56 results about "Neural cell differentiation" patented technology

There is provided a treatment for patients suffering from neurodegenerative disease or neural injury including the steps of transplanting cultured bone marrow cells into the spinal cord or brain or injecting intravascularly bone marrow cells of a patient in need. Also provided is a method of activating the differentiation of neural cells in an injured brain including the steps of transplanting bone marrow cells adjacent to the injured brain cells and activating the endogenous central nervous system stem cells to differentiate into neurons. A method of treating injured brain or spinal cord cells is also provided including the steps of transplanting bone marrow cells near the injured brain cells and generating new neurons at the location of transplantation. A method of treating injured brain or spinal cord cells with a composite of MSCs and neurospheres.

Cyclic glycerophosphates and analogs thereof (CGs) are shown to exert neutral promoting activities in target cells. Such activities include promotion of neuronal outgrowth, promotion of nerve growth, provision of dopaminotrophic supporting envrionment in a diseased portion of the brain, prevention of nerve degeneration and nerve rescue. These activities of the CGs render them useful for treatment of various disorders including but not limited to mental disorders such as, for example, schizophrenia, dementia or disorders resulting in learning disablities. In addition, these CGs may be used for the treatment of neurodegenerative conditions such as Altzheimer's diesease, Parkinson's disease, conditions resulting from exposure to harmful environmental factors or resulting from a mechanical injury. The CGs may also be used to treat an individual suffering from a primary neurodengenerative condition in order to prevent or reduce the appearance of secondary degeneration in additional nerves (“nerve rescue”).

The invention belongs to the field of biomedicine, and relates to a method for differentiation of embryonic stem cells into nerve cells through in vitro induction, especially to a method for induction of Olig2<+>-GFP<+>-mES nerve cell differentiation through a purine derivative Purmorphamine, and uses thereof. According to the present invention, an embryoid body mediated nerve induction method is adopted, Olig2-GFP<+>-mES is adopted as a cell model, a purine derivative Purmorphamine and all-trans retinoic acid are combined to carry out directed induction on the Olig2-GFP<+>-mES cells to obtain spinal motor neurons and oligodendrocyte progenitor cells through differentiation; experiment results show that the Purmorphamine can be used as a substitute of SHH, can effectively induce Olig2<+>-GFP<+>-mES differentiation to obtain high purity and function spinal motor neurons and oligodendroglial cells, and can cause expression changes of related genes; and transplant experiment results show that the induced nerve cells can promote partial function and morphology restoration after rat spinal cord injury, and have effects of spinal cord injury regeneration promotion and function reconstruction.

The invention provides a kit and a method for in-vitro directionally differentiating human-derived iPS stem cells into nerve cells. By utilizing a culture solution provided by the kit and according to the method, the iPS stem cells can be efficiently and directionally differentiated into the nerve cells. The kit and the method are simple, reliable, stable, efficient and high in safety, proportion of differentiating pluripotent stem cells into nerve cells is obviously increased, and differentiating proportion of the pluripotent stem cells is higher than 90%. In addition, the kit and a culture medium are suitable for large-scale production of high-quality nerve cells and can be directly used for scientific research on neurodevelopment, cell therapy of nerve diseases, nerve injury repair and application of drug screening without subsequent screening and purifying.

The invention discloses a method for preparing a sub totipotential stem cell by a utilizing placenta lobular tissue. The method is characterized by comprising the following steps of: preparing cell suspension by utilizing the placenta lobular tissue after pretreating placenta, and preparing sub totipotential stem cell separating medium by utilizing the cell suspension; and carrying out primary culture and a second-generation culture on the sub totipotential stem cell separating medium, and collecting fully cast-off cells in the second-generation culture process to obtain the sub totipotential stem cell; and cooling and freezing in a programmed manner, taking out the frozen sample after cooling the temperature to -80 DEG C to -90.0 DEG C, and placing the frozen sample in a liquid-nitrogen storage tank for storing for a long time. The method for preparing the sub totipotential stem cell by utilizing the placenta lobular tissue has the advantages that the passage capacity can reach 20 generations, so that not only the capacity of differentiating towards bones, cartilages, fat and nerve cells can be achieved, but also the capacity of differentiating towards islet cells and epidermis cells be achieved; and the sub totipotential stem cell is uniform, stable, short in production period, simple to operate and low in cost.

The invention relates to a method for inducing mouse embryonic stem cells (mESCs) to differentiate toward nerve cells. Primary mouse embryonic fibroblasts (PMEF) are used as a feeder layer. The method comprises the following steps of: inoculating the mESCs to the feeder layer, inoculating the mESCs to mixed solution of stem cells and mESCs culture liquid, and culturing nerve stem cell culture solution; and gradually changing the culture into the culture of nerve cell culture solution by completely using serum-free added alkali fibroblast growth factors, wherein the optimal serum concentration of the mixed solution for primary inoculation is 12.5 percent, the optimal inoculation density of primary mESCs inoculation is 1.0*108<-1>, and the time of completely changing the serum-free culture solution is the 5th day. By induction of the method, the mouse stem cells can be differentiated into the nerve cells in vitro. The method has the advantages of short experimental period, simple and convenient experimental process and stable and efficiency inducing results, can quickly finish a cell culture period, and is favorable for subsequent experiments.

