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Apparatus for irritating animal cell and recording its physiological signal and its production and using method

A technology of animal cells and physiological signals, applied in the methods of stress-stimulating the growth of microorganisms, biochemical cleaning devices, biochemical equipment and methods, etc. Problems such as normal growth and difficulty in controlling the growth direction of neurites

Inactive Publication Date: 2007-10-24
TSINGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to control the growth direction of neurites, and they are often allowed to grow randomly, which is not conducive to the formation of effective neural networks among multiple cultured nerve cells in vitro to record the conduction of electrophysiological signals, and is also not conducive to implantation. Integration and information exchange between nerve cells and the main nervous system
Some researchers use mechanical confinement to make neurites grow in the channel, but this confinement will affect the normal growth of neurites

Method used

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  • Apparatus for irritating animal cell and recording its physiological signal and its production and using method
  • Apparatus for irritating animal cell and recording its physiological signal and its production and using method
  • Apparatus for irritating animal cell and recording its physiological signal and its production and using method

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Experimental program
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Embodiment 1

[0031] Embodiment 1, manufacture device of the present invention

[0032] On one side surface of a square glass substrate with a thickness of 350 micrometers, a layer of gold with a thickness of 300 nanometers and a measurement microelectrode with a pattern required for design is processed with conventional photolithography, and the power electrode (patterned with a polymer) partially connected) and leads.

[0033] Apply electricity to the powered electrode, and polymerize a layer of polyaniline conductive polymer with a thickness of 100 nanometers on the gold surface connected to the powered electrode by electrochemical polymerization to form the required pattern.

[0034] A layer of isolation layer is formed on the surface by spin coating, and the desired structure is formed by photolithography.

[0035] After the above steps, the device of the present invention for stimulating animal cells and recording their physiological signals can be obtained.

[0036] In this embodim...

Embodiment 2

[0037] Embodiment 2, structure of the present invention

[0038] As shown in Fig. 1, Fig. 2, Fig. 3, the device of the present invention that stimulates animal cells and records its physiological signals includes a square silicon substrate 4 with a thickness of 400 microns; Object layer 3, two electrode lead-out ends (not shown) on the conducting polymer layer 3; Be located at 16 platinum microelectrodes 2 with a thickness of 60 nanometers on the substrate 4, each microelectrode 2 is provided with an output end ( Not shown in the figure); There is a distance of 25 microns between the conductive polymer layer 3 and the microelectrode 2; the part other than the conductive polymer layer 3 on the substrate 4 and the non-cell body growth and microelectrode measurement is provided with polyimide isolation Material layer 1, the thickness of which is 10 microns, forms grooves with a width of 3-7 microns to prevent cells from escaping.

[0039] As shown in Figure 2, a small culture ca...

Embodiment 3

[0042] Embodiment 3, utilize the device of the present invention to carry out the research of neural network in vitro

[0043] As shown in Figure 4, the nerve cells 7 are placed on the device of the present invention, the conductive polymer 3 is intermittently energized, the electrical stimulation can be direct current or alternating current, and the stimulation frequency is 1-10 6 Hz; the physiological signal generated in the tested cell is measured by the microelectrode 2 .

[0044] By intermittently energizing the nerve cells (constant voltage 100mV), after culturing for several days, compare the growth of the cells in the energized group and the control group, such as cell body size, process length and other indicators. In addition, by giving stimulation to cells such as electrical and chemical signals, microelectrodes are used to measure changes in the outer membrane potential of cells to study the responses of cells to various stimuli.

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Abstract

The present invention provides an apparatus for stimulating an animal cell and recording its physiological signal and methods of making and using thereof. The purpose of the present invention is to provide an apparatus for stimulating an animal cell and recording its physiological signal that is efficient, convenient, and accurate. The apparatus for stimulating an animal cell and recording its physiological signal of the present invention comprises a poor conductive substrate, wherein on at least one surface of the substrate is provided at least one unit conductive polymer layer and at least one good conductive microelectrode.

Description

technical field [0001] The present invention relates to a device capable of stimulating animal cells and recording their physiological signals, a production method and a method for using the device, in particular to a device capable of electrically stimulating animal cells and recording their physiological signals and the device methods of production and use. Background technique [0002] Controlling the growth and differentiation of nerve cells is conducive to the in-depth study of the mechanism of the nervous system, the treatment of certain nervous system diseases, and the effective repair of nerve tissue damage. Therefore, it has been a subject of research for a long time. [0003] After years of exploration, it was found that electrical stimulation can enhance the extension of neurites in vitro and the regeneration of nerve cells in vivo. Studies have shown that the extension of neurites can be enhanced on the surface of piezoelectric materials [Aebischer, et al, Piez...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/00G01N27/30A61B5/00C12M1/42C12M1/34C12N13/00C12Q1/02G01N33/543
CPCG01N2333/46C12M35/02G01N2500/10G01N33/5438
Inventor 邢婉丽于喆项光新潘良斌程京
Owner TSINGHUA UNIV
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