43 results about "Cell brain" patented technology

The present invention relates to a composition for treating or preventing neurodevelopmental disorders, particularly autism spectrum disorder, by selectively activating brain-resident regulatory T cells (Tregs). The present composition comprises IL-2 or an IL-2 gene (AAV-GFAP-IL-2) as an active ingredient, and induces Treg proliferation in the brain to alleviate neuroinflammation and improve behavioral defects.

The application discloses application of a swertianol derivative in preparation of a glioma treatment drug and belongs to the technical field of medicines. The application finds that the compound has significant inhibitory activity on SF126, T98G, A172, U-87 MG, U251 and various glioma cell lines, and the activity is better than that of temozolomide, a commonly used clinical anti-glioma drug, and the compound has lower toxicity to normal brain cells. The application further provides a preparation method of the compound and a pharmaceutical composition containing the compound, and the pharmaceutical composition can be prepared into injection, tablets and various dosage forms. The application provides a new effective drug selection for the treatment of glioma, has a mature preparation process, various drug dosage forms and a wide clinical application prospect.

Fibrinogen-coated Albumin Nanospheres (FAS) has shown that it can mobilize bone marrow cells into the blood which then go to injured soft and hard tissues to achieve accelerated repair. It is hypothesize that (a) brain injuries caused by low-dose irradiation under simulated microgravity can be mitigated by the administration of FAS; and (b) the mechanism is from acceleration of neo-vascularization, proven by lineage study of GFP-labeled bone marrow cells implanted into compatible non-GFP animals before injury, which regenerate into functional GFP-brain cells after FAS treatment.

The present disclosure relates to LNP formulations useful for uptake in the CNS or brain, for example, in some cases wherein the LNP is conjugated to an antigen binding fragment of an anti-transferrin receptor antibody. The present disclosure also relates to methods of delivering lipid nanoparticles (LNPs) systemically, for uptake in vivo in the central nervous system (CNS), such as to brain cells, and methods for assessment of the extent of uptake in CNS cells, such as brain cells. In some cases, methods allow for high throughput screening of multiple different LNPs in parallel.

The invention discloses application of carabrone derivatives in preparation of glioma drugs, and belongs to the technical field of medicines. It is found for the first time that the compound has remarkable inhibitory activity on multiple glioma cell lines such as SF126, T98G, A172, U-87 MG and U251, the activity of the compound is superior to that of temozolomide which is a clinically common anti-glioma drug, and meanwhile the compound is low in toxicity to normal brain cells. The invention also provides a preparation method of the compound and a pharmaceutical composition containing the compound. The pharmaceutical composition can be prepared into various dosage forms such as injections, tablets and the like. The invention provides a new effective drug choice for the treatment of glioma, and has the advantages of mature preparation process, various dosage forms and wide clinical application prospect.

The invention belongs to the technical field of coffee products, and particularly relates to a coffee composition for relieving depressive symptoms and a preparation method thereof. 25-30 parts of coffee beans, 6-10 parts of fructus rosae laevigatae, 6-10 parts of semen boitae, 8-12 parts of cortex albiziae and 1-3 parts of seaweed are limited, and different raw materials are extracted, decocted or subjected to enzymolysis, so that separation and retention of active ingredients of the raw materials are facilitated, and the active ingredients in fructus rosae laevigatae, semen boitae, cortex albiziae and seaweed have a protection effect on brain cells; the traditional Chinese medicine composition is beneficial to recovering the balance of a nervous system, relieving the inflammatory response in the brain, reducing the generation of oxygen free radicals, reducing the activity of xanthine oxidase in the cerebral cortex and improving the symptom of depression; and chlorogenic acid, nicotinic acid and the like in coffee can generate positive influence on depression symptoms. Therefore, the coffee composition provided by the invention can be used for relieving depression symptoms and provides a basis for adjuvant therapy of depression.

