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Method for inducing proliferation and differentiation of muscle mesenchymal stem cells by medicines, and application

A technology of stem cells and astrocytes, applied in the field of biomedicine, can solve the problem of unresearched ginkgolide B dose-effect relationship and other issues

Inactive Publication Date: 2013-05-08
北京清美联创干细胞科技有限公司
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Problems solved by technology

Whether ginkgolide B can promote the differentiation of nerve-stem cells into neurons has not been officially reported at home and abroad, let alone whether there is a dose-effect relationship between the effects of ginkgolide B

Method used

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  • Method for inducing proliferation and differentiation of muscle mesenchymal stem cells by medicines, and application

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Embodiment Construction

[0018] The methods for culturing muscle mesenchymal stem cells and differentiated cells in vitro are as follows:

[0019] Newborn SD rats were selected, and the leg muscles were taken after decapitation, cut into pieces to make a suspension of isolated cells, and the isolated cells were inoculated into cell culture bottles / culture plates, and placed in an incubator for culture. The differential speed adherence culture method was adopted, the medium was changed for the first time after 3 days, and the muscle mesenchymal stem cells formed by the primary culture were subcultured after 10 to 14 days. After the second passage was cultured for 6-7 days, some cells were taken for identification. Muscle mesenchymal stem cells of the second-generation clones were collected and seeded in 24-well culture plates. The experiment was divided into four groups: control group, 20ug / ml ginkgolide B (Shanghai Yongye Biotechnology Co., Ltd., 90164) group, 40ug / ml ginkgolide B group and 60ug / ml g...

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Abstract

The invention relates to a method for inducing proliferation and differentiation of muscle mesenchymal stem cells by medicines, and an application. The in-vitro muscle mesenchymal stem cells are induced to proliferate into neuron cells and astrocytes under the action of ginkgolide B. The method demonstrates that the ginkgolide B can induce the muscle mesenchymal stem cells to proliferate into neuron cells at a cellular level and play an important role in participating reconstruction of neural network and repairing functions of a central nervous system.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method and application for drug-induced proliferation and differentiation of muscle mesenchymal stem cells. Background technique [0002] Muscle mesenchymal stem cells are precursor cells found in adult skeletal muscle tissue to rebuild muscle tissue after trauma. The research on skeletal muscle myoblasts is earlier and there are many animal and clinical studies. Myoblasts are easy to identify, culture, and propagate, making them an excellent choice for clinical use. Myoblasts have multilineage differentiation potential, can differentiate into adipocytes in vitro, and have osteogenic ability. Whether rat myoblasts can transdifferentiate into neurons is rarely reported at home and abroad. [0003] Ginkgo biloba extract (Extract of Ginkgo bilobo, EGb) is a mixture obtained from the leaves of Ginkgo biloba plants through multi-step separation and purification. It is known t...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12N5/0793C12N5/079
Inventor 田杰
Owner 北京清美联创干细胞科技有限公司
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