The invention discloses a glossy ganoderma extract, its preparation method and use, wherein the glossy ganoderma extract is prepared through the following steps, using wall-broken glossy ganoderma spore powder as raw material, degreasing with petroleum ether, volatilizing, extracting the powder by ethanol backflow, concentrating the extract to concrete form, preparing powder form from the obtained concrete substance, chloroform extracting solid body, extracting again with acetic acid ethyl ester, decompressing and drying the extract. The extract can be used for preparing the medicament for treating nerve damage and nerve retrogression pathology.

The invention discloses a dental pulp stem cell exosome preparation as well as a preparation method and application thereof, and relates to the technical field of biology. Dental pulp stem cells can express neuronal cell surface markers, can be differentiated to nerve cells and promote the proliferation of the nerve cells, so that the dental pulp stem cells can be used for preparing cell preparations for repairing nerve injuries such as traumatic brain injuries (TBI). The dental pulp stem cells are used for replacing traditional embryonic stem cells, neural stem cells or bone marrow stem cellsto be used for treating the nerve injuries, and have the advantages of being rich in source, simple and convenient to obtain, low in immunogenicity and capable of avoiding ethical problems. Dental pulp stem cell exosomes can accurately target recipient cells and are used for preparing nerve repair preparations for the injuries such as the TBI, and then treatment of the nerve injuries such as theTBI by the exosomes is achieved. The dental pulp stem cell exosomes can also be used for preparing neuroinflammation inhibitors.

The present invention relates to a method for differentiation of mesenchymal stem cells or dental pulp stem cells. More specifically, the invention relates to a method for differentiating stem cells to neural cells by applying mesenchymal stem cells or dental pulp stem cells with a low-frequency electromagnetic field. The differentiation method according to the present invention can induce differentiation even with low-cost mediums rather than induced neural differentiation mediums which are expensive due to addition of growth factors, and the neural cells differentiated according to the present invention may be useful for treatment of neurological brain diseases.

The present invention provides methods for promoting or suppressing differentiation of neural stem / progenitor cells, for producing differentiated neural cells, and for isolating / purifying differentiated neural cells. Also provided are differentiated neural cells, cell populations and transgenic animals comprising same, and uses of same. The present invention further provides methods for treating nervous tissue degeneration, and for identifying an agent for use in treating nervous tissue degeneration. Additionally, the present invention provides a therapeutic composition, and methods for treating neural tumors using same. The present invention further provides methods for identifying agents which inhibit ATF5, agents identified by these methods, and uses of same. Also provided are methods for diagnosing neural tumors, for assessing the efficacy of therapy to treat neural tumors, and for assessing the prognosis of a subject who has a neural tumor. Finally, the present invention provides a kit for use in detecting a neural tumor.

The present invention relates to a method for preparing induced dopaminergic neuronal progenitors (iDPs) comprising inducing an expression of Oct4, Sox2, Klf4, and c-Myc genes in adult cells and direct reprogramming of the adult cells to the iDPs by treating the cells with sonic hedgehog (SHH) and fibroblast growth factor 8 (FGF8); and a cell therapy product and a composition for treating or preventing Parkinson's Disease (PD) which comprises the iDPs as active ingredients. In addition to this, the present invention relates to a method for preparing midbrain dopaminergic neurons comprising isolating NSC-like colony by culturing the iDPs, dissociating the isolated NSC-like colony, and culturing the cells on the neural cell differentiation medium. Further, the present invention relates to a method for treating PD or a method for screening medicine for preventing or treating PD using the cells of the present invention. The progenitors prepared by the method for preparing the iDPs of the present invention can be widely used in the known art since the neuronal progenitors have significantly higher efficiency in differentiating to the dopaminergic neurons compared to conventional methods and lack side effects such as immunogenicity and ethical issues because patients' own somatic cells can be used.

The present invention is method of inducing differentiation of human human amnion mesenchyme stem cell to nerve cell. The method is to culture human human amnion mesenchyme stem cell in DMEM / F12 culture medium containing all transcofiguration vitamin A acid, basic fibroblast growth factor and ox embryo blood serum. Through the induction of the method, human human amnion mesenchyme stem cell may be differentiated extracorporeally into neure cell. The nerve cell of the present invention and composition containing the nerve cell may be used widely in treating neurogenic diseases.