The invention discloses a composition for enhancing memory and improving cognitive function and application. The composition disclosed by the invention comprises acanthopanax, ginseng, Chinese wolfberry fruits, gastrodia elata, spina date seeds, poria cocos, Chinese yams, lotus seeds, gordon euryale seeds, mulberries, longan pulp, fresh ginger, walnut powder, egg protein powder, blueberry powder, raspberry powder, pumpkin seed powder, oat powder, spirulina powder, dark chocolate powder, a vitamin B group compound, gamma-aminobutyric acid, galactooligosaccharide and citric acid. According to the invention, a multi-channel and multi-target collaborative network is constructed by taking acanthopanax senticosus as a core, through multiple channels such as nerve regeneration, energy metabolism, oxidative stress, signal transduction, endocrine regulation and the like, different nodes such as neurons, neuroglial cells, cerebrovascular endothelium and the like are targeted, the overall optimization of the memory function is realized on the system level, and the memory function is optimized. The core idea of systematic biology of overall regulation and control is reflected, and the composition prepared from the composition has the effects of improving memory, learning ability and cognitive ability.

The present invention relates to use of PAK4 and CRTC1 or the treatment or diagnosis of a degenerative brain disease. Specifically, the present invention relates to a pharmaceutical composition for the prevention or treatment of a degenerative brain disease comprising PAK4 (p21-activated kinase 4) or a PAK4 activator as an effective ingredient. In addition, the present invention relates to a pharmaceutical composition for the prevention or treatment of a degenerative brain disease comprising a CRTC1 expression enhancer or activator as an effective ingredient, a diagnostic kit for a degenerative brain disease comprising an agent for detecting CRTC1, and a method for screening a substance for the prevention or treatment of a degenerative brain disease, comprising (a) applying a candidate drug to brain tissue or brain cells containing CRTC1 gene or CRTC1 protein; (b) measuring the degree of phosphorylation in CRTC1; and (c) determining the candidate drug as a substance for the prevention or treatment of a degenerative brain disease when the measurement in step (b) indicates that the phosphorylation in CRTC1 is increased.

Aspects of the disclosure relate to compositions and methods for delivering a transgene (e.g., a transgene encoding one or more gene products) to a target cell (e.g., a brain cell). The disclosure is based, in part, on adeno-associated virus (AAV) capsid proteins comprising one or more amino acid substitutions, and methods of using the same for delivery of a transgene to tissues and cells of the central nervous system (e.g., brain cells).

The application provides a fusion protein, a gene, a recombinant vector, a recombinant cell, brain-targeting exosomes and application thereof, and relates to the field of drug treatment. The exosomes can be precisely targeted to a target site as a drug delivery carrier to play a therapeutic role of genes and chemical drugs. In the application, a gene sequence of a highly expressed NCAM gene in microglial cells, a segment of a simulation peptide NMP2, and a gene sequence of an exosome membrane protein Lamp2b are connected, an expression vector is constructed, and the expression vector is transfected into a packaging cell, so that the NCAM simulation peptide NMP2 is presented on the surface of the exosomes, the exosomes have the ability of targeting microglial cells, the exosomes can carry therapeutic drugs to pass through the blood-brain barrier and enter the lesion area in the brain, regulate the microenvironment, promote the clearance of A beta deposition, and can be used for the treatment of Alzheimer's disease.

This invention discloses the application of GRP94 protein as a drug delivery target in the construction of drug carriers for Parkinson's disease. GRP94 is highly upregulated on the cell membranes of dopaminergic neurons in the substantia nigra of Parkinson's disease cells, while its expression is extremely low on the surface of normal brain cells. The invention also discloses the application of GRP94 as a target in a targeted delivery system for anti-Parkinson's disease drugs. By targeting GRP94, the delivered anti-Parkinson's drug can efficiently penetrate the blood-brain barrier to reach the brain parenchyma, avoiding normal brain cells and specifically targeting dopaminergic neurons in the substantia nigra of Parkinson's disease cells, thus achieving targeted drug delivery for Parkinson's disease. This invention applies GRP94 as a target in the construction of drug carriers for Parkinson's disease and in the preparation of targeted delivery systems for anti-Parkinson's disease drugs. Compared to other target receptors that cross the blood-brain barrier for targeted brain delivery, targeting GRP94 more specifically targets dopaminergic neurons in the substantia nigra, avoiding drug accumulation and neurotoxicity in normal intracranial regions.

There is provided a system and method for generating a mapping of local brain cell activity and connectivity. The method including: receiving brain imaging data from image captures of a brain; generating a binary segmentation map using a trained deep learning model, the deep learning model taking the brain imaging data as input; performing registration of the brain imaging data to a brain template; voxelizing the segmentation map and warping the voxelized segmentation map using deformations obtained during registration; determining group-wise heatmaps of neuronal density or cellular density from the voxelized and warped segmentation map; generating a map of clusters of between-group differences in neuronal density or cellular density from the group- wise heatmaps of the voxelized and warped segmentation map; and outputting the map of clusters as a representation of local brain cell activity and connectivity.

