116 results about "Cell brain" patented technology

The invention provides a medicine for preventing and treating cerebral arterial thrombosis, and belongs to the technical field of neuromedicine. The medicine comprises salmon polypeptide as shown in SEQ ID NO.1 and a pharmaceutically acceptable carrier, and experimental results show that the salmon polypeptide has good safety, can effectively improve oxygen-glucose deprivation / reoxygenation injury of brain cells, and remarkably reduces neurological impairment and cerebral infarction of mice suffering from cerebral arterial thrombosis. The invention also discloses a pharmaceutical composition for treating cerebral arterial thrombosis. The pharmaceutical composition comprises the salmon polypeptide and edaravone. The composition shows a remarkable synergistic effect, compared with the single use of the salmon polypeptide or edaravone, the composition can more remarkably reduce neurological impairment and cerebral infarction, and the actual effect is obviously superior to the theoretical superposition effect. Therefore, the invention provides a novel and more effective treatment scheme for the treatment of cerebral arterial thrombosis.

The invention relates to the technical field of medical animal models, in particular to a zebrafish intestine toxicity modeling and detection method based on podophyllotoxin and application thereof, and the method comprises the following steps: S1, randomly selecting 3-day (3dpf) wild-type AB-line zebrafish after fertilization in a 6-pore plate, and treating 30 zebrafish in each pore (experimental group); s2, dissolving the podophyllotoxin in water, performing modeling, setting a normal control group, setting the volume of each pore to be 3mL, and treating at 28 DEG C for 2 days; and S3, performing intestinal histopathology analysis and intestinal apoptosis analysis on the zebrafish obtained by modeling in the step S2. The method comprises the following steps: Hamp; according to the method, the change of the tissue structure can be clearly observed, and the damage degree of the podophyllotoxin to the intestinal tract and brain tissue of the zebra fish can be intuitively evaluated. AO staining is combined with a fluorescence microscope, so that the apoptosis condition can be quantitatively analyzed; the damage mechanism of the podophyllotoxin to the brain cell structure and function is defined, and the blank of the podophyllotoxin in the aspect of brain toxicity research is filled.

The invention relates to the technical field of medical image processing, and discloses an adversarial generative network model for three-dimensional reconstruction of a brain cell-level vascular network. The model comprises a three-dimensional blood vessel feature extraction module, a dynamic attention generator, a multi-scale discriminator and the like. Characteristics are obtained through multi-modal medical image data (magnetic resonance angiography and confocal microscope scanning data), and high-precision vascular network three-dimensional reconstruction is realized by utilizing cooperative work of all the modules. The dynamic attention generator calculates the topological connection probability of the vascular branches, and the multi-scale discriminator globally and locally evaluates the reconstruction result. Meanwhile, model training is optimized through an adversarial training controller, and the blood vessel network is perfected through a capillary network completion module. According to the method, the advantages of multi-modal data are effectively fused, the reconstruction precision is improved, and powerful support is provided for research and diagnosis of brain vascular diseases.

The invention belongs to the technical field of bioengineering, and discloses a sunflower seed peptide for regulating metabolism of brain cells, the sunflower seed peptide has a biological sequence of Arg His Ala Lys Thr Pro Ser Asn Lys, and a preparation method of the sunflower seed peptide for regulating metabolism of brain cells comprises the following specific steps: S1, raw material pretreatment; s2, peeling and crushing; s3, protein extraction; s4, performing enzymolysis treatment; s5, performing enzyme deactivation treatment; s6, filtering and purifying; s7, concentrating and drying; and S8, quality detection and packaging. The sunflower seed protein is directionally decomposed into the active peptide fragment by precisely regulating and controlling compound protease and enzymolysis conditions, the active peptide fragment can be compatible with a brain cell specific receptor and penetrate through a blood brain barrier, and cell experiments and animal models prove that the peptide fragment can activate a mitochondrial respiratory chain complex, so that the mitochondrial membrane potential is increased by more than 30%, and the mitochondrial membrane potential is increased by more than 30%. Meanwhile, the activity of neurotransmitter synthetase can be enhanced, the brain health is protected, and tests further prove that the sunflower seed peptide also has the function of regulating the metabolism of brain cells.