The invention relates to a method for inducing proliferation and differentiation of muscle mesenchymal stem cells by medicines, and an application. The in-vitro muscle mesenchymal stem cells are induced to proliferate into neuron cells and astrocytes under the action of ginkgolide B. The method demonstrates that the ginkgolide B can induce the muscle mesenchymal stem cells to proliferate into neuron cells at a cellular level and play an important role in participating reconstruction of neural network and repairing functions of a central nervous system.

The invention relates to sericin conductive hydrogel crosslinked by sericin protein. The sericin conductive hydrogel comprises 0.0125-0.0833g / mL sericin protein and 0.01-0.1mg / mL carbon nanotube. The invention further discloses a preparation method of the sericin conductive hydrogel, a scaffold prepared by the sericin conductive hydrogel and application of the scaffold. The sericin conductive hydrogel and the scaffold prepared by the sericin conductive hydrogel have excellent electrical conduction property, syringeability and memory deformation property and have nerve cell differentiation promoting ability, so that the sericin conductive hydrogel and the scaffold are especially applicable to the wound repairing and treatment of tissue, closely related to electrophysiological activities, of the nerves, the heart, the muscles and the like. The sericin conductive hydrogel and the scaffold and slow-release pharmaceutic preparation prepared by the sericin conductive hydrogel can be transplanted into a human body through injection or minimally invasive surgery, the wound and pain, caused by transplantation, of a patient is reduced to the maximum extent, infection risk caused by the transplantation is also lowered, and severe immunoreaction after the scaffold and the slow-release pharmaceutic preparation are transplanted into the human body is avoided.

The present invention provides a nerve cell differentiation inducing drug containing a Synoviolin expression inhibitor which is a useful drug for treatments of neural disorders, particularly Alzheimer's disease, Parkinson's disease, peripheral nerve disorders or spinal injury. As Synoviolin expression inhibitors, there are siRNA against gene coding Synoviolin (SEQ ID No. 1 or 2), or shRNA, a decoy nucleic acid that inhibits the promoter activity by binding to the transcription factor of the promoter of the Synoviolin gene or antisense oligonucleotide against gene coding the Synoviolin. The nerve cell differentiation inducing drug of the present invention is used for the treatment of neural disorders including Alzheimer's disease, Parkinson's disease, peripheral nerve disorders or spinal injury.

The invention discloses an application of lncRNA (long noncoding RNA) TUNAR to regulation of cerebral nervous system development. Signaling pathways, such as a nervous system development pathway and the like closely related to nervous system development molecular functions, as well as 319 target genes, related to prognosis of nervous system development, of long noncoding TUNAR are predicted successfully through co-expression analysis on the basis of a GEO chip common data base of NCBI (National Center of Biotechnology Information) in the USA. Further, RNA-seq sequencing results show high expression of both TUNAR and related nervous system proteins in nervous system specimens, and the correlation is quite remarkable. On the basis of the discovery, the mechanism of TUNAR induced cerebral dysplasia is expected to be determined clearly, a molecular regulation network based on neural cell differentiation is established gradually, and a theoretical basis is provided for searching new targets of prevention and drug treatment of cerebral dysplasia.

A use of a composition comprising umbilical cord blood-derived mesenchymal stem cells for inducing differentiation and proliferation of neural precursor cells or neural stem cells to neural cells is provided, the composition being effective for the treatment of nerve injury diseases.

The invention provides calcium silicate nano hollow microsphere modified thermosensitive hydrogel and a preparation method and application thereof. The preparation method includes: preparing calcium silicate nano hollow microspheres, dispersing the microspheres in the complex solution of carboxymethyl chitosan and beta-sodium glycerophosphate, and processing at 30-70 DEG C. The preparation methodhas the advantages that the method is simple and low in cost, the reaction conditions of the method can be achieved easily, and the raw materials used by the method are cheap and easy to obtain; the prepared thermosensitive hydrogel is safe, low in cytotoxicity, good in biocompatibility and good in low-temperature flowability, the hydrogel can fast turn into gel at 37 DEG C, the surface of the gelis loose and porous, and the gel is large in specific surface and capable of benefiting neural stem cell adsorption; the calcium silicate nano hollow microspheres are evenly dispersed in the thermosensitive hydrogel, and the thermosensitive hydrogel can promote neural stem cell differentiation; when the thermosensitive hydrogel is applied to brain tissue damage repairing, neural stem cell adsorption can be promoted effectively, the neural stem cells can be effectively promoted to differentiate into neurons and neuroglia, and the thermosensitive hydrogel is promising in clinical application prospect.

A method for inducing differentiation of embryonic stem cells into neuronal precursors is provided as well as an assay for neuronal precursor or progenitor cells and a method for identifying agents that inhibit or reduce an increase in neurite degeneration.