The application provides a brain function improving brain peptide hydrolysate drink composition and a preparation method and application thereof, relates to the technical fields of peptide drinks and medicines. The brain protease hydrolysate concentrate, walnut peptides and intestinal peptides are matched and the dosages thereof are preferably selected, so that the drink composition is fully absorbed, the nervous system is better activated, the excitability of brain cells is improved, the growth and lengthening of nerve synapses are stimulated, the repair of brain nerves is helped, the gastrointestinal movement is enhanced, the gastrointestinal mucosa is better protected, the burden of the spleen and stomach is reduced, and the application is especially suitable for patients with weak spleen and stomach.

The utility model relates to the technical field of ice caps, in particular to an anti-slip ice cap which comprises a cap body and a neck supporting protection pad, the cap body comprises a double-layer rubber layer, an annular opening is formed in the inner side of the double-layer rubber layer, an invisible zipper is fixed to the edge of the annular opening, a plurality of ice columns are arranged in an interlayer of the double-layer rubber layer, and the neck supporting protection pad is arranged in the interlayer of the double-layer rubber layer. A fixing piece is further fixed in the interlayer of the double-layer rubber layer, by arranging the fixing piece, each ice column is fixed in the placing hole of the heat preservation layer, ice blocks are prevented from sliding, moving and gathering to a certain position when a patient turns over or moves, chilblain is reduced, meanwhile, the brain oxygen consumption can be reduced, brain cells can be protected, and the patient can feel comfortable. And by arranging the containing bag, the double rubber layers and the sealing ring, the conditions of ice column melting and liquid leakage can be avoided, the ice column fusion time is shortened, and the comfort level of a patient is improved.

Provided herein are, inter alia, methods and compositions for transfecting a brain cell (e.g., neuron). The methods for transfecting a brain cell provided herein including embodiments thereof include, for example, transfecting the brain cell with a viral vector encoding a potassium-selective light-sensitive protein (e.g., kalium channelrhodopsin). Also provided herein are, inter alia, methods of modulating a neural network of brain cells (e.g., neurons) by activating a potassium-selective channelrhodopsin with light. The methods and compositions provided herein are, inter alia, useful for treating neurological or psychiatric diseases or disorders (e.g., epilepsies, Parkinson's disease, Alzheimer's, etc.).

[Problem] To provide amyloid β accumulation model cells. [Solution] A neurodegenerative disease model tissue and model animal, comprising brain cells derived from a non-human animal in which all or a part of a liver is replaced with human liver cells.

The invention relates to long-acting hydrogen release particles for targeted anchoring of apoplexy inflammation blood vessels as well as a preparation method and application of the long-acting hydrogen release particles. The preparation method comprises the following steps: 1.1) completely and uniformly mixing ZrSiraw material powder, a dispersing agent and a solvent in a mass ratio of 100: (50-1000): (10-10000); 1.2) transferring the mixture into a ball mill for ball milling at a ball milling speed of 200-1000 revolutions per minute for 1-7 days; 1.3) carrying out graded centrifugation on the ball-milled solution, and collecting particles with the particle size of 50-200 nanometers; and 1.4) washing, centrifuging again to obtain the required ZrSi nano-particles, dispersing the ZrSi nano-particles into a solvent, and storing in a nitrogen environment. In cerebral apoplexy, the nanoparticles anchored on inflammatory blood vessels do not need to pass through the blood brain barrier, so that potential safety risks caused by the nanoparticles to brain parenchyma are avoided. And moreover, Hs can be generated in local hydrolysis, and then the Hs pass through the blood brain barrier, so that safe and effective brain cell protection is realized. And hydrogen can be continuously released, so that continuous treatment is realized, and accumulation of nanoparticles in brain parenchyma is avoided.

The invention provides a high-resolution microelectrode neuron stimulation system. The high-resolution microelectrode neuron stimulation system comprises at least two stimulation electrodes, a first control module and a second control module, the peripheries of the stimulating electrodes are connected to form a stimulating area; the first control module is provided with a first signal source and at least two first switches. The first signal source provides a first current with a first frequency. The second control module is provided with a second signal source and at least two second switches, the second signal source provides a second current with a second frequency, the current intensity of any two electrodes can be controlled through the structure, brain cell tissue at a target position in a stimulation area is selectively stimulated, and the stimulation efficiency is improved. According to the invention, the electrode setting complexity and the setting cost can be reduced, and the effect of high-precision neuron stimulation is achieved.

