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Method for control of phytophthora blight of pepper by mixed application of trichoderma preparation and fungicides

A fungicide, Trichoderma technology, applied in the directions of fungicides, botanical equipment and methods, chemicals for biological control, etc. The effect of sustainable agricultural development promotion, easy operation and reasonable design

Active Publication Date: 2013-04-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a method for preventing and treating pepper blight by mixing Trichoderma preparations and fungicides to solve the problem that simple biological control of pepper blight cannot control the prevalence of the disease, and at the same time reduce the use of chemical agents

Method used

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  • Method for control of phytophthora blight of pepper by mixed application of trichoderma preparation and fungicides
  • Method for control of phytophthora blight of pepper by mixed application of trichoderma preparation and fungicides
  • Method for control of phytophthora blight of pepper by mixed application of trichoderma preparation and fungicides

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Experimental program
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Effect test

Embodiment 1

[0043] The preparation of embodiment 1 Trichoderma preparation

[0044] (1) Preparation of Trichoderma aculeatus Thz01 spore suspension: inoculate Trichoderma aculeatus Thz01 into PDA medium, and cultivate it for 5-6 days at 25°C; after the cultivation is completed, scrape the spores into sterilized water to prepare Trichoderma aculeatus Thz01 spore suspension (viable bacteria concentration is 10 8 individual / mL).

[0045] (2) Preparation of Trichoderma preparation: inoculate the spore suspension of Trichoderma aculeatus Thz01 into cooked and sterilized wheat grains (feed wheat grains), stir in time during the cultivation period, and alternately cultivate for 18 days after 12 hours of light and 12 hours of darkness , a large number of spores are produced on the induction medium, and the spores are prepared into a Trichoderma preparation together with the spores.

[0046] The dosage of Trichoderma aculeatus Thz01 spore suspension is 3 mL per kilogram of wheat kernels.

Embodiment 2

[0047] The screening of embodiment 2 fungicides

[0048] Select 13 kinds of fungicides commonly used in the production to prevent and treat pepper blight for screening tests, including: 80% aluminum triethylphosphonate (Zhejiang Jiahua Chemical Co., Ltd.); 250g / L mandipropamid (Swiss Syngenta Protection Co., Ltd.); 250g / L Amisida (Syngenta Co., Ltd., UK); 72% Cymoxanil Mancozeb (DuPont, USA); 50% Dimethomorph (Shijiazhuang Yinuo Biochemical Co., Ltd.); 69% enoyl manganese zinc (Kalilong Biochemical Co., Ltd.); 72% Ruifu mold (Jiangsu Kailier Biochemical Company); 50% ethyl aluminum manganese zinc (Limin Chemical Co., Ltd.); 46% copper hydroxide (U.S. DuPont Company) ; 75% chlorothalonil (Limin Chemical Co., Ltd.); 0.3% eugenol (Baoding Yada Chemical Co., Ltd.); 20% flumorph (Shenyang Kechuang Chemical Co., Ltd.); Moldcarb (Bayer CropScience, Germany).

[0049] Drug screening is divided into the following three processes:

[0050] (1) Inhibitory effect of different fungicide...

Embodiment 3

[0080] Embodiment 3 trichoderma preparation and fungicide field mixed use screening test

[0081] Firstly, the field plots were divided, and the blank control area, the chemical control treatment area and the Trichoderma-medicine mixed treatment area were respectively set up. When the pepper is planted, the pepper seedlings and the Trichoderma preparation are transplanted into the planting hole in the Trichoderma-medicine mixed treatment area, and 1.5-2 grams are applied to each plant; the blank control area and the control agent area do not apply the Trichoderma preparation. One month later, the Phytophthora spore suspension was inoculated with the root irrigation inoculation method, and the concentration of the suspension was 107 / ml, and every pepper was inoculated with 3ml. Three days after inoculation, spray fungicides in the Trichoderma-medicine mixed treatment area according to the following 6 application rate gradients: mandipropamid 6g / mu, 6.6g / mu, 7.2g / mu, 7.8g / mu, 8....

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Abstract

The present invention discloses a method for control of phytophthora blight of pepper by mixed application of trichoderma preparation and fungicides. The method comprises: seeding after mixing pepper seeds with the trichoderma preparation during seeding, or, applying the trichoderma preparation during pepper seedling transplanting and planting; during pepper cultivation, applying the fungicides before the occurrence of the disease or in early days of the disease; wherein the active strain in the trichoderma preparation is Trichodermaasperellum Thz01, and the preservation number thereof is CGMCC No. 6422; the fungicides are one or both of eugenol and mandipropamid. The trichoderma preparation used in the method of the invention has good control effect on phytophthora blight of pepper in the fields, and can effectively reduce the primary infection source when being used alone; while the additional application of the fungicides can effectively control disease epidemics. According to the method of the invention, the application amount of the fungicides is reduced, so residues of pesticides in the soil and pepper products are decreased, thereby ensuring environmental and product safety.

Description

technical field [0001] The invention relates to the technical field of biological control and chemical control of plant diseases, in particular to a method for preventing and treating pepper blight by mixing a trichoderma preparation and a fungicide. Background technique [0002] Pepper blight, commonly known as "dead seedling disease", is a soil-borne disease caused by Phytophthora capsici. It is a devastating disease that can cause serious yield losses. Because the pathogen produces a large number of zoospores during the epidemic period, it can quickly cause a large area of ​​pepper to die in a short period of time. The conventional prevention and control technology of pepper blight has been difficult to effectively control, and it is easy to cause ecological and environmental problems. [0003] The invention patent application with the publication number CN102415378A discloses a fungicide and its application that can promote growth and prevent and treat pepper blight. 1...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N37/36A01N31/16A01P3/00
Inventor 张敬泽张艳丽蒋恒
Owner ZHEJIANG UNIV
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