The invention relates to the technical field of biology. The invention provides a grass carp brain cell line sensitive to grass carp reovirus type II, which is preserved in China Center for Type Culture Collection on October 19, 2024, and the preservation number is CCTCC NO: C2024101. The constructed grass carp brain cell line shows remarkable stability, and stable passage is successfully achieved for 60 times. And when GCRV-II is inoculated, an atypical cytopathic effect can be presented. In addition, the grass carp brain cell line has the capability of efficiently replicating GCRV-II, and even if blind passage is carried out for three generations, generated cytotoxicity can still cause morbidity and death of the grass carp. Therefore, a stable foundation is built for deeply exploring the pathogenic mechanism of the GCRV-II, developing vaccine development, screening antiviral drugs and the like, research in related fields is promoted to further progress, and a key cell model and a research basis are provided for development of related research and prevention and control strategies of the grass carp reovirus.

Disclosed are devices, systems, and methods for ultrasonically stimulating brain tissue using operating parameters associated with a. target region of a brain. The operating parameters can be determined, for example, by combined ultrasonic stimulation and photometry devices and methods. The operating parameters can include pulse repetition frequency, temporal compression, and intensity. Using selected operating parameters, ultrasound stimulation of the target region of the brain can excite or inhibit brain cell activity.

Provided is a high-speed variant ChRmine protein having faster kinetic properties compared to a parent ChRmine protein, where the high-speed variant ChRmine protein has one or more amino acid substitutions compared to the parent ChRmine protein. Also provided is a red-shifted variant ChRmine protein having a red-shifted spectrum compared to a parent ChRmine protein, where the red-shifted variant ChRmine protein has one or more amino acid substitutions compared to the parent ChRmine protein. Further provided is a nucleic acid encoding for a variant ChRmine protein disclosed herein as well as a genetically modified cell comprising such nucleic acid. Additionally, provided is an optogenetic method that includes genetically modifying a subject to express in the subject's brain cells a variant ChRmine protein disclosed herein, applying stimulating light to the subject's brain, and imaging the subject's brain.

The invention provides brain cell specific targeting delivery based on exosome, and the exosome secreted by 293T cells is used for engineering transformation, so that the capabilities of penetrating BBB and specifically targeting neurons are achieved. According to the present invention, the L1CAM is loaded on the exosome, the exosome is obtained by transfecting a 293T cell of a Lamp2b-L1CAM PCDH-HygroHA plasmid through a 293T-Lamp2b-L1CAM cell line, and the BBB cell-penetrating peptide 4F-T7 and the neuronal targeting protein L1CAM are loaded on the surface of the exosome so as to treat the neurodegenerative diseases such as the Alzheimer's disease and the Parkinson's disease.

The invention relates to an extracellular vesicle composition for aiding sleep as well as a preparation method and application thereof. The composition comprises two or more of passion flower extracellular vesicles, chamomile extracellular vesicles and liquorice extracellular vesicles. According to the extracellular vesicle composition, a combined formula is adopted, and the CL-level of cells is improved, the expression level of GABA related proteins is improved, the expression of brain-derived neurotrophic factors (BDNF) of the cells is promoted, and the synaptic growth of neuronal cells is promoted in a multi-dimensional combined mode that the passiflora edulis is compounded with multiple Chinese herbal medicine extracellular vesicles; the sleep-aiding pillow has the advantages that multi-aspect efficient sleep-aiding effects can be realized, sleep time can be shortened, and sleep quality can be improved. Meanwhile, the extracellular vesicle composition disclosed by the invention adopts nano-scale raw materials, has good permeability, and is more easily absorbed and ingested by cells and skin to play a role.

The invention provides a brain-function-improving brain-peptide-hydrolysate-containing beverage composition as well as a preparation method and application thereof, and relates to the technical field of peptide beverages and medicines. The brain protein zymolyte concentrated solution, the walnut peptide and the intestinal peptide are compatible, and the dosage is optimized, so that the beverage composition is fully absorbed, better helps to activate the nervous system, improve the excitability of brain cells, stimulate the growth and growth of neuronal synapses, help the repair of brain nerves, enhance gastrointestinal motility, better protect gastrointestinal mucosa and improve the immunity of the brain. The traditional Chinese medicine is especially suitable for patients with weakness of the spleen and the stomach.

Provided herein are methods of expressing syntaxin-binding protein 1 (STXBP1) in a neuron. Also provided herein is a modified adeno-associated virus (AAV) encoding a STXBP1 under the control of a promoter operable in a brain cell or neuronal cell.