The present invention provides a device for treating Alzheimer's disease comprising elements for repairing the mitochondrial network structure of brain cells in a patient with Alzheimer's disease. The device disclosed by the invention can be used for improving cognitive impairment of a patient through intervention of a plurality of targets aiming at various pathogenic mechanisms of the Alzheimer's disease. After the composition is applied to a patient, not only can the mitochondrial network structure of brain cells in the patient be repaired, but also the concentration of A beta protein (reduced by 25%), the concentration of p-tau protein (reduced by 40%) and the concentration of caspase-3 protein (reduced by 36%) in the brain can be reduced, and gene expression related to development of the Alzheimer's disease can be partially recovered. In addition, the device performs treatment through gamma NIR light, and has the advantages of being non-invasive and high in safety.

The invention relates to the technical field of plastic brain toxicity detection, and discloses a plastic brain toxicity detection method, which comprises the following steps: giving a preset number of food particles and water to mice in a target environment, and randomly dividing the mice into groups; after the mice are weighed, MNP with a preset concentration is administered to the experimental group regularly every day, normal saline is administered to the control group, 3D behavioral recording is conducted on the mice after several days of continuous administration, and behavioral analysis is conducted according to the recording result; and after behavioral analysis, performing injection anesthesia on the mice in each group, sampling brain tissues of the mice in each group, and performing brain cell analysis based on the brain tissues of the mice so as to detect the plastic brain toxicity. The toxicity of micro-plastic or nano-plastic can be efficiently and accurately detected through systematic experiment steps.

The application provides a construction method of a zebrafish model of diabetic cerebral hemorrhage sequelae for high-throughput drug screening, and belongs to the technical field of biotechnology.The first zebrafish model capable of comprehensively simulating the complex pathological phenotypes of clinical diabetic cerebral hemorrhage sequelae is successfully constructed, and the blank of the research model in the field is filled.The pathological changes of the model are highly consistent with the symptoms of the patients with clinical diabetic cerebral hemorrhage sequelae, such as glucose metabolism disorder, pancreatic beta cell damage, cerebral vascular injury and hemorrhage, brain cell apoptosis, inflammatory reaction and behavior abnormality, and the limitation that the existing single disease model cannot simulate the synergistic pathogenesis of diabetes and cerebral hemorrhage is overcome, so that a reliable experimental animal model is provided for high-throughput screening of active ingredients against the disease.

An apparatus for measuring brain cell activity in artificial blood circulation according to an embodiment of the present invention may include a measuring unit (10) that measures EEG signals; an analog-to-digital converter (ADC) 20 that converts the EEG signals measured at the measuring unit 10 into digital signals; a control unit 50 that calculates EEG parameters from the digital EEG signals converted at the ADC 20, and calculates end-tidal carbon dioxide tension and cerebral blood flow from the EEG parameters.

The invention relates to application of an MMP-7 inhibitor to treatment of acute ischemic stroke diseases, and belongs to the technical field of biological medicines, and the MMP-7 inhibitor is 7-hydroxy-6, 8-dimethoxybenzopyran-2-one (isofraxidin). The invention discloses the application of the MMP-7 inhibitor isofraxidin in improvement and treatment of the acute ischemic stroke for the first time, and experiments prove that the cerebral infarction area, nerve cell injury and brain cell apoptosis of the acute ischemic stroke can be remarkably reduced by giving the isofraxidin. Experimental results show that Isofraxidin has an obvious improvement effect on acute ischemic stroke, and has important significance on prevention and treatment of acute ischemic stroke clinically.

Provided is a method for preparing a composition for culturing a brain organoid, the method comprising (a) decellularizing brain tissue; (b) drying the brain tissue; and (c) gelating the brain tissue.

The optimized drug delivery system, with a diameter of less than 150 nm, can cross the Blood-Brain Barrier (BBB) and effectively target brain cells. This all-natural herbal formulation contains Curcumin from Turmeric and Boswellic acids from Frankincense, encapsulated in a vesicular structure. The surface of vesicular structure has been modified and optimized for maximum bioavailability. This Nanocarrier is used for prevention of Alzheimer's disease and memory enhancement. Animal studies confirmed its effectiveness, showing positive results.

