55 results about "Neural cell differentiation" patented technology

Cyclic glycerophosphates and analogs thereof (CGs) are shown to exert neutral promoting activities in target cells. Such activities include promotion of neuronal outgrowth, promotion of nerve growth, provision of dopaminotrophic supporting envrionment in a diseased portion of the brain, prevention of nerve degeneration and nerve rescue. These activities of the CGs render them useful for treatment of various disorders including but not limited to mental disorders such as, for example, schizophrenia, dementia or disorders resulting in learning disablities. In addition, these CGs may be used for the treatment of neurodegenerative conditions such as Altzheimer's diesease, Parkinson's disease, conditions resulting from exposure to harmful environmental factors or resulting from a mechanical injury. The CGs may also be used to treat an individual suffering from a primary neurodengenerative condition in order to prevent or reduce the appearance of secondary degeneration in additional nerves (“nerve rescue”).

The invention relates to a method for inducing mouse embryonic stem cells (mESCs) to differentiate toward nerve cells. Primary mouse embryonic fibroblasts (PMEF) are used as a feeder layer. The method comprises the following steps of: inoculating the mESCs to the feeder layer, inoculating the mESCs to mixed solution of stem cells and mESCs culture liquid, and culturing nerve stem cell culture solution; and gradually changing the culture into the culture of nerve cell culture solution by completely using serum-free added alkali fibroblast growth factors, wherein the optimal serum concentration of the mixed solution for primary inoculation is 12.5 percent, the optimal inoculation density of primary mESCs inoculation is 1.0*108<-1>, and the time of completely changing the serum-free culture solution is the 5th day. By induction of the method, the mouse stem cells can be differentiated into the nerve cells in vitro. The method has the advantages of short experimental period, simple and convenient experimental process and stable and efficiency inducing results, can quickly finish a cell culture period, and is favorable for subsequent experiments.

The invention discloses a dental pulp stem cell exosome preparation as well as a preparation method and application thereof, and relates to the technical field of biology. Dental pulp stem cells can express neuronal cell surface markers, can be differentiated to nerve cells and promote the proliferation of the nerve cells, so that the dental pulp stem cells can be used for preparing cell preparations for repairing nerve injuries such as traumatic brain injuries (TBI). The dental pulp stem cells are used for replacing traditional embryonic stem cells, neural stem cells or bone marrow stem cellsto be used for treating the nerve injuries, and have the advantages of being rich in source, simple and convenient to obtain, low in immunogenicity and capable of avoiding ethical problems. Dental pulp stem cell exosomes can accurately target recipient cells and are used for preparing nerve repair preparations for the injuries such as the TBI, and then treatment of the nerve injuries such as theTBI by the exosomes is achieved. The dental pulp stem cell exosomes can also be used for preparing neuroinflammation inhibitors.

The present invention is method of inducing differentiation of human human amnion mesenchyme stem cell to nerve cell. The method is to culture human human amnion mesenchyme stem cell in DMEM / F12 culture medium containing all transcofiguration vitamin A acid, basic fibroblast growth factor and ox embryo blood serum. Through the induction of the method, human human amnion mesenchyme stem cell may be differentiated extracorporeally into neure cell. The nerve cell of the present invention and composition containing the nerve cell may be used widely in treating neurogenic diseases.

A use of a composition comprising umbilical cord blood-derived mesenchymal stem cells for inducing differentiation and proliferation of neural precursor cells or neural stem cells to neural cells is provided, the composition being effective for the treatment of nerve injury diseases.

The present invention provides a nerve cell differentiation inducing drug containing a Synoviolin expression inhibitor which is a useful drug for treatments of neural disorders, particularly Alzheimer's disease, Parkinson's disease, peripheral nerve disorders or spinal injury. As Synoviolin expression inhibitors, there are siRNA against gene coding Synoviolin (SEQ ID No. 1 or 2), or shRNA, a decoy nucleic acid that inhibits the promoter activity by binding to the transcription factor of the promoter of the Synoviolin gene or antisense oligonucleotide against gene coding the Synoviolin. The nerve cell differentiation inducing drug of the present invention is used for the treatment of neural disorders including Alzheimer's disease, Parkinson's disease, peripheral nerve disorders or spinal injury.

The invention discloses an application of lncRNA (long noncoding RNA) TUNAR to regulation of cerebral nervous system development. Signaling pathways, such as a nervous system development pathway and the like closely related to nervous system development molecular functions, as well as 319 target genes, related to prognosis of nervous system development, of long noncoding TUNAR are predicted successfully through co-expression analysis on the basis of a GEO chip common data base of NCBI (National Center of Biotechnology Information) in the USA. Further, RNA-seq sequencing results show high expression of both TUNAR and related nervous system proteins in nervous system specimens, and the correlation is quite remarkable. On the basis of the discovery, the mechanism of TUNAR induced cerebral dysplasia is expected to be determined clearly, a molecular regulation network based on neural cell differentiation is established gradually, and a theoretical basis is provided for searching new targets of prevention and drug treatment of cerebral dysplasia.

