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Novel application of morel fruiting body polypeptide

A new application, the technology of Morchella, applied in the field of new uses of Morchella fruiting body polypeptides, can solve problems such as the relationship between neuroprotection of active polypeptides of Morchella mycelium that have not been studied, so as to improve learning and cognitive ability, slow down Effects of aging, promotion of nerve cell differentiation, or protection of nerve cells

Active Publication Date: 2019-03-29
SAAS BIOTECH & NUCLEAR TECH RES INST
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Master's degree thesis "Separation method and antioxidant activity of Morchella mycelium active polypeptide in northern Shaanxi", (Yan'an University, Yao Yao, 2015) discloses the separation method of active polypeptide from Morchella mycelium in northern Shaanxi And anti-oxidation research, research the free radical scavenging activity of morel mycelium active polypeptide, found that morel mycelium polypeptide has a certain scavenging effect on hydroxyl radicals, superoxide anion free radicals and DPPH free radicals, However, this literature only reported that the active polypeptide of Morchella mycelium has antioxidant function, and did not study the relationship between the active polypeptide of Morchella mycelium and neuroprotection.

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  • Novel application of morel fruiting body polypeptide
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  • Novel application of morel fruiting body polypeptide

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preparation example Construction

[0029] The invention provides a method for preparing a morel fruiting body polypeptide extract, comprising the following steps:

[0030] a. Harvest fresh hickory chicks and process them in an oven at 37-45°C for 12-16 hours, so that the moisture content of the dried hickory chicks is less than or equal to 13%. The dried hickory chicks are crushed with a Chinese herbal medicine grinder and passed through a 100-mesh sieve. Sieve to obtain morel fruiting body dry product powder;

[0031]b. According to the material-liquid ratio of 1:10-30, add morel fruiting body dry product powder and PBS buffer solution, centrifuge at 6000r / min after stirring to obtain supernatant, and dialyze the supernatant in a 7500Da dialysis bag for 12 ~14h, collect the dialysate, concentrate, and dry to obtain the dry powder of Morchella sporocarp polypeptide extract;

[0032] c. Dissolve the dry powder of Morchella fruiting body polypeptide extract in sterile water, load the sample on Superdex30increase...

Embodiment 1

[0035] Example 1 Separation and Extraction of Morel Fruiting Body Polypeptide

[0036] Take by weighing 20 g of the dried Morchella sporocarp, pulverize it with a Chinese herbal medicine pulverizer, and pass through a 100-mesh sieve to obtain the Morchella sporocarp dry powder.

[0037] Configure phosphate buffered saline buffer (PBS buffer): Potassium dihydrogen phosphate (KH 2 PO 4 ) 0.27g, disodium hydrogen phosphate (Na 2 HPO 4 ) 1.42g, sodium chloride (NaCl) 8g, potassium chloride (KCl) 0.2g, add about 800mL of deionized water and stir to dissolve, then add concentrated hydrochloric acid to adjust the pH to 7.4, and finally dilute to 1L.

[0038] Add samples according to the ratio of solid to liquid of Morchella dry product / PBS buffer solution 1:20. After adding the sample, stir with a magnetic stirrer at 120 rpm overnight, take the supernatant, and centrifuge at 6000r / min to obtain the supernatant. Select a dialysis bag with a molecular weight cut-off of 7500Da for ...

Embodiment 2

[0042] Example 2 The protective effect of Morchella fruiting body polypeptide on nerve cells

[0043] Take PC12 cells (rat pheochromocytoma, ATCC preservation number CRL1721.1) in the logarithmic growth phase at a density of 1 × 10 3 96-well plate was inoculated per well, and after 16 hours of culture, the medium was discarded and 80 μmol / L H was added. 2 o 2 Treat for 30 min, then discard the H 2 o 2 , washed 2 times with PBS, added different concentrations of Morchella polypeptide solutions and cultured for 6 hours (see Table 1 for the concentration gradient), washed with pre-cooled PBS solution and resuspended PC12 cells. Add 20 μL of 5 mg / mL 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT) to each well in the dark, CO 2 After culturing in an incubator at 37°C for 4 hours, the supernatant was sucked off, 150 μL of dimethylsulfoxide (DMSO) was added to each well, and after shaking for 10 minutes, the absorbance (OD) of each well was measured at a waveleng...

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Abstract

The invention belongs to the technical field of comprehensive utilization of edible and medical fungi, and specifically relates to a novel application of a morel fruiting body polypeptide. At present,the research on the applications of the morel fruiting body polypeptide is not enough, and in order to solve the problem, the invention provides a novel application of the morel fruiting body polypeptide in the preparation of foods or medicines for preventing or treating neurodegenerative diseases. The invention also provides applications of the morel fruiting body polypeptide in promoting neuralcell differentiation and protecting neural cells from oxidation damage caused by oxidation pressure. Specifically, the invention also provides a preparation method of the morel fruiting body polypeptide. For the first time, people find that the morel fruiting body polypeptide can well promote neural cell differentiation and protect the neural cells, is capable of effectively reducing the damage of oxidation pressure to neural cells, and can promote the neural cell differentiation and delay the aging of neural cells. A novel application of a morel fruiting body is provided.

Description

technical field [0001] The invention belongs to the technical field of comprehensive utilization of edible and medicinal fungi, and in particular relates to a new application of a hickory chick fruiting body polypeptide. Background technique [0002] Edible and medicinal fungi are a kind of biological resources with various health effects. Studies have found that the functional substances contained in edible and medicinal fungi include polysaccharides, polypeptides, nucleosides, terpenes, etc., which exhibit strong antibacterial, anti-oxidation, immune regulation, and anti-tumor effects. It has been used in certain aspects such as disease prevention and tumor prevention. In recent years, scholars at home and abroad have made in-depth research on the active substances in edible fungi, especially on the biologically active polysaccharides and active protein polypeptides in edible fungi. Edible fungi have entered people's lives as a health food. [0003] Neurodegenerative dis...

Claims

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Application Information

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IPC IPC(8): A23L33/18A23L27/10A23L23/00A23L23/10A61K38/02A61P25/28A61P25/16A61P25/14A61P25/00
CPCA61K38/02A61P25/00A61P25/14A61P25/16A61P25/28A23L23/00A23L23/10A23L27/10A23L33/18A23V2002/00A23V2200/322A23V2250/55
Inventor 熊川朱宇黄文丽金鑫陈祖琴
Owner SAAS BIOTECH & NUCLEAR TECH RES INST
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