The present invention relates to a human ectoderm derived brain cell characterized by the following features: (1) the intron between exons 10 and 11 of the MAPT gene encoding the Tau protein has been removed by genome-editing from one or both allele(s); and (2) at least one of the alleles of the MAPT gene of (1) carries at least one mutation enhancing Tau aggregation and at least one mutation enhancing nucleation of Tau aggregation; or (3) one allele of the MAPT gene as defined in claim 1(1) carries at least one mutation enhancing Tau aggregation, preferably in exon 10, and the other allele carries at least one mutation enhancing nucleation of Tau aggregation, preferably in exon 11.

Provided herein are methods of detecting an aggression level of a glioblastoma in a subject. In some embodiments, the methods include imaging the subject having the glioblastoma using a CEST MRI technique in the absence of introducing an exogenous MRI contrast agent into the subject to produce a test MR image of the subject. In some embodiments, the methods also include identifying an overexpression level of mannose in and / or on surfaces of unlabeled cancer stem cells of the glioblastoma of the subject in the test MR image relative to an identified expression level of mannose in and / or on surfaces of brain cells in a control MR image, wherein a high aggression level is indicated when the overexpression level comprises at least about a 1.8-fold difference and wherein a low aggression level is indicated when the overexpression level comprises less than about a 1.8-fold difference. Related aspects are also provided.

The invention relates to the technical field of biological medicines, and relates to a small molecule peptide composition for targeted repair of stroke brain cells based on stem cells. The invention designs small molecule peptides with targeted homing and differentiation regulation functions, the small molecule peptides are reasonably combined, and a drug delivery system is also constructed. The composition can guide the stem cells to accurately home to the damaged brain area, regulate and control the stem cells to be differentiated into functional brain cells, and can break through the blood brain barrier. Through testing, the treatment effect of the stem cells is remarkably improved, and the safety and effectiveness of treatment are enhanced.

The present invention relates to the field of biotechnology. The present invention provides a grass carp brain cell line sensitive to grass carp reovirus type II, which was deposited in the China Center for Type Culture Collection on October 19, 2024, with a deposit number of CCTCC NO: C2024101. The grass carp brain cell line constructed by the present invention shows remarkable stability and successfully achieved 60 stable passages. When inoculated with GCRV‑II, an atypical cytopathic effect can be exhibited. In addition, the grass carp brain cell line has the ability to efficiently replicate GCRV‑II, and even after three generations of blind transmission, the generated cytotoxin can still cause grass carp to become ill and die. Therefore, the present invention has laid a solid foundation for in-depth exploration of the pathogenic mechanism of GCRV‑II, vaccine development, and antiviral drug screening, which will help promote further progress in related research and provide a key cell model and research basis for the formulation of grass carp reovirus-related research and prevention and control strategies.

The invention provides sulfated monosialic acid ganglioside as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The structural formula of the ganglioside is 3GalNAc-4 (NeuAcS2 alpha2-3) Galbeta1-Cer, and the structural formula of the ganglioside is as shown in the specification. The sulfated monosialic acid ganglioside SJ-1 prepared by the invention has good activity, can be used for preparing medicines for promoting nerve regeneration or nourishing brain cells, and has a good application prospect.

The present invention relates to a composition for treating or preventing neurodevelopmental disorders, particularly autism spectrum disorder, by selectively activating brain-resident regulatory T cells (Tregs). The present composition comprises IL-2 or an IL-2 gene (AAV-GFAP-IL-2) as an active ingredient, and induces Treg proliferation in the brain to alleviate neuroinflammation and improve behavioral defects.

Provided herein are methods of detecting an aggression level of a glioblastoma in a subject. In some embodiments, the methods include imaging the subject having the glioblastoma using a CEST MRI technique in the absence of introducing an exogenous MRI contrast agent into the subject to produce a test MR image of the subject. In some embodiments, the methods also include identifying an overexpression level of mannose in and / or on surfaces of unlabeled cancer stem cells of the glioblastoma of the subject in the test MR image relative to an identified expression level of mannose in and / or on surfaces of brain cells in a control MR image, wherein a high aggression level is indicated when the overexpression level comprises at least about a 1.8-fold difference and wherein a low aggression level is indicated when the overexpression level comprises less than about a 1.8-fold difference. Related aspects are also provided.