The invention discloses a ganoderma lucidum extract, a preparation method and application thereof. The Ganoderma lucidum extract can be prepared by the following method: taking the broken spore powder of Ganoderma lucidum as raw material, degreasing with petroleum ether, and volatilizing until there is no ether smell; refluxing the obtained powder with ethanol for extraction, and concentrating the extract to extract. Further, the obtained extract can be made into powder, and the solid matter extracted with chloroform is then extracted with ethyl acetate, and the extract is recovered under reduced pressure and dried to obtain powdered Ganoderma lucidum extract. The extract can effectively induce nerve cell differentiation, and can be used for preparing medicines for treating nerve injury and neurodegeneration.

The invention provides calcium silicate nano hollow microsphere modified thermosensitive hydrogel and a preparation method and application thereof. The preparation method includes: preparing calcium silicate nano hollow microspheres, dispersing the microspheres in the complex solution of carboxymethyl chitosan and beta-sodium glycerophosphate, and processing at 30-70 DEG C. The preparation methodhas the advantages that the method is simple and low in cost, the reaction conditions of the method can be achieved easily, and the raw materials used by the method are cheap and easy to obtain; the prepared thermosensitive hydrogel is safe, low in cytotoxicity, good in biocompatibility and good in low-temperature flowability, the hydrogel can fast turn into gel at 37 DEG C, the surface of the gelis loose and porous, and the gel is large in specific surface and capable of benefiting neural stem cell adsorption; the calcium silicate nano hollow microspheres are evenly dispersed in the thermosensitive hydrogel, and the thermosensitive hydrogel can promote neural stem cell differentiation; when the thermosensitive hydrogel is applied to brain tissue damage repairing, neural stem cell adsorption can be promoted effectively, the neural stem cells can be effectively promoted to differentiate into neurons and neuroglia, and the thermosensitive hydrogel is promising in clinical application prospect.

The invention relates to an acylhydrazone compound and a preparation method and the application thereof. The acylhydrazone compound has the following structure as shown in a formula, wherein, R is selected from one of the two parts of the formula. The acylhydrazone compound can promote neural cell differentiation and neurite growth.

Cyclic glycerophosphates and analogs thereof (CGs) are shown to exert neutral promoting activities in target cells. Such activities include promotion of neuronal outgrowth, promotion of nerve growth, provision of dopaminotrophic supporting envrionment in a diseased portion of the brain, prevention of nerve degeneration and nerve rescue. These activities of the CGs render them useful for treatment of various disorders including but not limited to mental disorders such as, for example, schizophrenia, dementia or disorders resulting in learning disablities. In addition, these CGs may be used for the treatment of neurodegenerative conditions such as Altzheimer's diesease, Parkinson's disease, conditions resulting from exposure to harmful environmental factors or resulting from a mechanical injury. The CGs may also be used to treat an individual suffering from a primary neurodengenerative condition in order to prevent or reduce the appearance of secondary degeneration in additional nerves ("nerve rescue").

The invention relates to an unsaturated fatty acid compound as well as a preparation method and application thereof. According to the invention, the fatty acid compound is separated and identified from an ethyl acetate cold-soaked extract of dry root of alchornea rosea by adopting multiple chromatographic separation means and a spectrum-wave spectrum technology, and activity screening is performed through a PC12 cell (rat adrenal medullary pheochromoma differentiated cell strain) model to obtain the new unsaturated fatty acid compound with the function of enhancing nerve cell differentiation.

Differentiation of mesodermal stem cells into nervous system cells can be induced by immortalizing the mesodermal stem cells by overexpressing or activating an immortalization gene therein and then culturing these cells under appropriate conditions. This method is highly useful in nerve regeneration therapy.

The invention relates to an ESC (embryonic stem cell) culture medium and an application thereof. The culture medium contains one differential inhibition short-chain polypeptide capable of inhibiting differentiation of ESCs, wherein the short-chain polypeptide can inhibit differentiation of the ESCs to nerve cells. The culture medium has the advantages of being rapid in multiplication, safe, stableand long in passage time when used for multiplication culture on the ESCs; and a large quantity of passaged and multiplied ESCs can be obtained, the obtained ESCs can keep an undifferentiated state,and the totipotency of cell differentiation can be kept.