The present invention provides high-content and high-resolution in vivo screens for mapping genes that are linked to changes in cytoarchitecture or spatial transcriptomics of brain cells (e.g., neurons). The multiplex gene mapping platform utilizes genetic perturbations that are delivered to a CRISPR-expressing transgenic system via viral vectors (e.g., lentiviral vectors or AAV vectors). Phenotypic changes in the cytoarchitecture or spatial transcriptomics resulting from genetic perturbations are examined in individual brain cells, which can then be correlated with specific target genes that are modulated by the genetic perturbations.

The invention discloses a composition for repairing neural pathways and delaying senescence of cerebral nerve cells and a preparation method of the composition, and belongs to the technical field of biological medicines and functional foods. The composition is prepared from malus hupehensis (tea crabapple) leaves, phosphorylserine, N-acetylneuraminic acid, maltodextrin, mulberry leaf extract, L-alpha-choline alfoscerate, epigallocatechin gallate, bamboo leaf flavone, micrococcus, vitamin B1 and vitamin B2, and all the components are scientifically matched to achieve synergistic interaction. The preparation method comprises the steps of raw material pretreatment, mixing, crushing and sieving, sterilization and packaging, and finally the powder dosage form is prepared. The composition can regulate and control the nerve protection process in a multi-target mode, effectively repair damaged nerve pathways and delay cerebral nerve cell senescence, the raw materials are natural and safe, taking is convenient, the absorption efficiency is high, the preparation technology is simple and stable and suitable for industrial production, and a solution is provided for prevention and auxiliary improvement of neurodegenerative diseases.

This system provides a high-resolution microelectrode neuronal stimulation system that allows control of the current intensity of any two electrodes, selectively stimulating brain cell tissue B in a target area T within a stimulation area A, reducing the complexity and cost of electrode placement, and achieving highly precise neuronal stimulation. [Solution] The high-resolution microelectrode neuron stimulation system includes at least two stimulation electrodes 10, a first control module 20, and a second control module 30. The outer circumferences of the multiple stimulation electrodes 10 are connected to form a stimulation area A. The first control module 20 has a first signal source 21 and at least two first switches 23, the first signal source 21 providing a first current having a first frequency. The second control module 30 has a second signal source 31 and at least two second switches 33, the second signal source 31 providing a second current having a second frequency.

The invention belongs to the technical field of biological tissue sample dyeing and three-dimensional imaging, and particularly relates to an anti-reflection method and reagent suitable for rapid marking of complete organ cell construction. The anti-reflection dyeing reagent provided by the invention comprises a composite anti-reflection agent, a dyeing agent and a solvent, and the composite anti-reflection agent comprises a detergent, a super-hydrating agent and a cholesterol remover, so that a cell nucleus dye and a biomacromolecule fluorescence coupling marker can effectively mark a complete organ; and the staining time of the complete mouse brain cell construction can be shortened from 3-4 weeks to 5 days in a passive soaking manner. According to the anti-reflection method based on the reagent, complete organ cell construction marking can be rapidly achieved in a high-quality mode only through a passive soaking mode, and the method has the advantages of being easy and convenient to operate, high in universality, short in dyeing time, high in uniformity, high in signal-to-background ratio and the like; the method is suitable for three-dimensional reconstruction space positioning, cell counting and morphological research in physiological and pathological research, accurate anatomical atlas drawing and the like.

The invention belongs to the technical field of biological detection, and particularly relates to a DNA-ch / M-coated ZIF-8 / CFME electrode as well as a preparation method and application of the DNA-ch / M-coated ZIF-8 / CFME electrode. The DNA-cho / M-coated ZIF-8 / CFME electrode comprises a carbon fiber electrode, and ZIF-8 nanoparticles, a brain cell membrane and an aptamer DNA-cho are sequentially modified on the carbon fiber electrode. According to the invention, ZIF-8 nanoparticles with a dodecahedron structure grow in situ on the carbon fiber electrode, functional stabilization is carried out on ZIF-8 by using cell membrane phospholipid molecules, and meanwhile, a DNA aptamer for specific recognition of DA is inserted, so that the stability, pollution resistance, sensitivity and selectivity of the electrode are remarkably improved. The invention provides an important technical means for exploring the change of dopamine metabolism in the early stage of diabetes.