8 results about "Neural cell differentiation" patented technology

The invention relates to a method for promoting nerve cell proliferation and differentiation based on ultrasonic stimulation and application, and belongs to the technical field of biomedicine. According to the method, under the conditions that the power is 90-110 mW and the frequency is 0.9-1.1 MHz, nerve cells are continuously stimulated for 3 days by ultrasonic waves once per day, and each time lasts for 2-4 min. The purposes of increasing the nerve cell proliferation speed and promoting nerve cell differentiation are achieved only through ultrasonic stimulation under specific conditions for the first time, no other substances are added, non-invasion and non-contact are achieved, and toxic and side effects on cells are low; the nerve cells are not subjected to invasive or modified operation; the method has a wide application prospect only through physical conditions.

The invention relates to the technical field of nerve cell differentiation, and discloses a culture medium for promoting neural differentiation of SH-SY5Y cells. The culture medium disclosed by the invention is prepared from the following components: tazarotene (tazarotene) and a basic culture medium. The culture medium can promote SH-SY5Y cells to present neuron-like differentiation within 5 days, significantly improves the length of neurite and the expression level of neuron markers, and is low in cytotoxicity. The invention provides an efficient, safe and standardized new tool for neural disease in-vitro model construction, drug screening and mechanism research.

Provided is a method for inducing differentiation into nerve cells, the method comprising: a) culturing mesenchymal stem cells for 1-10 days in a stem cell culture medium configured by including a basal medium at 60.0–90.0 vol% and physiological saline at 10.0–40.0 vol%, the culture medium being further supplemented with 1.0–100.0 ng / mL of EGF, 0.2–20.0 ng / mL of FGF-2, 0.2–20.0 ng / mL of PDGF, and 0.5–8.0 mM of magnesium ascorbyl phosphate; b) thereafter, precipitating protein components from the culture solution obtained in step a) using an organic solvent; and c) isolating the precipitate portion and dissolving the same in a physiological saline or a basal medium to obtain a medium comprising a cell-produced protein (CPPs) composition, and seeding, using the medium, the mesenchymal stem cells at a density of 3×103 to 2×104 cells / mL and culturing for 1-3 days, thereby inducing differentiation of the mesenchymal stem cells into nerve cells.

The invention discloses a nutritional composition for synergistically regulating neural stem cells to differentiate into specific nerve cells. The invention provides a nutritional composition. The nutritional composition comprises the following necessary components: a grease component and a sialic acid oligosaccharide component, moreover, in the nutritional composition, the molar ratio of the grease component to the sialic acid oligosaccharide component is (1-5): (0.01-5). The nutritional composition provided by the invention can effectively promote the neural stem cells to differentiate into specific nerve cells and achieve function maturation, especially significantly promote the neural stem cells to differentiate into neurons, and shows a synergistic enhancement effect on the structure and function aspects, and the components have a synergistic effect.

The present invention provides a use of SQB-DNA nanostructures, prepared using DNA origami technology, for inducing differentiation into nerve cells or nerve cell regeneration, or treating nerve damage or neurological diseases. The present invention is the first instance of achieving nerve recovery through precise ligand presentation using DNA origami nanostructures, and the widespread use of SQB as a therapeutic drug for various types of nerve damage and neurological diseases requiring nerve regeneration is expected due to the provision of the present invention.

The application discloses a nerve regeneration guide conduit for promoting nerve cell differentiation and a preparation method and application thereof. The nerve regeneration guide conduit can realize stable and long-acting release of bioactive factors, effectively reduce the risk of tissue adhesion, efficiently induce nerve cell differentiation, promote axon growth and function recovery, and provides a novel and effective solution for nerve regeneration and repair. In addition, the nerve regeneration guide conduit has excellent conductivity, biocompatibility, mechanical adaptability and the like, and therefore has a wide application prospect in the field of nerve regeneration and repair.

The invention relates to the technical fields of molecular biology, developmental biology, cytobiology and medicine, in particular to application of a PPARG gene or / and an expression product thereof in preparation of a medicine for inhibiting hepatocellular carcinoma, a method and the medicine. According to the present invention, the non-nerve cell differentiation-promoting factor PPARG gene or / and the non-nerve cell differentiation-promoting factor PPARG protein is / are adopted to inhibit tumorigenicity and malignant characteristics of liver cancer cells; the invention provides a protein PPARG capable of promoting muscle fiber differentiation, which can promote cancer cells to differentiate into muscle cells, so that the cancer cells lose nerve stemness and further lose tumorigenicity, thereby inhibiting the development process of cancers and treating the cancers. The invention is a novel cancer treatment method developed based on the discovery of the inventor on the essential characteristics of cancer cells, namely the brand-new regularity of the nerve stemness.

The invention relates to the technical fields of molecular biology, developmental biology, cytobiology and medicine, in particular to application of an MYOD1 gene or / and an expression product thereof in preparation of a medicine for inhibiting hepatocellular carcinoma, a method and the medicine. According to the invention, the non-nerve cell differentiation-promoting factor MYOD1 gene or / and protein is / are used for inhibiting tumorigenicity and malignant characteristics of liver cancer cells. The invention provides a protein MYOD1 capable of promoting muscle fiber differentiation, which can promote cancer cells to differentiate into muscle cells, so that the cancer cells lose nerve stemness and further lose tumorigenicity, thereby inhibiting the development process of cancers and treating the cancers. The invention is a novel cancer treatment method developed based on the discovery of the inventor on the essential characteristics of cancer cells, namely the brand-new regularity of the nerve stemness.