The present invention provides a method of treating Alzheimer's disease by activation of AMPK with a GLP-1 receptor agonist, the method involving the use of the GLP-1 receptor agonist to activate the AMPK signaling pathway in brain cells, thereby reducing beta-secretase (BACE1) mediated APP hydrolysis and A beta protein production, and promoting phagocytosis of microglial cells, thereby reducing the AMPK signaling pathway in brain cells. Therefore, neuroinflammation is reduced and cognitive impairment is improved. The invention further provides a pharmaceutical composition containing the GLP-1 receptor agonist and a corresponding medicine preparation application.

The invention provides a construction method and device for a brain structure connection atlas, and the method comprises the steps: obtaining a three-dimensional fluorescence microscopic image of brain cells; calculating a response function according to the gradient distribution of the unidirectional fiber groups; according to the gradient weighted sampling data and the response function of each voxel v in the three-dimensional fluorescence microscopic image, constructing a brain structure connection map; the gradient weighted sampling data at each voxel v represents discrete sampling in each direction of the gradient spherical continuous distribution function within a predetermined range near each voxel v. According to the method, the three-dimensional orientation distribution of the white matter nerve fibers of the brain can be obtained through analysis, then the three-dimensional brain structure connection diagram is constructed according to the fiber orientation distribution, and the method has the advantages of being high in speed, high in precision and capable of achieving three-dimensional analysis.

The application discloses application of a swertianol derivative in preparation of a glioma treatment drug and belongs to the technical field of medicines. The application finds that the compound has significant inhibitory activity on SF126, T98G, A172, U-87 MG, U251 and various glioma cell lines, and the activity is better than that of temozolomide, a commonly used clinical anti-glioma drug, and the compound has lower toxicity to normal brain cells. The application further provides a preparation method of the compound and a pharmaceutical composition containing the compound, and the pharmaceutical composition can be prepared into injection, tablets and various dosage forms. The application provides a new effective drug selection for the treatment of glioma, has a mature preparation process, various drug dosage forms and a wide clinical application prospect.

Fibrinogen-coated Albumin Nanospheres (FAS) has shown that it can mobilize bone marrow cells into the blood which then go to injured soft and hard tissues to achieve accelerated repair. It is hypothesize that (a) brain injuries caused by low-dose irradiation under simulated microgravity can be mitigated by the administration of FAS; and (b) the mechanism is from acceleration of neo-vascularization, proven by lineage study of GFP-labeled bone marrow cells implanted into compatible non-GFP animals before injury, which regenerate into functional GFP-brain cells after FAS treatment.

The present disclosure relates to LNP formulations useful for uptake in the CNS or brain, for example, in some cases wherein the LNP is conjugated to an antigen binding fragment of an anti-transferrin receptor antibody. The present disclosure also relates to methods of delivering lipid nanoparticles (LNPs) systemically, for uptake in vivo in the central nervous system (CNS), such as to brain cells, and methods for assessment of the extent of uptake in CNS cells, such as brain cells. In some cases, methods allow for high throughput screening of multiple different LNPs in parallel.

To provide an artificial device that resembles the network of nerve fibers in biology.SOLUTION: Nerve fibers extending from the human brain cells have fractured surfaces called synapses and form a small electric current called an impulse in the gaps generated at the fractured surfaces. As this formation is repeated at the synapses, the small electric current of the impulse is combined and increases in power. As a result, the brain cells continue to secure an infinitely stable power source. This fact certainly exists. In other words, the brain's power source development continues until the human dies, until the heart stops, and, paradoxically, if a human does not die, the power source will continue to be secured forever. How is the electric current that constitutes this power source created in the brain. Focusing on the structure of the nerve fibers of the brain, an artificial device similar to the network of nerve fibers in biology has been realized by applying engineering and physics to the study.SELECTED DRAWING: Figure 1

D-serine is effective for inhibiting neuron loss due to an immune response by glial cells to a brain injury. An example of a method includes artificially administering a composition including D-serine to brain cells after an injury thereto, the composition including an amount of D-serine effective to inhibit death of neurons.

