealand White Rabbit: purchased from Hebei Experimental Animal Center;

[0081] The 96-well polystyrene microplate was purchased from Xiamen Yunpeng Technology Development Co., Ltd.

[0082] 1.2 Chemical reagents:

[0083] HAT medium, Freund's c...

Embodiment 2

[0107] Example 2: Preparation, detection and application of monoclonal antibodies

[0108] 2.1 Preparation of detection antibody

[0109] 2.1.1 Monoclonal antibody preparation and purification

[0110] Monoclonal antibody ascites preparation: the cell line Lox1-E obtained in step ⑧ in Example 1 1 , Lox2-G 5 , Lox3-H 8 Use culture flasks to expand the culture to 1×10 6 The amount of cells / mouse was inoculated into the abdominal cavity of BALB / C mice pretreated with paraffin oil to induce ascites production. Each cell line was inoculated with 5 mice. Ascites can be collected after 7 days. Lox1-E 1 , Lox2-G 5 , Lox3-H 8 Get the monoclonal antibody ascites 25ml, 19ml, 23ml respectively.

[0111] Monoclonal antibody ascites titer determination: indirect ELISA method, Sigma product Soybean Lox type1-B as the detection antigen, determination of Lox1-E 1 , Lox2-G 5 , Lox3-H 8 The titers of monoclonal antibody ascites were 1:320,000, 1:640,000, and 1:320,000 respectively.

[0112] Purification o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a monoclonal anti-soybean lipoxygenase Lox2 antibody hybridoma cell strain and an antibody and the application thereof, which belong to the technical field of biological detection. The preservation number of the monoclonal anti-soybean lipoxygenase Lox2 antibody hybridoma cell strain provided by the invention is CGMCC5122. A monoclonal antibody secreted by the hybridoma cell strain provided by the invention can realize the specificity detection of soybean lipoxygenase Lox2. The invention further provides the monoclonal antibody secreted by the hybridoma cell strain and the application of the antibody in the detection of the soybean lipoxygenase Lox2.

Description

[0001] Division description [0002] This application is a divisional application of a monoclonal antibody hybridoma cell line with the application date of August 2, 2011, the application number of 201110219334.4, and the invention name of soybean lipoxygenase isozyme, and its antibody and application patent applications. Technical field [0003] The present invention relates to a monoclonal antibody hybridoma cell line and its monoclonal antibody, in particular to a monoclonal antibody hybridoma cell line that secretes anti-soy lipoxygenase isoenzymes Lox1, Lox2 and Lox3 and its monoclonal antibody, respectively The deposit numbers of the strains are: CGMCC5121, CGMCC5122, and CGMCC5123. They also refer to the application of the monoclonal antibodies secreted by each of them in the detection of soybean lipoxygenase isoenzymes by the double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), which belongs to biological detection. Technical field. Background technique ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/20C07K16/40G01N33/577C12R1/91
Inventor 张孟臣邸锐杨春燕赵双进蒋春志闫龙马志民史晓蕾
Owner 河北省农林科学院粮油作物研究所
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com