The invention belongs to the technical field of medicines and biology, and particularly relates to an extraction method and application of gastrodia elata oligosaccharide GEP-2. The invention provides an extraction method of gastrodia elata oligosaccharide GEP-2. The gastrodia elata oligosaccharide GEP-2 provided by the invention has a similar net-shaped structure for preparing a dissolution assisting platform, and the dissolution state of an extract can be remarkably improved. Meanwhile, the gastrodia elata oligosaccharide GEP-2 also has excellent activity similar to nerve growth factors (NGF), and can induce nerve cell differentiation and promote pheochromocytoma (PC12) differentiation. Tests prove that the gastrodia elata oligosaccharide GEP-2 provided by the invention can effectively improve the differentiation rate of nerve cells.

The invention relates to an acylhydrazone compound and a preparation method and the application thereof. The acylhydrazone compound has the following structure as shown in a formula, wherein, R is selected from one of the two parts of the formula. The acylhydrazone compound can promote neural cell differentiation and neurite growth.

The invention belongs to the technical field of comprehensive utilization of edible and medical fungi, and specifically relates to a novel application of a morel fruiting body polypeptide. At present,the research on the applications of the morel fruiting body polypeptide is not enough, and in order to solve the problem, the invention provides a novel application of the morel fruiting body polypeptide in the preparation of foods or medicines for preventing or treating neurodegenerative diseases. The invention also provides applications of the morel fruiting body polypeptide in promoting neuralcell differentiation and protecting neural cells from oxidation damage caused by oxidation pressure. Specifically, the invention also provides a preparation method of the morel fruiting body polypeptide. For the first time, people find that the morel fruiting body polypeptide can well promote neural cell differentiation and protect the neural cells, is capable of effectively reducing the damage of oxidation pressure to neural cells, and can promote the neural cell differentiation and delay the aging of neural cells. A novel application of a morel fruiting body is provided.

The invention discloses an application of 3beta-cinnamoyl diosgenin in induced differentiation of mesenchymal stem cells. Nestin is one of neural stem cell markers, expressed in early embryonic development; NSE is an acidic protein peculiar to neuron cells; and MAP-2 is a protein mainly existing in cell bodies and dendrites of neuronal cells, and plays an important role in synaptic growth. The Nestin, NSE and MAP-2 are marker proteins of neural cells, and the expression levels of the Nestin, NSE and MAP-2 can reflect the differentiation level of mesenchymal stem cells into neural cells. The differentiation ability of bone marrow mesenchymal stem cells to nerve cells is enhanced after being induced by 3beta-cinnamoyl diosgenin, and the bone marrow mesenchymal stem cells have similar effectsas positive drugs. Therefore, 3beta-cinnamoyl diosgenin can be used to induce differentiation of bone marrow mesenchymal stem cells into nerve cells in vitro, and can be made into commercial differentiation medium.

Cyclic glycerophosphates and analogs thereof (CGs) are shown to exert neutral promoting activities in target cells. Such activities include promotion of neuronal outgrowth, promotion of nerve growth, provision of dopaminotrophic supporting envrionment in a diseased portion of the brain, prevention of nerve degeneration and nerve rescue. These activities of the CGs render them useful for treatment of various disorders including but not limited to mental disorders such as, for example, schizophrenia, dementia or disorders resulting in learning disablities. In addition, these CGs may be used for the treatment of neurodegenerative conditions such as Altzheimer's diesease, Parkinson's disease, conditions resulting from exposure to harmful environmental factors or resulting from a mechanical injury. The CGs may also be used to treat an individual suffering from a primary neurodengenerative condition in order to prevent or reduce the appearance of secondary degeneration in additional nerves ("nerve rescue").

The invention relates to an unsaturated fatty acid compound as well as a preparation method and application thereof. According to the invention, the fatty acid compound is separated and identified from an ethyl acetate cold-soaked extract of dry root of alchornea rosea by adopting multiple chromatographic separation means and a spectrum-wave spectrum technology, and activity screening is performed through a PC12 cell (rat adrenal medullary pheochromoma differentiated cell strain) model to obtain the new unsaturated fatty acid compound with the function of enhancing nerve cell differentiation.

Differentiation of mesodermal stem cells into nervous system cells can be induced by immortalizing the mesodermal stem cells by overexpressing or activating an immortalization gene therein and then culturing these cells under appropriate conditions. This method is highly useful in nerve regeneration therapy.

The present invention provides an apparatus for stimulating an animal cell and recording its physiological signal and methods of making and using thereof. The purpose of the present invention is to provide an apparatus for stimulating an animal cell and recording its physiological signal that is efficient, convenient, and accurate. The apparatus for stimulating an animal cell and recording its physiological signal of the present invention comprises a poor conductive substrate, wherein on at least one surface of the substrate is provided at least one unit conductive polymer layer and at least one good conductive microelectrode.