The invention discloses a tracheal catheter device with rotary permanent magnet positioning and selective brain cooling functions and a temperature control method of the tracheal catheter device. A plurality of flexible semiconductor chilling plates and a plurality of temperature and humidity sensors are embedded in the inner wall side and the portion, close to the outer wall side, of a flexible tracheal catheter in the catheter extending direction; the function of heating wet gas entering the tracheal catheter is achieved; the outer wall of the trachea cannula is cooled, a local low-temperature environment is achieved, and therefore a certain protection effect on the brain is achieved. The low temperature reduces the metabolic rate of brain cells, reduces oxygen consumption of the brain, contributes to relieving brain injury and improves the brain function recovery effect of a patient; the flexible semiconductor chilling plate and the temperature and humidity sensor are used for accurate temperature control, accurate adjustment of the temperature of the outer wall of the trachea cannula is achieved, adverse effects on the brain protection effect caused by too large temperature fluctuation are avoided, and the method is more targeted and controllable compared with a traditional cooling method.

The invention provides a method for preparing a composition capsule containing a sea squirt extract, DHA and NMN, and relates to the technical field of health-care food. The composition capsule is composed of the sea squirt extract, free docosahexaenoic acid, nicotinamide mononucleotide and vitamin E. The composition comprises the following components in parts by weight: 14-20 parts of the sea squirt extract, 20-40 parts of the free docosahexaenoic acid, 40-53 parts of the nicotinamide mononucleotide and 0.2-2 parts of the vitamin E. The plasmalogen in the sea squirt extract can improve the function of a cell membrane in brain cells and improve the immunity of the brain cells. DHA has the effect of promoting formation and development of brain cells, NMN is utilized for promoting synthesis and release of neurotransmitters to improve the function stability of a nervous system, vitamin E serves as an antioxidant and an anti-inflammatory agent to enhance nerve protection, and the composition capsule has the synergistic effect, so that the brain function is remarkably enhanced, brain aging is delayed, and the effect of improving the brain function is achieved. The senile dementia is effectively prevented and improved.

The invention discloses application of carabrone derivatives in preparation of glioma drugs, and belongs to the technical field of medicines. It is found for the first time that the compound has remarkable inhibitory activity on multiple glioma cell lines such as SF126, T98G, A172, U-87 MG and U251, the activity of the compound is superior to that of temozolomide which is a clinically common anti-glioma drug, and meanwhile the compound is low in toxicity to normal brain cells. The invention also provides a preparation method of the compound and a pharmaceutical composition containing the compound. The pharmaceutical composition can be prepared into various dosage forms such as injections, tablets and the like. The invention provides a new effective drug choice for the treatment of glioma, and has the advantages of mature preparation process, various dosage forms and wide clinical application prospect.

The invention belongs to the technical field of coffee products, and particularly relates to a coffee composition for relieving depressive symptoms and a preparation method thereof. 25-30 parts of coffee beans, 6-10 parts of fructus rosae laevigatae, 6-10 parts of semen boitae, 8-12 parts of cortex albiziae and 1-3 parts of seaweed are limited, and different raw materials are extracted, decocted or subjected to enzymolysis, so that separation and retention of active ingredients of the raw materials are facilitated, and the active ingredients in fructus rosae laevigatae, semen boitae, cortex albiziae and seaweed have a protection effect on brain cells; the traditional Chinese medicine composition is beneficial to recovering the balance of a nervous system, relieving the inflammatory response in the brain, reducing the generation of oxygen free radicals, reducing the activity of xanthine oxidase in the cerebral cortex and improving the symptom of depression; and chlorogenic acid, nicotinic acid and the like in coffee can generate positive influence on depression symptoms. Therefore, the coffee composition provided by the invention can be used for relieving depression symptoms and provides a basis for adjuvant therapy of depression.

The invention discloses a composition for enhancing memory and improving cognitive function and application. The composition disclosed by the invention comprises acanthopanax, ginseng, Chinese wolfberry fruits, gastrodia elata, spina date seeds, poria cocos, Chinese yams, lotus seeds, gordon euryale seeds, mulberries, longan pulp, fresh ginger, walnut powder, egg protein powder, blueberry powder, raspberry powder, pumpkin seed powder, oat powder, spirulina powder, dark chocolate powder, a vitamin B group compound, gamma-aminobutyric acid, galactooligosaccharide and citric acid. According to the invention, a multi-channel and multi-target collaborative network is constructed by taking acanthopanax senticosus as a core, through multiple channels such as nerve regeneration, energy metabolism, oxidative stress, signal transduction, endocrine regulation and the like, different nodes such as neurons, neuroglial cells, cerebrovascular endothelium and the like are targeted, the overall optimization of the memory function is realized on the system level, and the memory function is optimized. The core idea of systematic biology of overall regulation and control is reflected, and the composition prepared from the composition has the effects of improving memory, learning ability and cognitive ability.